A part of MilliporeSigma

Montage Antibody Purification Kits

Designed for fast and easy antibody purification from serum, ascites or cell culture supernatants.

Overview

Specifications

Ordering Information

Montage Antibody Purification Kits Clear Sorting & Filtering
Catalog Numbericon Descriptionicon Pack Sizeicon
LSK2ABA20Montage Antibody Purification Kit with PROSEP-A media 2 columns (20 purifications) Show Pricing & Availability
LSK2ABA60Montage Spin Columns with PROSEP-A media 6 columns (60 purifications) Show Pricing & Availability
LSK2ABG20Montage Antibody Purification Kit with PROSEP-G media 2 columns (20 purifications) Show Pricing & Availability
LSK2ABG60Montage Spin Columns with PROSEP-G media 6 columns (60 purifications) Show Pricing & Availability
SCGP00525Steriflip-GP, 0.22 µm, polyethersulfone, gamma irradiated 25 Show Pricing & Availability
UFC903008Amicon Ultra-15 Centrifugal Filter Unit with Ultracel-30 membrane 8 Show Pricing & Availability
UFC903024Amicon Ultra-15 Centrifugal Filter Unit with Ultracel-30 membrane 24 Show Pricing & Availability
UFC903096Amicon Ultra-15 Centrifugal Filter Unit with Ultracel-30 membrane 96 Show Pricing & Availability

Back To Top

    Documentation

    Data Sheet

    Title
    Montage Antibody Purification Kits

    Posters

    Title
    Poster: Rapid, Ultrafiltration-based Method for Purification of Monoclonal Antibodies from Hybridoma Supernatants

    References

    Reference overview
    Antibody Purification with Affinity Spin Columns
    Kavonian, Mark,BioScience Technology, July 2003
    BioScience Technology, July 2003  2003

    Considerations During Development of a Protein A-Based Antibody Purification Process
    Iyer et al.,Biopharm, January 2002, pp 14-20
    Biopharm, January 2002, pp 14-20  2002

    A sensitive enzyme linked immunoadsorbent assay (ELISA) for the detection of staphylococcal prtein A (SpA) present as a trace contaminant of murine immunoglobulins purified on immobilized protein A
    Miguel A.J. Godfrey, Piotr Kwasowski, Roland Clift and Vincent Marks
    Journal of Immunological Methods, 149(1992) 21-27, 1992  1992

    FAQ

    QuestionAnswer
    What is the shelf life of the Prosep A and Prosep G spin columns? The spin columns/media are under warranty for 2 years provided they are stored at 2-8 C.
    Do I need to filter the buffers provided with the Montage Antibody Purification kit? The buffers supplied with the kit are pre-filtered for immediate use.
    Do I need to pre-filter my sample before loading it onto a Prosep A or ProSep G spin column? All samples must be filtered through a 0.22 mm filter immediately before loading the sample onto the column. We recommend using a Steriflip-GP 0.22 mm filter.
    What is the binding capacity of the Prosep A spin column? Using the Prosep A spin columns we have been able to bind >20 mg of Rabbit IgG.
    What is the maximum volume I can load onto the Prosep A and Prosep G spin columns? You can load up to 20 ml in a Prosep A or Prosep G sping column for a swinging bucket rotor and up to 10 ml for a fixed angle rotor. We recommend that you spin it in a swinging bucket rotor.
    Why do you suggest that the Prosep A spin columns be spun at 100-150 xg for 20 minutes? The low speed, twenty minute centrifugation maximizes IgG / resin contact which increases IgG binding efficiency.
    What is the minimum elution volume for the Prosep A and Prosep G spin columns? We suggest 10 ml. Smaller volumes can be used but they may result in incomplete IgG recovery. If you use a smaller volume the neutralization buffer volume should also be adjusted accordingly.
    How do I regenerate the Prosep A spin columns? We recommend that you wash the plugs with 10 ml of Elution Buffer B2 by centrifuging at 500 xg got 5 minutes. re-equilibrate the media with 5 ml of Binding Buffer A by centrifuging the spin column at 500 xg for 2 minutes. Proceed to step 1 of the antibody purification protocol for immediate re-use or store the plugs without their end caps in 5 ml of Binding Buffer a in a 15 ml screw-capped tube for future use.
    Can I autoclave the Prosep A and Prosep G spin columns? The Prosep A and Prosep G spin columns cannot be autoclaved.
    Is pH an important parameter to control during Protein A spin chromotgraphy? The elution pH is the most critical variable. For Protein A, elution by pH steps (starting at pH 6.0) may fractionate different species (weaker binding bovine IgG from target antibodies) or subclasses. High pH (pH 8.0-9.0) in conjunction with high salt may promote binding of mouse IgG to Protein A. The binding buffer pH should normally be higher that pH 6.0-7.0.

    User Guides

    Title
    Montage Antibody Purification Kit and Spin Columns with PROSEP-A Media
    Montage Antibody Purification and Spin Columns with PROSEP-G Media

    Related Products & Applications

    Product Families

    Related Products By: Application Facete

    Related Products By: Brand Facete

    Categories

    Life Science Research > Protein Detection and Quantification > Immunoassays > Immunoprecipitation (IP) > Agarose Beads for IP & Antibody Purification
    Life Science Research > Antibodies and Assays > Immunoassays > Immunoprecipitation (IP) > Agarose Beads for IP & Antibody Purification
    Life Science Research > Protein Sample Preparation > Protein Purification > Agarose Bead Affinity Purification > Agarose Beads for IP & Antibody Purification