521225 PDGF, BB Homodimer, Human, Recombinant, E. coli

521225
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      Overview

      Replacement Information

      Key Specifications Table

      Purity
      ≥95% by SDS-PAGE

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      521225-10UG
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          Plastic ampoule 10 μg
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          Description
          OverviewRecombinant, human platelet-derived growth factor (disulfide-linked dimer of two 109 amino acid B-chain monomers) expressed in E. coli. Shown to be a potent chemoattractant for neutrophils, mesenchymal and mononuclear cells. Has the same amino acid sequence as mature human PDGF-B. Rapidly activates protein kinase D in vascular smooth muscle cells.
          Catalogue Number521225
          Brand Family Calbiochem®
          SynonymsrhPDGF-BB
          References
          ReferencesAbedi, H., et al. 1998. FEBS Lett. 427, 209.
          Abboud, H.E., et al. 1994. J. Cell Physiol. 158, 140.
          Westermark, B., and Heldin, C.-H. 1991. Cancer Res. 51, 5087.
          Raines, E.W., et al. 1985. Methods Enzymol. 109, 749.
          Johnson, A., et al. 1984. EMBO J. 3, 921.
          Product Information
          FormLyophilized
          FormulationLyophilized from a sterile-filtered solution, carrier free.
          Applications
          Biological Information
          Biological activityED₅₀ = 1-5 ng/ml as determined by the dose dependent proliferation of BALB/c 3T3 cells
          Purity≥95% by SDS-PAGE
          Physicochemical Information
          ContaminantsEndotoxin: ≤1 ng/µg PDGF-BB
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage ≤ -70°C
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 3 months at -70°C.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          521225

          References

          Reference overview
          Abedi, H., et al. 1998. FEBS Lett. 427, 209.
          Abboud, H.E., et al. 1994. J. Cell Physiol. 158, 140.
          Westermark, B., and Heldin, C.-H. 1991. Cancer Res. 51, 5087.
          Raines, E.W., et al. 1985. Methods Enzymol. 109, 749.
          Johnson, A., et al. 1984. EMBO J. 3, 921.

          Brochure

          Title
          Growth Factors and Cytokines Technical Bulletin
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision01-October-2007 JSW
          SynonymsrhPDGF-BB
          DescriptionRecombinant, human platelet-derived growth factor (disulfide-linked dimer of two 109 amino acid B-chain monomers) expressed in E. coli. Shown to be a potent chemoattractant for neutrophils, mesenchymal, and mononuclear cells. PDGF represents one of the major mitogens present in platelets. It exerts its effects by binding to specific high-affinity dimeric receptor tyrosine kinases. Material has the same amino acid sequence as mature human PDGF-B. Two distinct PDGF receptors have been identified: the PDGFα receptor, which binds to all three forms of PDGF, and the PDGFβ receptor, which can bind only PDGF-BB and PDGF-AB.
          FormLyophilized
          FormulationLyophilized from a sterile-filtered solution, carrier free.
          Purity≥95% by SDS-PAGE
          ContaminantsEndotoxin: ≤1 ng/µg PDGF-BB
          Biological activityED₅₀ = 1-5 ng/ml as determined by the dose dependent proliferation of BALB/c 3T3 cells
          SolubilityReconstitute to a concentration of ≥100 µg/ml 100 mM acetic acid, 0.1% BSA. Further dilute with low endotoxin medium or buffered solution containing FBS or tissue culture grade BSA just prior to use.
          Storage ≤ -70°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 3 months at -70°C.
          Toxicity Standard Handling
          Merck USA index14, 7525
          ReferencesAbedi, H., et al. 1998. FEBS Lett. 427, 209.
          Abboud, H.E., et al. 1994. J. Cell Physiol. 158, 140.
          Westermark, B., and Heldin, C.-H. 1991. Cancer Res. 51, 5087.
          Raines, E.W., et al. 1985. Methods Enzymol. 109, 749.
          Johnson, A., et al. 1984. EMBO J. 3, 921.

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          Categories

          Life Science Research > Proteins and Enzymes > Cytokines & Growth Factors
          Life Science Research > Cell Culture and Systems > Cell Culture Media and Supplements > Cell Culture Supplements > Cytokines & Growth Factors