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481909 | Niclosamide - CAS 50-65-7 - Calbiochem

481909
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      Overview

      Replacement Information

      Key Specifications Table

      Empirical FormulaCAS #
      C₁₃H₈Cl₂N₂O₄ 50-65-7

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      481909-1GM
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      Available
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          Glass bottle 1 gm
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          Description
          OverviewA cell-permeable salicylanilide that, in addition to its well-known antihelmintic efficacy, acts as a mammalian mTORC1, but not mTORC2, signaling inhibitor mechanistically distinct from rapamycin (Cat. Nos. 553210, 553211, & 553212). Likely a direct consequence of autophagy activation, Niclosamide is demonstrated to induce Wnt-independent Frizzled1 and Dishevelled-2 downregulation. Unrelated to its autophagy induction activity, Niclosamide is also shown to inhibit Stat3 signaling (IC50 = 0.25 µM in HeLa reporter assays). Efficiently inhibits breast cancer stem-like cells in vitro and in vivo.
          Catalogue Number481909
          Brand Family Calbiochem®
          Synonyms5-Chloro-N-(2-chloro-4-nitrophenyl)-2-hydroxy-benzamide, mTOR Inhibitor IX, Autophagy Inducer III, STAT3 Inhibitor XV, Wnt Pathway Inhibitor XIII
          References
          ReferencesWang, Y.C., et al. 2013. PLoS ONE 8, e74538.
          Chen, W., et al. 2010. Am. J. Physiol. Gastrointest. Liver Physiol. 299, G293.
          Gies, E., et al. 2010. PLoS ONE 5, e14410.
          Ren, X., et al. 2010. ACS Med. Chem. Lett. 1, 454.
          Chen, M., et al. 2009. Biochemistry 48, 10267.
          Product Information
          CAS number50-65-7
          FormPale yellow solid
          Hill FormulaC₁₃H₈Cl₂N₂O₄
          Chemical formulaC₁₃H₈Cl₂N₂O₄
          Structure formula Image
          Applications
          Biological Information
          Purity≥97% by HPLC
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
          Toxicity Regulatory Review
          Storage +2°C to +8°C
          Protect from Light Protect from light
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C. Stock solutions are stable for up to 6 months at -20°C.
          Packaging Information
          Packaged under inert gas Packaged under inert gas
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          481909

          References

          Reference overview
          Wang, Y.C., et al. 2013. PLoS ONE 8, e74538.
          Chen, W., et al. 2010. Am. J. Physiol. Gastrointest. Liver Physiol. 299, G293.
          Gies, E., et al. 2010. PLoS ONE 5, e14410.
          Ren, X., et al. 2010. ACS Med. Chem. Lett. 1, 454.
          Chen, M., et al. 2009. Biochemistry 48, 10267.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision26-March-2012 JSW
          Synonyms5-Chloro-N-(2-chloro-4-nitrophenyl)-2-hydroxy-benzamide, mTOR Inhibitor IX, Autophagy Inducer III, STAT3 Inhibitor XV, Wnt Pathway Inhibitor XIII
          DescriptionA cell-permeable salicylanilide that, in addition to its well-known antihelmintic efficacy and use in tapeworm treatment, acts as a mammalian mTORC1, but not mTORC2, signaling inhibitor mechanistically distinct from rapamycin (Cat. Nos. 553210, 553211, & 553212), causing MCF-7 autophagy activation under nutrient-rich conditions (by 15-fold with 4h 10 µM treatment), SH-SY5Y lysosome redistribution (10 µM for 8 h), and dissipation/clearance of ubiquinated protein aggregates upon proteasome inhibition in a reversible, time- and dose-dependent manner. Likely a direct consequence of autophagy activation, Niclosamide is demonstrated to induce Wnt-independent Frizzled1 endocytosis (t1/2 = 2.4 h; effective conc. <2 µM) and Dishevelled-2 downregulation (IC50 ~1 µM), effectively preventing Wnt3a from stimulating HEK293 cellular LEF/TCF transcription activity (IC50 = 0.5 µM TOPFlash reporter assays). Unrelated to its autophagy induction activity, Niclosamide is also shown to inhibit Stat3 signaling (IC50 = 0.25 µM in HeLa-based pLucTKS3 reporter assays) by suppressing the phosphorylation of Jak1 Tyr1022/1023 (2 h treatment at 0.5 to 2.0 µM in Du145 cultures) and Stat3 Tyr705 (by >90% after 24 h treatment at 2 and 5 µM, respectively, in Du145 and HeLa cultures), without affecting the phosphorylation of Stat1 Tyr701, Stat3 Ser727, Stat5 Tyr694, or Jak2 Tyr1007/1008.
          FormPale yellow solid
          Intert gas (Yes/No) Packaged under inert gas
          CAS number50-65-7
          Chemical formulaC₁₃H₈Cl₂N₂O₄
          Structure formula
          Purity≥97% by HPLC
          SolubilityDMSO (2.5 mg/ml) or Ethanol (5 mg/ml)
          Storage +2°C to +8°C
          Protect from light
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C. Stock solutions are stable for up to 6 months at -20°C.
          Toxicity Regulatory Review
          ReferencesWang, Y.C., et al. 2013. PLoS ONE 8, e74538.
          Chen, W., et al. 2010. Am. J. Physiol. Gastrointest. Liver Physiol. 299, G293.
          Gies, E., et al. 2010. PLoS ONE 5, e14410.
          Ren, X., et al. 2010. ACS Med. Chem. Lett. 1, 454.
          Chen, M., et al. 2009. Biochemistry 48, 10267.