Pricing & Availability
An invalid quantity was specified. The product quantity has been adjusted.
|70837-3||Plastic ampoule||0.4 ml||
|70837-4||Glass bottle||1 ml||
|Overview||Origami™ B host strains carry the same mutations
in trxB and gor as the original Origami strains,
except that they are derived from a lacZY mutant
of BL21 to enable precise control of expression
levels by adjusting the concentration of IPTG.
Thus the Origami B strains combine the desirable
characteristics of BL21, Tuner™, and Origami
strains in one strain background. The mutations
in trxB and gor are selectable on kanamycin and
tetracycline, respectively; therefore, these strains
cannot be used with plasmids that can only
selected with kanamycin or tetracycline. These
strains also include the lon and ompT deficiences
of BL21, which increase protein stability.
DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
|Genotype: F- ompT hsdSB(rB- mB-) gal dcm lacY1 ahpC (DE3) gor522::
Tn10 trxB (KanR, TetR)
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
|Guaranteed efficiency||Guaranteed efficiency: > 2 x 10⁶ cfu/μg|
|Safety Information according to GHS|
|Product Usage Statements|
|Storage and Shipping Information|
|Ship Code||Dry Ice Only|
|Toxicity||Multiple Toxicity Values, refer to MSDS|
|Do not freeze||Ok to freeze|
Origami™ B(DE3) Competent Cells - Novagen SDS
|Competent Cell Brochure|
|inNovations Newsletter 23|