71377 NoShift™ Transcription Factor Assay Kit

71377
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      Overview

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      71377-3
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          Plastic ampoule 96 rxn
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          71377-3REF
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              Glass bottle 3 ref
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              Description
              OverviewThe NoShift™ Transcription Factor Assay Kit is a microassay plate–based method for analysis of DNA-binding proteins that alleviates many of the problems associated with gel shift assays. The gel shift, or electrophoretic mobility shift assay (EMSA), is a method for analyzing interactions between proteins and DNA. This technique, first published in 1981 (Fried 1981, Garner 1981), is based on the reduced electrophoretic mobility of protein–DNA complexes compared with that of DNA alone under nondenaturing conditions. The traditional gel shift assay is tedious and time consuming, requires radio-labeled probe, and often requires extensive optimization. The 96-well NoShift plate format is versatile and enables simultaneous analysis of multiple binding factors in less than five hours without the use of radioisotopes. A biotinylated oligonucleotide is mixed with a protein sample and the protein–DNA complex is captured on streptavidin plates. The bound transcription factor is detected with a specific antibody followed by a second antibody–HRP conjugate and chromogenic substrate.

              Each NoShift Transcription Factor Assay Kit comes with one 96-strip-well streptavidin-coated plate, binding reaction buffer, antibody dilution buffer, plate wash buffer, and TMB substrate and provides enough reagents for 100 reactions. The NoShift Kit is designed for use with oligonucleotides defining a protein binding consensus sequence provided by the purchaser and an antibody to a DNA binding protein.

              Catalogue Number71377
              Brand Family Novagen®
              References
              ReferencesFried, M. and Crothers, D.M. 1981. Nucleic Acids Res. 9, 6505. Garner, M.M. and Revzin, A. 1981. Nucleic Acids Res. 9, 3047.
              Product Information
              4 × 1 ml4X NoShift™ Bind Buffer
              150 µlSalmon Sperm DNA
              125 µlPoly(dI:dC):Poly(dI:dC)
              30 ml10X NoShift Wash Buffer
              20 mlNoShift Antibody Dilution Buffer
              1 eaStreptavidin Plate
              12 mlTMB Substrate
              100 µl100 mM DTT
              3 eaAluminum Plate Sealer
              Applications
              Biological Information
              Physicochemical Information
              Dimensions
              Materials Information
              Toxicological Information
              Safety Information according to GHS
              Safety Information
              Product Usage Statements
              Storage and Shipping Information
              Ship Code Blue Ice Only
              Toxicity Multiple Toxicity Values, refer to MSDS
              Storage Multiple storage requirements
              Do not freeze Ok to freeze
              Packaging Information
              Transport Information
              Supplemental Information
              Specifications

              Documentation

              NoShift™ Transcription Factor Assay Kit SDS

              Title

              Safety Data Sheet (SDS) 

              NoShift™ Transcription Factor Assay Kit Certificates of Analysis

              TitleLot Number
              71377

              References

              Reference overview
              Fried, M. and Crothers, D.M. 1981. Nucleic Acids Res. 9, 6505. Garner, M.M. and Revzin, A. 1981. Nucleic Acids Res. 9, 3047.

              Citations

              Title
            • Markus Bredel, et al. (2006) Tumor necrosis factor-α-induced protein 3 as a putative regulator of nuclear factor-κB-mediated resistance to O6-alkylating agents in human glioblastomas. Journal of Clinical Oncology 24, 274-287.
            • Zachary T. Resch, et al. (2005) Stress-activated signaling pathways mediate the stimulation of pregnancy-associated plasma protein-A expression in cultured human fibroblasts. Endocrinology 147, 885-890.
            • Andrei P. Pomerantsev, Olga M. Pomerantsev and Stephen H. Leppla. (2004) A spontaneous translational fusion of Bacillus cereus plcR and papR activates transcription of plcR-dependent genes in Bacillus anthracis via binding with a specific pallindromic sequence. Infection and Immunity 72, 5814-5823.
            • User Protocols

              Title
              TB403 NoShift™ Transcription Factor Assay Kit