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492027 | NRG1 Isoform SMDF, Human, Recombinant

492027
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      Overview

      Replacement Information

      Key Specifications Table

      Purity
      >95% by SDS-PAGE

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      492027-25UG
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      Limited AvailabilityLimited Availability
      Stocked 
      Discontinued
      Limited Quantities Available
      Available
        Remaining : Will advise
          Remaining : Will advise
          Will advise
          Contact Customer Service

          Glass bottle 25 μg
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          Description
          OverviewRecombinant, human neuregulin 1 (NRG1), isoform SMDF (sensory and motor neuron-derived factor), consisting of amino acids 1-263, 117-296, and 162-296, each fused at the C-terminus to a His•Tag® sequence and expressed in S. frugiperda Sf21 cells using a baculovirus expression system. The neuregulin (also known as heregulin) family of cytokines is comprised of multiple secreted or membrane-bound isorforms that are produced from the single heregulin gene by alternative splicing and/or usage of different promoters. NRG1 isoform SMDF is a type III isoform with a C-terminal EGF-like domain that interacts with the erbB family of tyrosine kinase receptors. NRG1 isoform SMDF expression has been found to be restricted to the nervous system and is believed to play an important role in the development of the nervous system.
          Catalogue Number492027
          Brand Family Calbiochem®
          SynonymsHeregulin-1
          References
          ReferencesMeyer, D. et al. 1997. Development 124, 3575.
          Yarden, Y. and D. Wen 1994. in Guidebook to Cytokinesand Their Receptors, N.A. Nicola, Ed., Oxford University Press, p. 146.
          Karey, K.P., et al. 1988. Cancer Res. 48, 4083.
          Product Information
          FormLyophilized
          FormulationLyophilized from 0.2 µm filtered PBS, BSA.
          Applications
          Biological Information
          Biological activityED₅₀ = 1-5 ng/ml as measured in a serum-free proliferation assay using MCF-7 human breast adenocarcinoma cells.
          Purity>95% by SDS-PAGE
          Physicochemical Information
          ContaminantsEndotoxin: ≤1.0 EU/µg protein
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage -20°C
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstituion refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Certificates of Analysis

          TitleLot Number
          492027

          References

          Reference overview
          Meyer, D. et al. 1997. Development 124, 3575.
          Yarden, Y. and D. Wen 1994. in Guidebook to Cytokinesand Their Receptors, N.A. Nicola, Ed., Oxford University Press, p. 146.
          Karey, K.P., et al. 1988. Cancer Res. 48, 4083.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision22-June-2011 RFH
          SynonymsHeregulin-1
          DescriptionRecombinant, human neuregulin 1 (NRG1), isoform SMDF (sensory and motor neuron-derived factor), consisting of amino acids 1-263, 117-296, and 162-296, each fused at the C-terminus to a His•Tag® sequence and expressed in S. frugiperda Sf21 cells using a baculovirus expression system. The neuregulin (also known as heregulin) family of cytokines is comprised of multiple secreted or membrane-bound isorforms that are produced from the single heregulin gene by alternative splicing and/or usage of different promoters. NRG1 isoform SMDF is a type III isoform with a C-terminal EGF-like domain that interacts with the erbB family of tyrosine kinase receptors. NRG1 isoform SMDF expression has been found to be restricted to the nervous system and is believed to play an important role in the development of the nervous system.
          FormLyophilized
          FormulationLyophilized from 0.2 µm filtered PBS, BSA.
          Purity>95% by SDS-PAGE
          ContaminantsEndotoxin: ≤1.0 EU/µg protein
          Biological activityED₅₀ = 1-5 ng/ml as measured in a serum-free proliferation assay using MCF-7 human breast adenocarcinoma cells.
          SolubilityReconstitute to 100 µg/ml with sterile PBS containing ≥1% human or bovine serum albumin.
          Storage -20°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstituion refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
          Toxicity Standard Handling
          ReferencesMeyer, D. et al. 1997. Development 124, 3575.
          Yarden, Y. and D. Wen 1994. in Guidebook to Cytokinesand Their Receptors, N.A. Nicola, Ed., Oxford University Press, p. 146.
          Karey, K.P., et al. 1988. Cancer Res. 48, 4083.