71491 Overnight Express™ Instant TB Medium - Novagen

71491
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service
      View Pricing & Availability

      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      71491-3
      Retrieving availability...
      Limited AvailabilityLimited Availability
      Stocked 
      Discontinued
      Limited Quantities Available
      Available
        Remaining : Will advise
          Remaining : Will advise
          Will advise
          Contact Customer Service
          Contact Customer Service

          Plastic ampoule 1 easy
          Retrieving price...
          Price could not be retrieved
          Minimum Quantity needs to be mulitiple of
          Upon Order Completion More Information
          You Saved ()
           
          Request Pricing
          71491-4
          Retrieving availability...
          Limited AvailabilityLimited Availability
          Stocked 
          Discontinued
          Limited Quantities Available
          Available
            Remaining : Will advise
              Remaining : Will advise
              Will advise
              Contact Customer Service
              Contact Customer Service

              Glass bottle 5 easy
              Retrieving price...
              Price could not be retrieved
              Minimum Quantity needs to be mulitiple of
              Upon Order Completion More Information
              You Saved ()
               
              Request Pricing
              71491-5
              Retrieving availability...
              Limited AvailabilityLimited Availability
              Stocked 
              Discontinued
              Limited Quantities Available
              Available
                Remaining : Will advise
                  Remaining : Will advise
                  Will advise
                  Contact Customer Service
                  Contact Customer Service

                  Plastic ampoule 1 kg
                  Retrieving price...
                  Price could not be retrieved
                  Minimum Quantity needs to be mulitiple of
                  Upon Order Completion More Information
                  You Saved ()
                   
                  Request Pricing
                  71491-60GM
                  Retrieving availability...
                  Limited AvailabilityLimited Availability
                  Stocked 
                  Discontinued
                  Limited Quantities Available
                  Available
                    Remaining : Will advise
                      Remaining : Will advise
                      Will advise
                      Contact Customer Service
                      Contact Customer Service

                      Glass bottle 60 gm
                      Retrieving price...
                      Price could not be retrieved
                      Minimum Quantity needs to be mulitiple of
                      Upon Order Completion More Information
                      You Saved ()
                       
                      Request Pricing
                      71491-EA
                      Retrieving availability...
                      Limited AvailabilityLimited Availability
                      Stocked 
                      Discontinued
                      Limited Quantities Available
                      Available
                        Remaining : Will advise
                          Remaining : Will advise
                          Will advise
                          Contact Customer Service
                          Contact Customer Service

                          Glass bottle
                          Retrieving price...
                          Price could not be retrieved
                          Minimum Quantity needs to be mulitiple of
                          Upon Order Completion More Information
                          You Saved ()
                           
                          Request Pricing
                          Description
                          Overview

                          Overnight Express™ Instant TB Medium is a granulated autoinduction culture medium for high-level protein production in pET and other IPTG-inducible bacterial expression systems. Instant TB Medium is a combination of Overnight Express Autoinduction System 1 components (Cat. No. 71300) with rich Terrific Broth (TB) medium and is supplied in two formats.
                          EasyPak is an aluminum foil pouch containing 60 g granulated medium, sufficient for 1 L culture. Just add the EasyPak contents to 1 L sterile water, supplement with 10 ml glycerol, and microwave for 2 min to dissolve. Overnight Express Instant TB Medium is also available in 1 kg bottles. Granulated medium ensures rapid, uniform dissolution in water, prevents clumping of the medium, and avoids inhalation of airborne powder.

                          Overnight Express™ Autoinduction media are based on technology developed by F. William Studier at Brookhaven National Laboratory and enable regulated protein expression in E. coli,without monitoring the culture or adding IPTG during cell growth. The method uses media components that are metabolized differentially to promote growth to high cell density and automatically induce protein expression from lac promoters. As a result, these unique autoinduction media provide high-level protein production in pET and other IPTG-inducible bacterial expression systems.

                          Cell mass and target protein yield are often increased several-fold compared to conventional protocols using induction with IPTG. Autoinduction media are extremely convenient for routine expression of proteins in multiple cultures and are ideal for high-throughput parallel analysis of protein expression, solubility, and purification from multiple expression clones




                          EasyPak is an aluminum foil pouch containing 60 g granulated medium sufficient for 1 L culture. Just add the EasyPak contents to 1 L sterile water, supplement with 10 ml glycerol, and microwave for 2 min. Overnight Express Instant TB Medium is also available in 1 kg bottles.


                          In addition to Instant TB Medium, a variety of Overnight Express Autoinduction Media are available for different methods:

                          Overnight Express™ Autoinduction System 1 (71300) contains 3 sterile solutions, which can be added to your choice of glucose-free bacterial growth medium.

                          Overnight Express™ Autoinduction System 2 (71366) prepares a defined bacterial growth medium that can be used to selenomethionine-label proteins for x-ray crystallography.


                          Overnight Express Autoinduction NMR Media are 3 different media designed for cell growth Optimization (71760), 15N (71759) protein labeling, and 15N, 13C (71789) dual protein labeling for NMR analysis.
                          PRODUCT USE RESTRICTIONS - PRODUCT LABEL LICENSE

                          The Overnight Express™ Auto Induction Media material (Products) includes patented technology embodied therein developed at Brookhaven National Laboratory under contract with the U.S. Department of Energy. The patent applications or issued patents embodied in this Product were assigned to Brookhaven Science Associates, LLC (BSA). BSA has enabled EMD Millipore and its Affiliates to grant a non-exclusive license for use of this patented technology, including use with the enclosed Products, based upon your acceptance of the following terms:
                          1. These Products are to be used for non-commercial research purposes only. For the avoidance of doubt, such non-commercial research purposes include research use by academic, non-profit, and commercial entities.
                          2. A separate license is required for any commercial use, including the use of the enclosed Products for developing or making other materials for sale to third parties.
                          3. Information about licenses for commercial use of the Products may be obtained from:

                          Office of Technology Commercialization and Partnerships
                          Brookhaven National Laboratory
                          Building No. 490C - P.O. Box 5000
                          Upton, New York 11973-5000
                          Telephone: 631-344-4711
                          Fax: 631-344-3729
                          E-mail: pupadhya@bnl.gov


                          4. Information about licenses for commercial manufacture and/or sale of the Products may be obtained from:

                          Intellectual Property Department
                          EMD Millipore Corporation
                          28820 Single Oak Drive
                          Temecula, CA 92590
                          E-mail: licensing@milliporesigma.com


                          5. No Products that contain this patented technology may be transferred or distributed further to third parties outside of your laboratory, unless the recipient third party receives a license from BSA and agrees to be bound by its terms.

                          By keeping or using the enclosed Product(s), you accept the above terms of use solely for noncommercial research purpose. If you refuse to accept the terms herein, you must return the Product(s) to EMD Millipore.
                          Catalogue Number71491
                          Brand Family Novagen®
                          References
                          Product Information
                          Applications
                          Biological Information
                          Physicochemical Information
                          Dimensions
                          Materials Information
                          Toxicological Information
                          Safety Information according to GHS
                          Safety Information
                          Product Usage Statements
                          Storage and Shipping Information
                          Ship Code Ambient Temperature Only
                          Toxicity Standard Handling
                          Storage +15°C to +30°C
                          Do not freeze Ok to freeze
                          Packaging Information
                          Transport Information
                          Supplemental Information
                          Specifications

                          Documentation

                          Overnight Express™ Instant TB Medium - Novagen SDS

                          Title

                          Safety Data Sheet (SDS) 

                          Overnight Express™ Instant TB Medium - Novagen Certificates of Analysis

                          TitleLot Number
                          71491

                          Brochure

                          Title
                          Bulk packaging
                          Overnight Express Autoinduction Systems

                          Citations

                          Title
                        • Erika J. Mancini, et al. (2007) Structure of the Murray Valley encephalitis virus RNA helicase at 1.9 A resolution. Protein Science 16, 2294-2300.
                        • Liguo Zhang, Kenneth P. Nephew and Patricia J. Gallagher. (2007) Regulation of death-associated protein kinase: stabilization by HSP90 heterocomplexes. Journal of Biological Chemistry 282, 11795-11804.
                        • User Protocols

                          Title
                          TB526 Overnight Express™ Instant LB and TB Media

                          Related Products & Applications

                          Related Products

                          Catalog Number Description  
                          71300 Overnight Express™ Autoinduction System 1 - Novagen Show Pricing & Availability
                          71366 Overnight Express™ Autoinduction System 2 - Novagen Show Pricing & Availability

                          Related Products By: Brand Facete

                          Categories

                          Life Science Research > Cell Culture and Systems > Cell Culture Media and Supplements > Bacterial Culture Media