70837 Origami™ B(DE3) Competent Cells - Novagen

70837
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      Overview

      Replacement Information

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      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      70837-3
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          Plastic ampoule 0.4 ml
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          70837-4
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              Glass bottle 1 ml
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              Description
              OverviewOrigami™ B host strains carry the same mutations in trxB and gor as the original Origami strains, except that they are derived from a lacZY mutant of BL21 to enable precise control of expression levels by adjusting the concentration of IPTG. Thus the Origami B strains combine the desirable characteristics of BL21, Tuner™, and Origami strains in one strain background. The mutations in trxB and gor are selectable on kanamycin and tetracycline, respectively; therefore, these strains cannot be used with plasmids that can only selected with kanamycin or tetracycline. These strains also include the lon and ompT deficiences of BL21, which increase protein stability.

              DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
              Genotype: F- ompT hsdSB(rB- mB-) gal dcm lacY1 ahpC (DE3) gor522:: Tn10 trxB (KanR, TetR)
              Catalogue Number70837
              Brand Family Novagen®
              References
              Product Information
              Components
              Guaranteed efficiencyGuaranteed efficiency: > 2 x 10⁶ cfu/μg
              Applications
              Biological Information
              Physicochemical Information
              Dimensions
              Materials Information
              Toxicological Information
              Safety Information according to GHS
              Safety Information
              Product Usage Statements
              Storage and Shipping Information
              Ship Code Dry Ice Only
              Toxicity Multiple Toxicity Values, refer to MSDS
              Storage ≤ -70°C
              Do not freeze Ok to freeze
              Packaging Information
              Transport Information
              Supplemental Information
              Specifications

              Documentation

              SDS

              Title

              Safety Data Sheet (SDS) 

              Certificates of Analysis

              TitleLot Number
              70837

              Brochure

              Title
              Competent Cell Brochure
              inNovations Newsletter 23

              Citations

              Title
            • Chikako Fukuda, Shigeyuki Kawai and Kousaku Murata. (2007) NADP(H) Phosphatase Activities of Archaeal Inositol Monophosphatase and Eubacterial 3'-Phosphoadenosine 5'-Phosphate Phosphatase. Applied and Enviornmental Microbiology 73, 5447-5452.
            • R. Jeremy Johnson, Shawn R. Lin and Ronald T. Raines. (2007) Genetic selection reveals the role of a buried, conserved polar residue. Protein Science 16, 1609-1616.
            • Gang Li, et al. (2005) N-Terminal insertion and C-terminal ankyrin-like repeats of α-latrotoxin are critical for Ca2+-dependent exocytosis. Journal of Neuroscience 25, 10188-10197.
            • HaJeung Park, Floyd G. Adsit and Jeffrey C. Boyington. (2005) The 1.4 A crystal structure of the human oxidized low density lipoprotein receptor LOX-1. Journal of Biological Chemistry 280, 13593-13599.
            • Naoko Tsuneyoshi, et al. (2005) The functional and structural properties of MD-2 required for lipopolysaccharide binding are absent in MD-11. Journal of Immunology 174, 340-344.
            • Li Wang, Gang Li and Shuzo Sugita. (2005) A central kinase domain of yype I phosphatidylinositol phosphate kinases is sufficient to prime exocytosis: isoform secificity and its underlying mechanism. Journal of Biological Chemistry 280, 16522-16527.
            • M. Claire H. Holland and John D. Lambris. (2004) A Functional C5a anaphylatoxin receptor in a teleost species. Journal of Immunology 172, 349-355.
            • M. Miertzschke and T. Greiner-Stoffele. (2003) The xthA gene product of Archaeoglobus fulgidus is an unspecific DNase. European Journal of Biochemistry 270, 1838-1849.
            • Atsushi Kodan, Hiroyuki Kuroda and Fukumi Sakai. (2002) A stilbene synthase from Japanese red pine (Pinus densiflora): Implications for phytoalexin accumulation and down-regulation of flavonoid biosynthesis. Procedings of the National Academy of Science 99, 3335-3339.
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              Categories

              Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Competent Cells for Expression