Key Specifications Table
|Species Reactivity||Key Applications||Host||Format||Antibody Type|
|H||ELISA, RIA, WB||M||Purified||Monoclonal Antibody|
|Description||Mouse Anti-Human IgG4 Antibody, clone HP6025, Fc|
|Presentation||Purified immunoglobulin. Liquid in 0.2 M phosphate buffer, 0.25 M NaCl, pH 7.6, with 0.1% sodium azide.|
|Safety Information according to GHS|
|Storage and Shipping Information|
|Storage Conditions||Maintain at +4°C. DO NOT FREEZE.|
|Material Size||500 µg|
|Reference overview||Pub Med ID|
|Prospective randomized controlled study comparing cell block method and conventional smear method for pancreatic juice cytology. |
Yutaka Noda,Naotaka Fujita,Go Kobayashi,Kei Ito,Jun Horaguchi,Takashi Obana,Shinsuke Koshita,Yoshihide Kanno,Yasunobu Yamashita,Yuhei Kato,Takahisa Ogawa,Takashi Tsuchiya,Masaya Oikawa,Takashi Sawai,Hiroyuki Kanno,Akira Kurose
Digestive endoscopy : official journal of the Japan Gastroenterological Endoscopy Society 24 2012
? To elucidate the diagnostic efficacy of the cell block (CB) method by comparing it with that of conventional smear cytology for pancreatic juice obtained by endoscopic retrograde cholangiopancreatography (ERCP) in a randomized controlled trial fashion.
|Diagnostic efficacy of the cell block method in comparison with smear cytology of tissue samples obtained by endoscopic ultrasound-guided fine-needle aspiration. |
Yutaka Noda,Naotaka Fujita,Go Kobayashi,Kei Itoh,Jun Horaguchi,Osamu Takasawa,Takashi Obana,Shinsuke Koshita,Yoshihide Kanno,Takashi Suzuki,Dai Hirasawa,Toshiki Sugawara,Tetsuya Ohira,Yoshihiro Harada,Takashi Tsuchiya,Takashi Sawai,Miwa Uzuki,Akira Kurose
Journal of gastroenterology 45 2010
The diagnostic efficacy of endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA) cytology may vary greatly depending on the treatment of the samples obtained and the level of proficiency of the cytopathologist or cytoscreener.
|Monoclonal antibody-based solid-phase immunoenzymometric assays for quantifying human immunoglobulin G and its subclasses in serum. |
Papadea, C, et al.
Clin. Chem., 31: 1940-5 (1985) 1985
We developed quantitative immunoenzymometric assays for human IgG and its subclasses by using monoclonal antibodies, an avidin-biotin detection system and, as the calibrant, the U.S. National Reference Preparation for Specific Human Proteins. The assays are sensitive (detecting as little as 6 micrograms/L), precise (average inter-assay CV less than 11%), and vary linearly with concentrations over a five- to 10-fold range, depending on the monoclonal antibody. We evaluated 22 different monoclonal antibodies, many of which remained highly reactive when immobilized in wells of microtiter plates coated with bovine serum albumin-glutaraldehyde to "capture" total IgG or subclasses of IgG in the sample. We demonstrated the specificity of the most reactive antibodies by using a panel of 20 purified myeloma proteins. The sum of IgG subclass concentrations correlated well (r = 0.84, p less than 0.001) with the total IgG measured in sera from 63 apparently healthy adults (26 men, 37 women). We estimated 95 percentile reference intervals for the immunoglobulins in these subjects and determined the following mean percentage distributions of IgG subclasses: IgG1 49, IgG2 33, IgG3 9, and IgG4 7. The availability of these assays should facilitate studies of the clinical significance of the subclasses.
|Anti-IgG4, Fc fragment, clone HP6025 - Data Sheet|