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FCMAB151F | Milli-Mark Anti-Y14 -FITC Antibody, clone 4C4

FCMAB151F
100 tests  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H FC M FITC Monoclonal Antibody
      Description
      Catalogue NumberFCMAB151F
      Trade Name
      • Milli-Mark
      DescriptionMilli-Mark Anti-Y14 -FITC Antibody, clone 4C4
      Alternate Names
      • Binder of OVCA1-1
        RNA binding motif protein 8A
        RNA binding motif protein 8B
        RNA-binding motif protein 8A
        RNA-binding protein Y14
        Ribonucleoprotein RBM8A
        ribonucleoprotein RBM8
      Background InformationY14 (RNA binding motif protein 8A or RBM8A), an RNA-binding protein, contains a conserved RNA-binding motif. Y14 is found predominantly in the nucleus, although it is also present in the cytoplasm. It is preferentially associated with mRNAs produced by splicing, including both nuclear mRNAs and newly exported cytoplasmic mRNAs. It is thought that the protein remains associated with spliced mRNAs as a tag to indicate where introns had been present, thus coupling pre- and post-mRNA splicing events. Previously, it was thought that two genes encode this protein, RBM8A and RBM8B; it is now thought that the RBM8B locus is a pseudogene. Two alternative start codons result in two forms of the protein, and this gene also uses multiple polyadenylation sites.
      References
      Product Information
      FormatFITC
      Control
      • HeLa Cells
      PresentationPurified mouse monoclonal IgG2bκ conjugated to FITC in PBS with 0.1% sodium azide and 15 mg/mL BSA.
      Applications
      ApplicationThis Milli-Mark Anti-Y14 -FITC Antibody, clone 4C4 is validated for use in FC for the detection of Y14.
      Key Applications
      • Flow Cytometry
      Biological Information
      ImmunogenPurified human Y14.
      EpitopeUnknown
      Clone4C4
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes Y14.
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      Species Reactivity NoteTested and passed on Human. Unconjugated antibody Cat No. 05-1511 known to react with mouse and rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a protein with a conserved RNA-binding motif. The protein is found predominantly in the nucleus, although it is also present in the cytoplasm. It is preferentially associated with mRNAs produced by splicing, including both nuclear mRNAs and newly exported cytoplasmic mRNAs. It is thought that the protein remains associated with spliced mRNAs as a tag to indicate where introns had been present, thus coupling pre- and post-mRNA splicing events. Previously, it was thought that two genes encode this protein, RBM8A and RBM8B; it is now thought that the RBM8B locus is a pseudogene. Two alternative start codons result in two forms of the protein, and this gene also uses multiple polyadenylation sites. [provided by RefSeq]
      Gene Symbol
      • RBM8A
      • BOV-1
      • BOV-1A
      • BOV-1B
      • BOV-1C
      • MDS014
      • RBM8A
      • RBM8B
      • Y14
      • ZNRP
      • ZRNP1
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Core components of the EJC, that remains bound to spliced mRNAs throughout all stages of mRNA metabolism, functions to mark the position of the exon-exon junction in the mature mRNA and thereby influences downstream processes of gene expression including mRNA splicing, nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). The heterodimer MAGOH-RBM8A interacts with PYM that function to enhance the translation of EJC-bearing spliced mRNAs by recruiting them to the ribosomal 48S preinitiation complex. Remains associated with mRNAs in the cytoplasm until the mRNAs engage the translation machinery. Its removal from cytoplasmic mRNAs requires translation initiation from EJC-bearing spliced mRNAs. Associates preferentially with mRNAs produced by splicing. Does not interact with pre-mRNAs, introns, or mRNAs produced from intronless cDNAs. Associates with both nuclear mRNAs and newly exported cytoplasmic mRNAs. Complex with MAGOH is a component of the nonsense mediated decay (NMD) pathway.


      SUBUNIT STRUCTURE: Heterodimer with MAGOH. Part of the exon junction complex (EJC) core complex that contains CASC3, EIF4A3, MAGOH and RBM8A. Found in a mRNA splicing-dependent EJC, at least composed of ACIN1, CASC3, DEK, EIF4A3, MAGOH, NCBP1, NCBP2, PNN, NXF1, RBM8A, RENT2, RENT3A, RENT3B, RNPS1, SAP18, SRRM1 and THOC4. Found in a post-splicing complex with NXF1, RBM8A, RENT1, RENT2, RENT3A, RENT3B and RNPS1. Interacts with BAT1, MAGOH, OVCA1, RENT3B, RNPS1, SRRM1 and THOC4. Identified in the spliceosome C complex, at least composed of AQR, ASCC3L1, C19orf29, CDC40, CDC5L, CRNKL1, DDX23, DDX41, DDX48, DDX5, DGCR14, DHX35, DHX38, DHX8, EFTUD2, FRG1, GPATC1, HNRPA1, HNRPA2B1, HNRPA3, HNRPC, HNRPF, HNRPH1, HNRPK, HNRPM, HNRPR, HNRPU, KIAA1160, KIAA1604, LSM2, LSM3, MAGOH, MORG1, PABPC1, PLRG1, PNN, PPIE, PPIL1, PPIL3, PPWD1, PRPF19, PRPF4B, PRPF6, PRPF8, RALY, RBM22, RBM8A, RBMX, SART1, SF3A1, SF3A2, SF3A3, SF3B1, SF3B2, SF3B3, SFRS1, SKIV2L2, SNRPA1, SNRPB, SNRPB2, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, SNW1, SRRM1, SRRM2, SYF2, SYNCRIP, TFIP11, THOC4, U2AF1, WDR57, XAB2 and ZCCHC8. Interacts with WIBG/PYM; the interaction is direct and leads to dissociate the EJC from spliced mRNAs. Associates with polysomes.


      SUBCELLULAR LOCATION: Nucleus. Nucleus speckle. Cytoplasm. Note: Nucleocytoplasmic shuttling protein. Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA. Colocalizes with the core EJC, THOC4, NXF1 and UAP56 in the nucleus and nuclear speckles.


      TISSUE SPECIFICTY: Ubiquitous.


      SEQUENCE SIMILARLITIES: Belongs to the RBM8A family.


      Contains 1 RRM (RNA recognition motif) domain
      Molecular Weight~19.9kDa Calculated
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by flow cytometry using HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.
      Packaging Information
      Material Size100 tests
      Transport Information
      Supplemental Information
      Specifications

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      Catalog Number Description  
      04-1464 Anti-Y14 Antibody, clone 1F12 Show Pricing & Availability
      05-1511 Anti-Y14 Antibody, clone 4C4 Show Pricing & Availability

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies