411550 | Mouse Anti-Human IgG, Fc Fragment Specific (HP6043) Peroxidase Conjugate

411550
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      Overview

      Replacement Information

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      411550-500UG
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          Glass bottle 500 μg
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          Description
          Catalogue Number411550
          Brand Family Calbiochem®
          References
          ReferencesHamilton, R.G. 1993. Clin. Chem. 39, 1988.
          Hamilton, R.G. and Morrison, S.M. 1993. J. Immunolo. Meth. 158, 107.
          Hamilton, R.G. 1990. Ann. Biol. Clin. 48, 473.
          Hamilton, R.G., et al. 1988. J. Immunoassay. 9, 275.
          Hamilton, R.G., et al. 1987. Hybridoma 6, 204.
          Nakane, P.K. and Kawaoi A., 1974. J. Histochem Cytochem. 22, 1084.
          Product Information
          FormLiquid
          FormulationIn PBS, 10 mg/ml BSA.
          PreservativeNone
          Applications
          Application ReferencesEnzyme Immunoassay Hamilton, R.G., et al. 1987. Hybridoma 6, 204. Cross-Reactivity, Myeloma Proteins Hamilton, R.G., et al. 1988. J. Immunoassay. 9, 275. Hamilton, R.G., et al. 1987. Hybridoma 6, 204.
          Key Applications Enzyme Immunoassay
          Application NotesEnzyme Immunoassay (1:1000, see application references)
          Application CommentsAntibody should be titrated for optimal results in individual systems.

          Cross-reactivity by ELISA against human myeloma proteins:
          Human IgG1: 100%
          Human IgG2: 100%
          Human IgG3: 100%
          Human IgG4: 100%
          Human IgM: <0.2%
          Human IgA: <0.1%
          Human IgE: <0.1%

          Cross-reactivity by ELISA against chimeric antibodies:
          Human IgG1: 100%
          Human IgG2: 100%
          Human IgG3: 100%
          Human IgG4: 100%
          Human IgM: <0.01%
          Human IgA: <0.01%
          Human IgE: <0.01%

          There is no detectable binding to bovine, goat, horse sheep IgG, so these species sera can be used for assay blocking buffers. Modification of Nakane and Kawaoi method used for enzyme coupling.
          Biological Information
          CloneHP6043
          HostMouse
          IsotypeIgG
          Antibody TypeMonoclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C). Avoid more than 5 freeze/thaw cycles.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          411550

          References

          Reference overview
          Hamilton, R.G. 1993. Clin. Chem. 39, 1988.
          Hamilton, R.G. and Morrison, S.M. 1993. J. Immunolo. Meth. 158, 107.
          Hamilton, R.G. 1990. Ann. Biol. Clin. 48, 473.
          Hamilton, R.G., et al. 1988. J. Immunoassay. 9, 275.
          Hamilton, R.G., et al. 1987. Hybridoma 6, 204.
          Nakane, P.K. and Kawaoi A., 1974. J. Histochem Cytochem. 22, 1084.

          Brochure

          Title
          Antibody Sourcebook!" Second Edition
          Enzyme-Linked Detection Systems Technical Bulletin
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision11-March-2009 JSW
          ApplicationEnzyme Immunoassay (1:1000, see application references)
          DescriptionPurified mouse monoclonal antibody conjugated to horseradish peroxidase. Recognizes human IgG Fc fragment.
          BackgroundThe HP series of murine human Ig-specific monoclonal antibodies was produced from documented hybridoma cell lines that were developed at the Centers for Disease Control in Atlanta, Georgia. Laboratory analysis of each monoclonal antibody lot is performed in two stages during the production of ascites. First, the cell culture medium is analyzed for the presence and relative amount of human Ig-specific antibody by ELISA before injection of cells into mice. Second, collected ascites are analyzed for potency and antibody specificity using ELISA and isoelectric focusing (IEF), often in combination with an affinity immunoblot. For more information, the CALBIOCHEM® technical monograph, The Human IgG Subclasses, is available free of charge.
          HostMouse
          CloneHP6043
          IsotypeIgG
          FormLiquid
          FormulationIn PBS, 10 mg/ml BSA.
          Concentration Label Please refer to vial label for lot-specific concentration
          PreservativeNone
          CommentsAntibody should be titrated for optimal results in individual systems.

          Cross-reactivity by ELISA against human myeloma proteins:
          Human IgG1: 100%
          Human IgG2: 100%
          Human IgG3: 100%
          Human IgG4: 100%
          Human IgM: <0.2%
          Human IgA: <0.1%
          Human IgE: <0.1%

          Cross-reactivity by ELISA against chimeric antibodies:
          Human IgG1: 100%
          Human IgG2: 100%
          Human IgG3: 100%
          Human IgG4: 100%
          Human IgM: <0.01%
          Human IgA: <0.01%
          Human IgE: <0.01%

          There is no detectable binding to bovine, goat, horse sheep IgG, so these species sera can be used for assay blocking buffers. Modification of Nakane and Kawaoi method used for enzyme coupling.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C). Avoid more than 5 freeze/thaw cycles.
          Toxicity Standard Handling
          ReferencesHamilton, R.G. 1993. Clin. Chem. 39, 1988.
          Hamilton, R.G. and Morrison, S.M. 1993. J. Immunolo. Meth. 158, 107.
          Hamilton, R.G. 1990. Ann. Biol. Clin. 48, 473.
          Hamilton, R.G., et al. 1988. J. Immunoassay. 9, 275.
          Hamilton, R.G., et al. 1987. Hybridoma 6, 204.
          Nakane, P.K. and Kawaoi A., 1974. J. Histochem Cytochem. 22, 1084.
          Application referencesEnzyme Immunoassay Hamilton, R.G., et al. 1987. Hybridoma 6, 204. Cross-Reactivity, Myeloma Proteins Hamilton, R.G., et al. 1988. J. Immunoassay. 9, 275. Hamilton, R.G., et al. 1987. Hybridoma 6, 204.