A part of MilliporeSigma

01-305 | PDGF-BB Protein, human

10 µg  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
Request Pricing
Limited AvailabilityLimited Availability
Limited Quantities Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service


      Contact Customer Service

      Click To Print This Page


      Replacement Information

      Key Specifications Table

      Key ApplicationsSpeciesSource
      CULT Human Recombinant protein expressed in E.coli from the c-sis oncogene.
      Catalogue Number01-305
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionPDGF-BB Protein, human
      Product Information
      PresentationLypholized from 0.1M acetic acid
      ApplicationThe human PDGF-BB Protein is available in a 10 µg format.
      Key Applications
      • Cell Culture
      Biological Information
      SourceRecombinant protein expressed in E.coli from the c-sis oncogene.
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 7, at sites where this gene and that for COL1A1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two splice variants have been identified for this gene.
      Gene Symbol
      • PDGFB
      • FLJ12858
      • PDGF2
      • becaplermin
      • SIS
      • c-sis
      • PDGF-2
      • SSV
      • Becaplermin
      Purification MethodSterilized through a 0.2 micron membrane filter
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P01127 # Platelet-derived growth factor is a potent mitogen for cells of mesenchymal origin. Binding of this growth factor to its affinity receptor elicits a variety of cellular responses. It is released by platelets upon wounding and plays an important role in stimulating adjacent cells to grow and thereby heals the wound.
      SIZE: 241 amino acids; 27283 Da
      SUBUNIT: Antiparallel disulfide-linked dimer of non-identical (A and B) chains. Homodimers of A and B chains are implicated in transformation processes. Interacts with XLKD1 (By similarity).
      TISSUE SPECIFICITY: Expressed at high levels in the heart, brain (sustantia nigra), placenta and fetal kidney. Expressed at moderate levels in the brain (hippocampus), skeletal muscle, kidney and lung.
      DISEASE: SwissProt: P01127 # A chromosomal aberration involving PDGFB is a cause of dermatofibrosarcoma protuberans (DFSP) [MIM:607907]. Translocation t(17;22)(q22;q13) with COLA1. DFSP is an uncommon, locally aggressive, but rarely metastasizing tumor of the deep dermis and subcutaneous tissue. It typically occurs during early or middle adult life and is most frequently located on the trunk and proximal extremities.
      SIMILARITY: SwissProt: P01127 ## Belongs to the PDGF/VEGF growth factor family.
      MISCELLANEOUS: A-A and B-B, as well as A-B, dimers can bind to the PDGF receptor.
      Stem Cell Type
      • Mesenchymal Stem Cells
      • Hematopoietic Stem Cells
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by biological activity assay.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size10 µg
      Transport Information
      Supplemental Information


      Certificates of Analysis

      TitleLot Number
      PDGF-BB (Platelet Derived Growth Factor BB) (human recombinant homodimer) - 1988718 1988718
      PDGF-BB (Platelet Derived Growth Factor BB) - 2038617 2038617
      PDGF-BB (Platelet Derived Growth Factor BB) - 2049195 2049195
      PDGF-BB (Platelet Derived Growth Factor BB) - DAM1564618 DAM1564618
      PDGF-BB (Platelet Derived Growth Factor BB) - DAM1810787 DAM1810787
      PDGF-BB (Platelet Derived Growth Factor BB) - JBC1925517 JBC1925517
      PDGF-BB, human - 15661 15661
      PDGF-BB, human - 17315 17315
      PDGF-BB, human - 18403 18403
      PDGF-BB, human - 19872 19872
      PDGF-BB, human - 23566 23566
      Recombinant Human PDGF-BB - 2092640 2092640
      Recombinant Human -2558377 2558377
      Recombinant Human -2622605 2622605
      Recombinant Human -2680750 2680750
      Recombinant Human -2728394 2728394
      Recombinant Human -2757176 2757176
      Recombinant Human PDGF-BB 2470367
      Recombinant Human PDGF-BB - 2194037 2194037
      Recombinant Human PDGF-BB - 2266463 2266463
      Recombinant Human PDGF-BB - 2309022 2309022


      Reference overviewApplicationPub Med ID
      Diabetes accelerates smooth muscle accumulation in lesions of atherosclerosis: lack of direct growth-promoting effects of high glucose levels
      Suzuki, L. A., et al
      Diabetes, 50:851-60 (2001) 2001

      Cell Culture11289052 11289052
      Distinct mechanisms of activation of Stat1 and Stat3 by platelet-derived growth factor receptor in a cell-free system.
      Vignais, M L and Gilman, M
      Mol. Cell. Biol., 19: 3727-35 (1999) 1999

      Show Abstract
      Immunoprecipitation10207096 10207096
      Persistent activation of mitogen-activated protein kinases p42 and p44 and ets-2 phosphorylation in response to colony-stimulating factor 1/c-fms signaling.
      Fowles, L F, et al.
      Mol. Cell. Biol., 18: 5148-56 (1998) 1998

      Show Abstract
      Cell Culture9710599 9710599
      Platelet-derived growth factor-stimulated secretion of basement membrane proteins by skeletal muscle occurs by tyrosine kinase-dependent and -independent pathways.
      Albrecht, D E and Tidball, J G
      J. Biol. Chem., 272: 2236-44 (1997) 1997

      Show Abstract
      Immunoblotting (Western)8999929 8999929
      Galpha16 mimics vasoconstrictor action to induce smooth muscle alpha-actin in vascular smooth muscle cells through a Jun-NH2-terminal kinase-dependent pathway.
      Higashita, R, et al.
      J. Biol. Chem., 272: 25845-50 (1997) 1997

      Show Abstract
      IP-Kinase Assay9325315 9325315
      The Src SH3 domain is required for DNA synthesis induced by platelet-derived growth factor and epidermal growth factor
      Erpel, T., et al
      J Biol Chem, 271:16807-12 (1996) 1996

      Cell Culture8663328 8663328
      Differential translocation of rho family GTPases by lysophosphatidic acid, endothelin-1, and platelet-derived growth factor
      Fleming, I. N., et al
      J Biol Chem, 271:33067-73 (1996) 1996

      Cell Stimulation8955154 8955154
      Mechanism of action of platelet-derived growth factor.
      Claesson-Welsh, L
      Int. J. Biochem. Cell Biol., 28: 373-85 (1996) 1996

      Show Abstract
      9026349 9026349
      Signal transduction by the platelet-derived growth factor receptor.
      Williams, L T
      Science, 243: 1564-70 (1989) 1989

      Show Abstract
      2538922 2538922