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14-309 | PCAF Protein, Active, 50 µg

50 µg  
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      Replacement Information

      Key Specifications Table

      Key ApplicationsEntrez Gene NumberUni Prot Number
      HAT NM_003884.3 Q92831
      Catalogue Number14-309
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionPCAF Protein, Active, 50 µg
      OverviewRecombinant GST fusion protein
      Product Information
      ApplicationPCAF recombinant GST fusion protein, for use in Histone Acetyltransferase Assays. (HAT).
      Key Applications
      • Histone Acetyltransferase Assay (HAT)
      Biological Information
      Sourceexpressed in E. Coli corresponding to amino acids 352-832
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Entrez Gene Number
      Entrez Gene SummaryCBP and p300 are large nuclear proteins that bind to many sequence-specific factors involved in cell growth and/or differentiation, including c-jun and the adenoviral oncoprotein E1A. The protein encoded by this gene associates with p300/CBP. It has in vitro and in vivo binding activity with CBP and p300, and competes with E1A for binding sites in p300/CBP. It has histone acetyl transferase activity with core histones and nucleosome core particles, indicating that this protein plays a direct role in transcriptional regulation.
      Gene Symbol
      • PCAF
      • GCN5
      • GCN5L
      • P
      • P/CAF
      • CAF
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q92831 # Functions as a histone acetyltransferase (HAT) to promote transcriptional activation. Has significant histone acetyltransferase activity with core histones (H3 and H4), and also with nucleosome core particles. Inhibits cell-cycle progression and counteracts the mitogenic activity of the adenoviral oncoprotein E1A. In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes.
      SIZE: 832 amino acids; 93013 Da
      SUBUNIT: Interacts with SIRT1 (By similarity). Interacts with EP300 and CREBBP. Interacts with NCOA1 and NCOA3. Binds to HTLV-1 Tax. Interacts with and acetylates HIV-1 Tat.
      SUBCELLULAR LOCATION: Nucleus (By similarity).
      TISSUE SPECIFICITY: Ubiquitously expressed but most abundant in heart and skeletal muscle.
      DOMAIN:SwissProt: Q92831 The bromodomain mediates binding to HIV-1 Tat.
      SIMILARITY: Belongs to the GCN5 family. & Contains 1 bromo domain. & Contains 1 N-acetyltransferase domain.
      Molecular Weight79kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by acetylation of core histones in vitro
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions6 months at -20°C
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      PCAF, active (recombinant protein expressed in E. coli) - 2297436 2297436
      PCAF, active (recombinant protein expressed in E. coli) - 2398188 2398188
      PCAF, active (recombinant protein expressed in E. coli) - JBC1855998 JBC1855998
      PCAF, active - 18140 18140
      PCAF, active - 21403 21403
      PCAF, active - 23030 23030
      PCAF, active - 23979 23979
      PCAF, active - 25150 25150
      PCAF, active - 28130 28130
      PCAF, active - 31782 31782
      PCAF, active - DAM1601743 DAM1601743
      PCAF, active - DAM1718554 DAM1718554
      PCAF, active - DAM1776537 DAM1776537
      PCAF, active - R0609D0005 R0609D0005
      PCAF, active -2484115 2484115
      PCAF, active -2553663 2553663
      PCAF, active -2704898 2704898


      Reference overviewPub Med ID
      The chromatin-binding protein HMGN3 stimulates histone acetylation and transcription across the Glyt1 gene.
      Barkess, G; Postnikov, Y; Campos, CD; Mishra, S; Mohan, G; Verma, S; Bustin, M; West, KL
      The Biochemical journal 442 495-505 2012

      Show Abstract
      22150271 22150271
      Acetylation-dependent interaction of SATB1 and CtBP1 mediates transcriptional repression by SATB1.
      Purbey, Prabhat Kumar, et al.
      Mol. Cell. Biol., 29: 1321-37 (2009) 2009

      19103759 19103759
      Construction and expression of a single chain Fv fragment against pharmacologically active paeoniflorin in Escherichia coli, and its potential use in an enzyme-linked immunosorbent assay.
      Zhaohua Lu,Tadashi Masaki,Yukihiro Shoyama,Hiroyuki Tanaka
      Planta medica 72 2006

      Show Abstract
      16491451 16491451
      Chromosomal protein HMGN1 enhances the acetylation of lysine 14 in histone H3.
      Jae-Hwan Lim,Katherine L West,Yaffa Rubinstein,Michael Bergel,Yuri V Postnikov,Michael Bustin
      The EMBO journal 24 2005

      Show Abstract Full Text Article
      16096646 16096646
      p53 sites acetylated in vitro by PCAF and p300 are acetylated in vivo in response to DNA damage.
      Liu, L, et al.
      Mol. Cell. Biol., 19: 1202-9 (1999) 1999

      Show Abstract
      9891054 9891054
      Overlapping but distinct patterns of histone acetylation by the human coactivators p300 and PCAF within nucleosomal substrates
      Schiltz, R L, et al
      J Biol Chem, 274:1189-92 (1999) 1999

      9880483 9880483