481418 | NEMO NOA Peptide, Cell-Permeable, A-UBI - Calbiochem

481418
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      Overview

      Replacement Information

      Key Specifications Table

      Empirical Formula
      C₂₁₅H₃₄₇N₇₁O₅₀S₁ plus TFA and water

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      481418-2MG
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          Glass bottle 2 mg
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          Description
          OverviewA cell-permeable 37-mer peptide derived from the NEMO NOA ubiquitin binding site (294-314 of mouse sequence with D304R), and fused with an Antennapedia cell penetrating protein (A) at the N-terminus. It is shown to attenuate NF-κB activation via blocking interactions between K63-polyubiquitin chains and the NOA ubiquitin binding domain of NEMO (Kd = 17 µM for CC2-LZ/A-UBI complex). The biotin-labled A-UBI specifically inhibits the LPS-induced NF-κB activation (IC50 = 1.1 µM, compared with 23 µM for IKKβ derived A-NBD) and TNF-α induced NF-κB activation (IC50 = 21 µM) in 70Z/3-C3 pre-B lymphocytes. It displays an inhibitory effect on nuclear translocation of NF-κB p65 and IKK kinase activity at 10 µM, but does not significantly affect other signaling pathways such as MAPK/ERK, MAPK/JNK, NF-κB and NFAT.
          Catalogue Number481418
          Brand Family Calbiochem®
          SynonymsNEMO Ubiquitin Binding Domain Inhibitor
          References
          ReferencesChiaravalli, J., et al. 2011. Biochem. Pharm. 82, 1163.
          Product Information
          FormWhite powder
          Hill FormulaC₂₁₅H₃₄₇N₇₁O₅₀S₁ plus TFA and water
          Chemical formulaC₂₁₅H₃₄₇N₇₁O₅₀S₁ plus TFA and water
          Applications
          Biological Information
          Purity≥95% by HPLC
          Physicochemical Information
          Peptide SequenceRQIKIWFQNRRMKWKKLKAQADIYKARFQAERHAREK
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Shipped with Blue Ice or with Dry Ice
          Toxicity Standard Handling
          Storage -20°C
          Protect from Light Protect from light
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
          Packaging Information
          Packaged under inert gas Packaged under inert gas
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          481418

          References

          Reference overview
          Chiaravalli, J., et al. 2011. Biochem. Pharm. 82, 1163.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision10-August-2012 JSW
          SynonymsNEMO Ubiquitin Binding Domain Inhibitor
          DescriptionA cell-permeable 37-mer peptide derived from the NEMO NOA ubiquitin binding site (294-314 of mouse sequence with D304R), and fused with an Antennapedia cell penetrating protein (A) at the N-terminus. It is shown to attenuate NF-κB activation via blocking interactions between K63-polyubiquitin chains and the NOA ubiquitin binding domain of NEMO (Kd = 17 µM for CC2-LZ/A-UBI complex). The biotin-labled A-UBI specifically inhibits the LPS-induced NF-κB activation (IC50 = 1.1 µM, compared with 23 µM for IKKβ derived A-NBD) and TNF-α induced NF-κB activation (IC50 = 21 µM) in 70Z/3-C3 pre-B lymphocytes. It displays an inhibitory effect on nuclear translocation of NF-κB p65 and IKK kinase activity at 10 µM, but does not significantly affect other signaling pathways such as MAPK/ERK, MAPK/JNK, NF-κB and NFAT.
          FormWhite powder
          Intert gas (Yes/No) Packaged under inert gas
          Chemical formulaC₂₁₅H₃₄₇N₇₁O₅₀S₁ plus TFA and water
          Peptide SequenceRQIKIWFQNRRMKWKKLKAQADIYKARFQAERHAREK
          Purity≥95% by HPLC
          SolubilityH₂O (50 mg/ml)
          Storage -20°C
          Protect from light
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
          Toxicity Standard Handling
          ReferencesChiaravalli, J., et al. 2011. Biochem. Pharm. 82, 1163.