530390 Mitosis Inhibitor, AK301 - Calbiochem

530390
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      Overview

      Replacement Information

      Key Specifications Table

      Empirical Formula
      C₁₉H₂₁ClN₂O₂

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      5.30390.0001
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          Glass bottle 10 mg
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          Description
          OverviewA cell permeable piperazine derivative that induces irreversible mitotic arrest in colon cancer cells (IC50 = 115 nM in HT29 cells) and increases their sensitivity to TNF-induced apoptosis (EC50 = 172 nM). Also shown to enhance FasL and TRAIL-induced caspase-3 activity in colon cancer cells. Binds to the chochicine-binding domain on b-tubulin with high affinity, although in a different orientation than cholchicine, and reduces tubulin polymerization with formation of multiple microtubule organizing centers with Aurora kinase A and g-tubulin. Induces G2/M arrest in both HT29 colon cancer cells and WI38 lung fibroblasts, however, this effect is more pronounced in cancer cells.
          Catalogue Number530390
          Brand Family Calbiochem®
          SynonymsTNF sensitizing agent
          References
          ReferencesChopra, A. S., et al. 2013. J. Bio. Chem. 289, 2978.
          Product Information
          FormWhite solid
          Hill FormulaC₁₉H₂₁ClN₂O₂
          Chemical formulaC₁₉H₂₁ClN₂O₂
          ReversibleY
          Applications
          Biological Information
          Primary Targettubulin
          Primary Target IC<sub>50</sub>115 nM
          Purity≥98% by HPLC
          Physicochemical Information
          Cell permeableY
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Protect from Light Protect from light
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
          Packaging Information
          Packaged under inert gas Packaged under inert gas
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          References

          Reference overview
          Chopra, A. S., et al. 2013. J. Bio. Chem. 289, 2978.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision09-May-2014 JSW
          SynonymsTNF sensitizing agent
          DescriptionA cell permeable piperazine derivative that induces irreversible mitotic arrest in colon cancer cells (IC50 = 115 nM in HT29 cells) and increases their sensitivity to TNF-induced apoptosis (EC50 = 172 nM). Also shown to enhance FasL and TRAIL-induced caspase-3 activity in colon cancer cells. Binds to the chochicine-binding domain on b-tubulin with high affinity, although in a different orientation than cholchicine, and reduces tubulin polymerization with formation of multiple microtubule organizing centers with Aurora kinase A and g-tubulin. Induces G2/M arrest in both HT29 colon cancer cells and WI38 lung fibroblasts, however, this effect is more pronounced in cancer cells.
          FormWhite solid
          Intert gas (Yes/No) Packaged under inert gas
          Chemical formulaC₁₉H₂₁ClN₂O₂
          Purity≥98% by HPLC
          SolubilityDMSO (25 mg/ml)
          Storage +2°C to +8°C
          Protect from light
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
          Toxicity Standard Handling
          ReferencesChopra, A. S., et al. 2013. J. Bio. Chem. 289, 2978.