MCH200107 Muse™Multi-Color DNA Damage Kit

MCH200107
50 Tests  
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      Overview

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      Description
      Catalogue NumberMCH200107
      Trade Name
      • Muse
      DescriptionMuse™Multi-Color DNA Damage Kit
      OverviewEMD Millipore’s Muse™ Multi-Color DNA Damage kit is designed to enable researchers a quick and easy way to detect the activation of ATM and H2A.X using the Muse Cell Analyzer. The kit was designed and optimized using a DNA damaging reagent, Etoposide, on HeLa cells as a model system. But the kit can be used with other human cell lines to investigate both the physical and chemical factors which can induce the DNA damage response through the ATM dependent signaling pathway. Data generated using the Muse™ Cell Analyzer along with the corresponding Muse™ software module provides statistical values measuring:

      • Percentage of negative cells (e.g. no DNA damage)
      • Percentage of ATM activated cells
      • Percentage of H2A.X activated cells
      • Percentage of DNA double-strand breaks (dual activation of both ATM and H2A.X)

      The Muse™ Multi-Color DNA Damage Kit includes two directly conjugated antibodies, a phospho-specific ATM (Ser1981)-PE and a phospho-specific Histone H2A.X-PECy5 conjugated antibody to measure the extent of DNA damage in testing samples. This two color kit is designed to detect the phosphorylation state of ATM and Histone H2A.X simultaneously by flow analysis. Together, two color analysis of ATM and Histone H2A.X activation in multiplex provides a more reliable detection method for assessing the DNA damage signaling pathway. This antibody pair provides a sensitive and valuable tool to study the factors that induce DNA damage and/or affect DNA repair, and allows one to explore the linkage between DNA damage, cell cycle checkpoints, and initiation of apoptosis. Both antibodies provided in the kit are carefully titrated and optimized together to ensure maximal performance when run in multiplex, alleviating the need for any additional optimization. This kit contains optimized fixation, permeabilization, and assay buffers to provide researchers with a complete solution for DNA damage signaling analysis.

      The Muse™ Multi-Color DNA Damage Kit is optimized on the Muse™ Cell Analyzer. The Muse™ System makes sophisticated fluorescent-based analysis fast, easy, convenient, and affordable. Sample preparation is minimal, and after loading samples onto the Muse™ Cell Analyzer, the intuitive software provides detailed or summary analysis of your cell sample in a few short steps. Sufficient reagent is provided for the preparation and analysis of 50 tests.
      For Research Use Only; not for use in diagnostic procedures.
      References
      Product Information
      Instrument compatibility
      • Muse Cell Analyzer
      Applications
      ApplicationThis kit is designed to enable researchers a quick and easy way to detect the activation of ATM and H2A.X using the Muse® Cell Analyzer.
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size50 Tests
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Muse™Multi-Color DNA Damage Kit SDS

      Title

      Safety Data Sheet (SDS) 

      Brochure

      Title
      Muse Cell Analyzer Service Plans (MilliporeSigma)
      Experience simple, affordable flow cytometry.

      Technical Info

      Title
      Application Note: A quantitative approach for interrogation of H2A.X and ATM DNA damage signaling using rapid benchtop flow cytometry
      White Paper: Individual cell analysis for protein expression and cell health markers presents a rapid, quantitative alternative to bulk detection methods for fundamental assays.

      Posters

      Title
      Muse Cell Health Poster: A World of Cell Health Between Life and Death (MilliporeSigma)

      User Guides

      Title
      Muse™ Multi-Color DNA Damage Kit User’s Guide