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NS220 | Neurite Outgrowth Assay Kit (3 µm)

12 assays  
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      Key Specifications Table

      Key ApplicationsDetection Methods
      Neuroscience Chromogenic
      Catalogue NumberNS220
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionNeurite Outgrowth Assay Kit (3 µm)

      CHEMICON's NS220 Neurite Outgrowth Assay Kit (3 µm) utilizes microporous tissue culture insert technology from Millipore and is based on the use of Millicell cell culture inserts (chambers) containing a permeable membrane with 3-μm pores at the base. The Millicell inserts utilized in this kit are appropriate for cells projecting neurites of up to 3 µm diameter, e.g. N1E-115 cells [Abe et al., 2003], Dorsal Root Ganglia and Schwann cells [Windebank et al., 1985]. The inserts are designed to fit into a receiver vessel, which when in use contains differentiation media in contact with the bottom of the insert membrane. The permeable membranes allow for discrimination between neurites and cell bodies, as projecting neurites will pass easily through the pores but cell bodies will not. Therefore, by inducing neurites to traverse these membrane pores, a purified population of neurites is located on the underside of the membrane, distal to the surface on which neural cell bodies are deposited.
      The assay is a simple, efficient, and versatile system for the quantitative determination of compounds that influence neurite formation and repulsion. With this system, it is possible to screen numerous biological and pharmacological agents simultaneously, directly evaluate adhesion and guidance receptor functions responsible for neurite extension and repulsion, as well as analyze gene function in transfected cells. The microporous filter allows for biochemical separation and purification of neurites and cell bodies for detailed molecular analysis of protein expression, signal transduction processes and identification of drug targets that regulate neurite outgrowth or retraction processes. Reagents and materials supplied in the NS220 Neurite Outgrowth Assay Kit are sufficient for 12 tests.
      Note: the 3 µm pore size inserts in this kit are unsuitable for use with PC12 cells. Due to the relatively small diameter of PC12 cell bodies [Abe et al., 2003], some of these cells may pass though 3 µm pores and obscure the results. Chemicon's NS225 Neurite Outgrowth Assay Kit (1 µm) has been specifically designed for and validated with PC12 cells.
      Materials Required but Not Delivered1. Desired extracellular matrix membrane coating protein, e.g., bovine collagen I or mouse laminin.
      2. Tissue culture instruments/supplies (including 37°C incubator, growth media, flasks/plates, detachment buffer, etc.) for cell type of interest.
      3. Differentiation media/factors for cell type of interest (e.g., nerve growth factor, serum-free media).
      4. 1X phosphate buffered saline (PBS) with Ca2+/Mg2+.
      5. Inverted microscope for neurite visualization on membrane inserts.
      6. Parafilm and spectrophotometer for neurite stain extraction and quantification.
      7. Protein lysis buffer (optional)
      Product Information
      • 1. Neurite Outgrowth Plate Assembly, 3 μm: (Part No. 2007256) 1 x 24-well plate containing 12 x Millicell hanging inserts with 3 μm pore size membranes.
      • 2. Neurite Stain Solution: (Part No. 90242) One 20 mL bottle.
      • 3. Neurite Stain Extraction Buffer: (Part No. 90243) One 20 mL bottle.
      • 4. Neurite Outgrowth Assay Plate: (Part No. 2007255) Two 24-well plates.
      • 5. Cotton Swabs: (Part No. 10202) 50 swabs.
      • 6. Forceps: (Part No. 10203) One each.
      Detection methodChromogenic
      ApplicationThe NS220 Neurite Outgrowth Assay Kit (3 µm) is based on the use of Millicell cell culture inserts (chambers) containing a permeable membrane with 3-μm pores at the base.
      Key Applications
      • Neuroscience
      Biological Information
      Species Reactivity
      • All
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsWhen stored at 2-8° C, the kit components are stable up to the expiration date. Do not freeze or expose to elevated temperatures. Discard any remaining reagents after the expiration date.
      Packaging Information
      Material Size12 assays
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 


      Reference overviewPub Med ID
      Co-administration of ciliary neurotrophic factor with its soluble receptor protects against neuronal death and enhances neurite outgrowth.
      Ozog, Mark A, et al.
      J. Biol. Chem., 283: 6546-60 (2008) 2008

      18086669 18086669
      Identification of process-localized mRNAs from cultured rodent hippocampal neurons.
      Poon, Michael M, et al.
      J. Neurosci., 26: 13390-9 (2006) 2006

      Show Abstract
      17182790 17182790
      Small GTPase Tc10 and its homologue RhoT induce N-WASP-mediated long process formation and neurite outgrowth.
      Abe, Tomoyuki, et al.
      J. Cell. Sci., 116: 155-68 (2003) 2003

      Show Abstract
      12456725 12456725
      Spinal cord repair: strategies to promote axon regeneration.
      McKerracher, L
      Neurobiol. Dis., 8: 11-8 (2001) 2001

      Show Abstract
      11162236 11162236
      Repulsive factors and axon regeneration in the CNS
      Fournier, A E and Strittmatter, S M
      Curr Opin Neurobiol, 11:89-94 (2001) 2001

      11179877 11179877
      Glial inhibition of nerve regeneration in the mature mammalian CNS.
      Qiu, J, et al.
      Glia, 29: 166-74 (2000) 2000

      Show Abstract
      10625335 10625335
      Axon regeneration: Vaccinating against spinal cord injury
      Filbin, M T
      Curr Biol, 10:R100-R103 (2000) 2000

      10679313 10679313
      Myelination determines the caliber of dorsal root ganglion neurons in culture
      Windebank, A J, et al
      J Neurosci, 5:1563-1569 (1985) 1985

      4009246 4009246


      AXIS: Axon Investigation System


      Poster: High Throughput Screening To Identify Novel Molecular Pathways Involved In Neurite Outgrowth

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      Neurite Outgrowth Assay Kit

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      Life Science Research > Cell Analysis > Cell-based Assays > Neurite Outgrowth