A part of MilliporeSigma

GF310 | PDGF-BB Protein, Human Recombinant Animal Free

GF310
10 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      SourcePurity
      E. coli Product is endotoxin free (< 0.1ng/μg of protein (<1EU/μg) and greater than 98% pure by SDS-PAGE gel and HPLC analyses
      Description
      Catalogue NumberGF310
      DescriptionPDGF-BB Protein, Human Recombinant Animal Free
      Alternate Names
      • Platelet Derived Growth Factor-BB
      • Platelet Derived Growth Factor
      • PDGF-2
      • Platelet-derived growth factor B chain
      • Platelet-derived growth factor beta polypeptide
      • Proto-oncogene c-Sis
      Background InformationPlatelet derived growth factor (PDGF) was discovered as a major mitogenic factor present in serum but absent from plasma. It was found to be secreted from the αgranules of platelets activated during the coagulation of blood to form serum. Subsequent studies have demonstrated that PDGF is not one molecule but three, each a dimeric combination of two distinct but structurally related peptide chains designated A and B. The dimeric isoforms PDGFAA, AB and BB are differentially expressed in various cell types and their effects are mediated through two distinct receptors, termed α and β. The PDGFs are stored in platelet α-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-α and PDGFR-β. PDGFR-α is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-β interacts with only PDGF-BB and PDGF-AB. Recombinant human PDGF-BB is a 24.3 kDa disulfide-linked homodimer of two B chains (218 total amino acids).
      References
      Product Information
      Formulation
      PresentationProduct is filtered through a 0.2 micron filter before lyophilization.
      Applications
      ApplicationThe Platelet derived growth factor (PDGF) was discovered as a major mitogenic factor present in serum but absent from plasma. Recombinant animal free human PDGF-BB is manufactured using all non-animal reagents.
      Biological Information
      PurityProduct is endotoxin free (< 0.1ng/μg of protein (<1EU/μg) and greater than 98% pure by SDS-PAGE gel and HPLC analyses
      SourceE. coli
      Cross ReactivityCow, Monkey, Mouse, Pig, Rat
      UniProt Number
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceBioactivity assay: Determined by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells. The expected ED50 for this effect is 1.0-3.0 ng/ml.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -20°C for up to 6 months from date of receipt.
      Packaging Information
      Material Size10 µg
      Transport Information
      Supplemental Information
      Specifications

      Related Products & Applications

      Product Families

      Categories

      Life Science Research > Proteins and Enzymes > Cytokines & Growth Factors
      Life Science Research > Cell Culture and Systems > Cell Culture Media and Supplements > Cell Culture Supplements > Cytokines & Growth Factors