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71091 | NovaTaq™ Hot Start DNA Polymerase

71091
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      Overview

      Replacement Information

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      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      71091-3
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          Glass bottle 250 u
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          71091-4
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              Description
              OverviewNovaTaq™ Hot Start DNA Polymerase is a chemically modified form of Taq DNA polymerase that is inactive at ambient temperature. The enzyme provides improved specificity when compared to standard Taq DNA polymerase and can minimize the generation of nonspecific amplification products, such as primer-dimers and misprimed products. The enzyme must be activated by heat treatment (7–10 min at 95°C), after which thermal cycling can proceed. The enzyme generates PCR products with 3′-dA overhangs, suitable for cloning with the Novagen Perfectly Blunt®, AccepTor™, and LIC Vector Kits.



              Source Recombinant Thermus aquaticus DNA polymerase expressed in E. coli
              Concentration 5.0 U/µl
              Endonuclease None detected
              Exonuclease None detected
              Amplification effiency Functional PCR
              Storage –20°C
              Catalogue Number71091 Brand Family Novagen®
              Features and benefits
              • Higher PCR specificity and yield
              • Improved low-copy target amplification
              • Ambient temperature setup compatible with automation
              • Target amplification of up to 5 kbp
              • Ideal for quantitative and high-throughput PCR applications
              References
              Product Information
              Unit of DefinitionOne unit is defined as the amount of enzyme that will catalyze the incorporation of 10 nmol of dNTP into acid-insoluble form in 30 min at 72°C, in a reaction containing 25 mM TAPS (tris-[hydroxymethyl]-methyl-amino-propane-sulfonic acid, sodium salt), pH 9.3 at 25°C, 50 mM KCl, 2 mM MgCl₂, 1 mM 2-mercaptoethanol, 0.2 mM dATP, dGTP, and dTTP, 0.1 µM [α-³²P]dCTP, and 12.5 µg activated salmon sperm DNA in a volume of 50 µl.
              250 U or 5 × 250 UNovaTaq Hot Start DNA Polymerase (5 U/µl)
              1.5 ml or 5 × 1.5 ml10X NovaTaq Hot Start Buffer
              1.5 ml or 5 × 1.5 ml25 mM MgCl₂
              DeclarationPurchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser's own internal research. No other patents rights are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California, 94404, USA.
              Applications
              ApplicationNovaTaq™ Hot Start DNA Polymerase is a heat-activatable modified form of recombinant Taq DNA polymerase
              Biological Information
              Physicochemical Information
              Dimensions
              Materials Information
              Toxicological Information
              Safety Information according to GHS
              Safety Information
              Product Usage Statements
              Storage and Shipping Information
              Ship Code Dry Ice Only
              Toxicity Multiple Toxicity Values, refer to MSDS
              Storage -20°C
              Do not freeze Ok to freeze
              Packaging Information
              Transport Information
              Supplemental Information
              Specifications

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              Categories

              Life Science Research > Genomic Analysis > DNA Preparation & Cloning > PCR & RT-PCR Kits & Reagents