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08-145 | NEDD8 Protein

100 µg  
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      Replacement Information
      Catalogue Number08-145
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionNEDD8 Protein
      OverviewNEDD8 protein is a recombinant protein produced in E. coli corresponding to the human sequence encompassing an N-terminal His6-tag within a leader sequence. NEDD8, ubiquitin-like molecule, shares ~60% identity with ubiquitin at the amino acid level that can be covalently conjugated (neddylation) to a limited number of cellular proteins in a fashion similar to that of ubiquitin. The C-terminus of pro-NEDD8 is efficiently processed to expose glycine76 in the mature form. This processing is required for conjugation to target proteins via a range of specific activating and conjugating enzymes.
      Product Information
      Presentation100 µg of concentration 0.5 mg/mL in 20mM HEPES, pH8.0, 50mM NaCl, 1mM DTT, 10% glycerol.
      Biological Information
      Concentration0.5 mg/mL
      Entrez Gene Number
      Gene Symbol
      • NEDD8
      • MGC125897
      • Neddylin
      • Nedd-8
      • MGC125896
      • FLJ43224
      • MGC104393
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q15843 # Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C- APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.
      SIZE: 81 amino acids; 9072 Da
      SUBUNIT: Directly interacts with NUB1 and AHR. Covalently attached to cullins and p53.
      SUBCELLULAR LOCATION: Nucleus. Note=Mainly nuclear.
      TISSUE SPECIFICITY: Highly expressed in heart, skeletal muscle, spleen, thymus, prostate, testis, ovary, colon and leukocytes.
      PTM: Cleavage of precursor form by UCHL3 or SENP8 is necessary for function.
      SIMILARITY: SwissProt: Q15843 ## Belongs to the ubiquitin family.
      Molecular Weight14 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -80°C from date of shipment for up to 12 months. Avoid multiple freeze/thaw cycles. After initial defrost, aliquot into individual tubes and refreeze at -80ºC.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information


      Certificates of Analysis

      TitleLot Number
      NEDD8 Protein - 0611045522 0611045522


      Reference overviewPub Med ID
      pVHL modification by NEDD8 is required for fibronectin matrix assembly and suppression of tumor development
      Stickle, Natalie H, et al
      Mol Cell Biol, 24:3251-61 (2004) 2004

      15060148 15060148
      Regulation of cullin-based ubiquitin ligases by the Nedd8/RUB ubiquitin-like proteins.
      Parry, Geraint and Estelle, Mark
      Semin. Cell Dev. Biol., 15: 221-9 (2004) 2004

      Show Abstract
      15209382 15209382
      Neddylating the guardian; Mdm2 catalyzed conjugation of Nedd8 to p53
      Harper, J Wade
      Cell, 118:2-4 (2004) 2004

      15242638 15242638