NS230 Neurite Outgrowth Assay Plus Kit (3 µm)

12 assays  
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      Key Specifications Table

      Key ApplicationsDetection Methods
      Neuroscience, Neurotoxicity Chemiluminescence
      Catalogue NumberNS230
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionNeurite Outgrowth Assay Plus Kit (3 µm)

      Millipore’s NS230 Neurite Outgrowth Assay Plus Kit (3 µm) utilizes microporous tissue culture insert technology from Millipore and is based on the use of Millicell inserts which contain a permeable membrane with 3-μm pores. The Millicell inserts utilized in this kit are appropriate for cells projecting neurites of up to 3 µm in diameter, e.g. N1E-115 cells [5], Dorsal Root Ganglia [6], and Schwann cells [6]. The inserts are designed to fit into standard 12 well tissue culture plates, which when in use contain differentiation media in contact with the bottom of the insert membrane. The permeable membranes allow for discrimination between neurites and cell bodies, as projecting neurites will pass easily through the pores but cell bodies will not. Therefore, by inducing neurites to traverse these membrane pores, a purified population of neurites is located on the underside of the membrane, distal to the surface on which neural cell bodies are deposited.
      The assay is a simple, efficient, and versatile system for the quantitative determination of compounds that influence neurite formation and repulsion. With this system, it is possible to screen numerous biological and pharmacological agents simultaneously, directly evaluate adhesion and guidance receptor functions responsible for neurite extension and repulsion, as well as analyze gene function in transfected cells. The microporous filter also allows for biochemical separation and purification of neurites and cell bodies for detailed molecular analysis of protein expression, signal transduction processes, and identification of drug targets that regulate neurite outgrowth or retraction processes. Reagents and materials supplied in the NS230 Neurite Outgrowth Assay Plus kit are sufficient for testing neurite outgrowth in 12 separate assay wells.
      Note: the inserts in this kit contain 3 µm pores and are unsuitable for use with certain small diameter cells like PC12 [5]. Small sized cells have the potential to pass through the pores in the membrane and can obscure the results. Millipore’s NS255 Neurite Outgrowth Assay Plus kit (1 µm) has been specifically designed and validated for small diameter cells such as PC12s.
      Materials Required but Not Delivered1. Tissue culture instruments/supplies (37ºC incubator, growth media, flasks/plates, sterile TC grade PBS, Sodium Pyruvate, etc.)
      2. Dulbecco's Modified Eagle Media 1X, Liquid: (Part No. SLM-020-A)
      3. EmbryoMax® ES Cell Qualified Penicillin-Streptomycin Solution,100X: (Part No. TMS-AB2-C)
      4. EmbryoMax® ES Cell Qualified L-Glutamine Solution,100X (200mM): (Part No. TMS-002-C)
      5. Methanol
      6. 12-well tissue culture plates
      7. Milli-Q™ water
      8. Spectrophotometer
      9. Protein lysis buffer and/or fluorescently labeled secondary antibody (for optional protocols)
      Product Information
      • NS230-1 (Store at 2-8ºC)
        1. Neurite Outgrowth Plate Assembly, 3 µm: (Part No. CS202680) One 12 well tissue culture plate containing 12 Millicell inserts with 3 µm pore size membranes.
        2. Neurite Outgrowth Plate: (Part No. CS203338) One extra 12 well tissue culture plate.
        3. 10X Wash Buffer: (Part No. CS201873) One 130 mL bottle.
        4. Goat anti-Mouse IgG, (H+L) HRP Conjugate: (Part No.CS203339) 10 µL of antibody solution.
        5. 10X Cell Dissociation Buffer: (Part No. CS202683) One 10mL bottle.
        6. 10X Stain Solution: (Part No. CS203337) One 1 mL vial.
        7. TMB Solution: (Part No. CS202682) One 10 mL bottle.
        8. Stop Solution: (Part No. CS203340) One 1.8 mL vial.
        9. Cotton Swabs: (Part No. 10202) One pack of 50.
        NS230-2 (Store at -20ºC)
        1. Laminin: (Part No. CS202685) 60 µL of 1 mg/mL solution.
        2. BSA Solution: (Part No. CS202686) 30 µL of 100 mg/mL solution.
        3. 5X Blocking Buffer: (Part No. CS203336) One 6 mL bottle.
        4. Anti-Tubulin, βIII Antibody: (Part No. CS202681) 15 µL of antibody solution.
      Detection methodChemiluminescence
      ApplicationThis NS230 Neurite Outgrowth Assay Plus Kit (3 µm) utilizes microporous tissue culture insert technology from Millipore & is based on the use of Millicell inserts which contain a permeable membrane with 3-μm pores.
      Key Applications
      • Neuroscience
      • Neurotoxicity
      Biological Information
      Species Reactivity
      • All
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsWhen stored properly the kit components are stable for 3 months from date of receipt. Anti-Tubulin, β III antibody, laminin, BSA solution, and 5X Blocking Buffer should be stored at -20ºC (NS230-2 reagents) whereas the remaining components (NS230-1 reagents) can be kept at 4ºC. Discard any remaining reagents after the expiration date.
      Packaging Information
      Material Size12 assays
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 


      Reference overviewPub Med ID
      Identification of process-localized mRNAs from cultured rodent hippocampal neurons.
      Poon, Michael M, et al.
      J. Neurosci., 26: 13390-9 (2006)  2006

      Show Abstract
      17182790 17182790


      AXIS: Axon Investigation System
      Pathways and Biomarkers of Glutamatergic Synapse Flyer (EMD)

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      Neurite Outgrowth Assay Plus Kit (3 μm )

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      Life Science Research > Cell Analysis > Cell-based Assays > Neurite Outgrowth