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SF-875 | NANOG; Human, Cyanine 5, RNA Detection Probe | SmartFlare

250 reactions  
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      Replacement Information

      Key Specifications Table

      Entrez Gene NumberSpecies Reactivity
      NM_024865.2 H
      Catalogue NumberSF-875
      Trade Name
      • SmartFlare
      DescriptionNANOG; Human, Cyanine 5, RNA Detection Probe | SmartFlare
      OverviewSmartFlare is a fluorescent reagent that when added to live cells enables sequence-specific mRNA and microRNA's expression detection at single cell resolution – which can reveal new insights that lysed methods giving bulk average measurements may hide. The probes do not harm the live cells allowing researchers to not only identify cells expressing biomarkers of interest, but also enrich and sort cells based on individual expression levels through FACS. Additionally, treatment of cells with SmartFlare is compatible with downstream functional analysis, especially important for preserving precious samples.
      Background InformationWith SmartFlare™ RNA Detection probes, you can detect RNA in LIVE CELLS with ease. With just an overnight incubation of NANOG; Human, Cyanine 5, RNA Detection Probe you can detect the presence of NANOG within your cells.
      Nanog is a newly identified homeodomain gene product that directs propagation of undifferentiated embryonic stem (ES) cells and was named after the mythological Celtic land of the eternal youth, Tir Na Nog. Nanog is present in both mouse and human embryonic stem cells, and lacking in differentiated cells. It is restricted to founder cells from which ES cells can be derived. Nanog is important for maintaining the self-renewing properties of mouse embryonic stem cells and is expressed in the cells of the early embryo that are used to derive embryonic stem cells. Current research suggests that Nanog acts independently of another signal transduction pathway important to maintaining the pluripotency of stem cells, which involves the activation of Stat3.
      Product Information
      FormatCyanine 5
      ApplicationNANOG can be assayed in live cells using SmartFlare probes. The fluorescent reporter release can be detected by microscopy, flow cytometry, and FACS if enrichment of live cells based on NANOG levels is desired.
      Key Applications
      • RNA Detection
      Biological Information
      Species Reactivity
      • Human
      Entrez Gene Number
      Gene Symbol
      • NANOG
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceSmartFlare Probes have been tested in a buffer system to detect the release of the fluorophore in the presence of a complimentary base pair sequence for each lot to confirm target specificity. SmartFlare Probes have also been tested in a cell model system and demonstrated increased fluorescence in cells expressing the target compared to a negative SmartFlare Scramble Control.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at 2–8°C in lyophilized format ONLY. Room temperature is required for reconstituted product.
      Packaging Information
      Material Size250 reactions
      Transport Information
      Supplemental Information



      SmartFlare™ protocol for human embryonic stem cells
      SmartFlare™ protocol for mouse embryonic stem cells



      Safety Data Sheet (SDS) 


      Reference overviewPub Med ID
      mRNA detection in living cells: A next generation cancer stem cell identification technique.
      McClellan, S; Slamecka, J; Howze, P; Thompson, L; Finan, M; Rocconi, R; Owen, L
      Methods (San Diego, Calif.) 47-54 2015

      Show Abstract
      25920950 25920950


      Live Cell Imaging with SmartFlare™ Probes (EMD)

      Technical Info

      SmartFlare™ RNA Detection Probes: Principles, protocols and troubleshooting (EMD)

      Data Sheet

      NANOG Hu-Cyanine 5 SmartFlare™ RNA Probe

      User Guides

      SmartFlare™ Probes User Guide