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FCMAB143F | Milli-Mark Anti-RPA2 p34 -FITC Antibody, clone RPA20 1-46

100 tests  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H FC M FITC Monoclonal Antibody
      Catalogue NumberFCMAB143F
      Trade Name
      • Milli-Mark
      DescriptionMilli-Mark Anti-RPA2 p34 -FITC Antibody, clone RPA20 1-46
      Alternate Names
      • RF-A protein 2
        RP-A p32
        RP-A p34
        Replication factor A protein 2
        replication protein A2 (32kD)
        replication protein A2, 32kDa
        Replication protein A 32kDa subunit
        RF-A, replication factor-A protein 2
      Background InformationRPA is a heterotrimeric protein complex that binds specifically to single-stranded DNA (ssDNA). It is composed of three subunits, RPA1 (70 kDa), RPA2 (32 kDa), and RPA3 (14 kDa), and plays multiple roles in DNA metabolism. RPA is required for DNA replication initiation, as well as replication elongation. At the onset of DNA replication, RPA is loaded onto chromatin after the binding of Cdc45 to origins. RPA is needed for subsequent loading of DNA polymerase and other replication proteins to initiate DNA replication. After DNA replication begins, RPA moves with replication forks, stabilizing ssDNA and assisting in DNA synthesis. In addition to its replication function, RPA is also known to play essential roles in damage repair and recombination.The 32 kDa subunit, is phosphorylated by the cdc2 family of kinases when cells enter S-phase and in response to DNA damage by ATM, ATR, and DNA-PK. Alternate names for RPA32 include replication protein A 32 kDa subunit, RP-A, RF-A, replication factor-A protein 2, p32, p34, RPA2, REPA2, and RPA32.
      Product Information
      • HeLa cells
      PresentationPurified mouse monoclonal IgG1κ conjugated to FITC in PBS with 0.1% sodium azide and 15 mg/mL BSA.
      ApplicationThis Milli-Mark Anti-RPA2 p34 -FITC Antibody, clone RPA20 1-46 is validated for use in FC for the detection of RPA2 p34.
      Key Applications
      • Flow Cytometry
      Biological Information
      ImmunogenHis-tagged recombinant protein corresponding to human RPA2
      CloneRPA20, 1-46
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityAntibody recognizes RPA2
      Species Reactivity
      • Human
      Species Reactivity NoteTested and passed on Human. Unconjugated antibody part number 04-1481 known to react with Human and Mouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • RPA2
      • REPA2
      • RPA32
      • RPA34
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.

      SUBUNIT STRUCTURE: Heterotrimer of 70, 32 and 14 kDa chains. The DNA-binding activity may reside exclusively on the 70 kDa subunit. Binds to SERTAD3/RBT1. Interacts with TIPIN.

      SUBCELLULAR LOCATION: Nucleus. Note: Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage.

      PTM: Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDC2.
      Molecular Weight 32 kDa Observed
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by flow cytometry using HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.
      Packaging Information
      Material Size100 tests
      Transport Information
      Supplemental Information

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      Life Science Research > Antibodies and Assays > Primary Antibodies