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362180 | N-Glycosidase A, Almond

362180
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      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      362180-5MIU
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      Stocked 
      Discontinued
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      Available
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          Glass bottle 5 miu
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          Description
          OverviewNative N-Glycosidase A from almonds. Cleaves N-glycan chains from glycopeptides, including those with α1,3-linked core fucose. This enzyme cleaves between GlcNAc and asparagine.
          Note: 1 mU = 1 milliunit.
          Catalogue Number362180
          Brand Family Calbiochem®
          SynonymsGlycopeptidase A, PNGase A
          References
          ReferencesMeans, R.E. and Desrosiers, R.C. 2000. J. Virol. 74, 11181.
          Fan, J.Q., and Lee, Y.C. 1997. J. Biol. Chem. 272, 27058.
          Altmann, F., et al. 1995. Glycoconj. J. 12, 84.
          Takahashi, N., and Nishibe, H. 1978. J. Biochem. 84, 1467.
          Product Information
          CAS number83534-39-8
          Unit of DefinitionOne unit is defined as the amount of enzyme that will catalyze the release of 1.0 µmol hybrid and high-mannose oligosaccharides from ovalbumin glycopeptide per min at 37°C, pH 5.0.
          EC number3.5.1.52
          FormLiquid
          FormulationIn 50 mM citrate/phosphate buffer, 50% glycerol, pH 5.0.
          Applications
          Biological Information
          Specific Activity≥500 mU/mg protein
          Physicochemical Information
          Contaminantsα- and β-galactosidase, β-glucosidase: ≤0.1%; proteases: none detected
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          362180

          References

          Reference overview
          Means, R.E. and Desrosiers, R.C. 2000. J. Virol. 74, 11181.
          Fan, J.Q., and Lee, Y.C. 1997. J. Biol. Chem. 272, 27058.
          Altmann, F., et al. 1995. Glycoconj. J. 12, 84.
          Takahashi, N., and Nishibe, H. 1978. J. Biochem. 84, 1467.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision13-September-2007 RFH
          SynonymsGlycopeptidase A, PNGase A
          DescriptionNative N-Glycosidase A from almonds. Cleaves N-glycan chains from glycopeptides, including those with α1,3-linked core fucose. This enzyme cleaves between GlcNAc and asparagine. Has an optimal pH of 4.0-6.0.
          FormLiquid
          FormulationIn 50 mM citrate/phosphate buffer, 50% glycerol, pH 5.0.
          Recommended reaction conditions
          Deglycosylation of Glycopeptides For assaying N-Glycosidase A Use 0.5 mU/ml N-Glycosidase A in 100 mM citrate / phosphate buffer, pH 5.0 with 0.1% BSA. Guideline used for unit definition: 10 µl of 100 µM ovalbumin glycopeptide was incubated with 10 µl 0.5 mU/ml N-Glycosidase A at 37°C for 60 min. Reaction products were quantified by absorption at 220 nm on reversed phase HPLC (5 µm ODS-Hypersil column) at 220 nm. For preparative digestion Incubate 0.2-0.5 mU N-Glycosidase A with 100 nmoles glycopeptide in 20-50 µl citrate/phosphate buffer without BSA for 24 hrs at 37°C. Deglycosylation of Glycoproteins Various reagents such as 0.75 M β-mercaptethanol, 2% Triton X-100, 2% Tween®-80 detergent may be required to denature substrates sufficiently for deglycosylation. Enzyme activity may be significantly increased by addition of Mg2+, Zn2+, Co3+, or Cu3+. A guideline is 4 µglycoprotein incubated with 1 mU N-Glycosidase A in 10 mM sodium acetate, 0.5 M NaSCN, 0.1 M β-mercaptoethanol, pH 5.1, for 24 h at 37°C. However the nature of the glycoprotein substrate will determine optimal conditions. References Taga, E.M. et al. 1984. Biochemistry 23, 815. Tarentino, A.L. and Plummer, T.H. 1982. J. Biol. Chem. 257, 10776. Takahashi, N. and Nishibe, H. 1981. Biochim. Biophys. Acta 657, 457
          CAS number83534-39-8
          EC number3.5.1.52
          Contaminantsα- and β-galactosidase, β-glucosidase: ≤0.1%; proteases: none detected
          Specific activity≥500 mU/mg protein
          Unit definitionOne unit is defined as the amount of enzyme that will catalyze the release of 1.0 µmol hybrid and high-mannose oligosaccharides from ovalbumin glycopeptide per min at 37°C, pH 5.0.
          Storage -20°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          ReferencesMeans, R.E. and Desrosiers, R.C. 2000. J. Virol. 74, 11181.
          Fan, J.Q., and Lee, Y.C. 1997. J. Biol. Chem. 272, 27058.
          Altmann, F., et al. 1995. Glycoconj. J. 12, 84.
          Takahashi, N., and Nishibe, H. 1978. J. Biochem. 84, 1467.