MCH200104 Muse® MAPK Activation Dual Detection Kit

MCH200104
50 Tests  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMCH200104
      Trade Name
      • Muse
      DescriptionMuse® MAPK Activation Dual Detection Kit
      OverviewEMD Millipore’s Muse™ Activation Dual Detection kits are a series of products which include a pair of antibodies that bind to the same protein; one to detect total protein expression and another to detect the phosphorylated form of the same target. Two parameter analysis allows specific detection of phosphorylation and eliminates false positives while enhancing the signal to noise ratio. The Muse™ MAPK Activation Dual Detection Kit includes two directly conjugated antibodies, a phospho-specific anti-phospho-ERK1/2 (Thr202/Tyr204, Thr185/Tyr187)-Phycoerythrin and an
      anti-ERK1/2-PECy5 conjugated antibody to measure total levels of ERK. This two color kit is designed to measure the extent of MAPK phosphorylation relative to the total MAPK expression in any given cell population. By doing such, the levels of both the total and phosphorylated protein can be measured simultaneously in the same cell, resulting in a normalized and accurate measurement of MAPK activation after stimulation. Moreover, simultaneous measurement of both total and phospho-ERK1/2 confirms target specificity of the phosphorylation event. Together, a total and phospho antibody duo performed in multiplex provides an enhanced and more reliable detection of the phospho: total ratio within a mixed population.
      All Muse™ Dual Detection kits are optimized, and designed for use, on the Muse™ Cell Analyzer. The antibody pair provided in the kit has been carefully titrated to ensure the ability to measure total and phospho-ERK1/2 simultaneously on the same protein for accurate determination of protein level and activation. This kit contains optimized fixation, permeabilization, and assay buffers to provide researchers with a complete solution for MAPK signaling analysis. Data generated using the Muse™ Cell Analyzer with the Muse™ software provides:
      • Percentage of inactivated cells
      • Percentage of activated cells (via phosphorylation)
      • Percentage of non-expressing cells
      References
      Product Information
      Instrument compatibility
      • Muse Cell Analyzer
      Applications
      ApplicationThe Muse MAPK Activation Dual Detection Kit includes two directly conjugated antibodies, a phospho-specific anti-phospho-ERK1/2 (Thr202/Tyr204, Thr185/Tyr187)-Phycoerythrin & an anti-ERK1/2-PECy5 conjugated antibody to measure total levels of ERK.
      Biological Information
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      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
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      Material Size50 Tests
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      Documentation

      Protocols

      Title
      Muse™ MAPK Activation Dual Detection Kit

      SDS

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      Safety Data Sheet (SDS) 

      Brochure

      Title
      Muse Cell Analyzer Service Plans (MilliporeSigma)
      Experience simple, affordable flow cytometry.

      Technical Info

      Title
      White Paper: Individual cell analysis for protein expression and cell health markers presents a rapid, quantitative alternative to bulk detection methods for fundamental assays.

      Posters

      Title
      Muse Cell Health Poster: A World of Cell Health Between Life and Death (MilliporeSigma)

      User Guides

      Title
      Muse™ MAPK Activation Dual Detection Kit