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FCMAB145F | Milli-Mark Anti-CAF1 p150 -FITC Antibody, clone SS1, 1-3

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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H FC M FITC Monoclonal Antibody
      Catalogue NumberFCMAB145F
      Trade Name
      • Milli-Mark
      DescriptionMilli-Mark Anti-CAF1 p150 -FITC Antibody, clone SS1, 1-3
      Alternate Names
      • CAF-1 subunit A
        CAF-I 150 kDa subunit
        Chromatin assembly factor I p150 subunit
        chromatin assembly factor 1, subunit A (p150)
        chromatin assembly factor I (150 kDa)
      Background InformationChromatin assembly factor (CAF) is a heterotrimeric complex that belongs to the histone chaperone family. It is found in yeast, animals and plants, and facilitates the assembly of chromatin during DNA replication and repair. There are three CAF subunits: p48, p60, and p150. The p150 subunit interacts with heterochromatin protein 1, methyl-binding protein 1 and the Bloom’s syndrome DNA-helicase BLM. Mutations in CAF p150 have resulted in defects in embryo development.
      Product Information
      • HeLa cells
      PresentationPurified mouse monoclonal IgG1κ conjugated to FITC in PBS with 0.1% sodium azide and 15 mg/mL BSA.
      ApplicationMilli-Mark Anti-CAF1 p150 -FITC Antibody, clone SS1, 1-3 detects level of CAF1 p150 & has been published & validated for use in FC.
      Key Applications
      • Flow Cytometry
      Biological Information
      ImmunogenHis-tagged recombinant human protein corresponding to CAF-1 p150.
      CloneSS1, 1-3
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityAntibody recognizes CAF-1 p150
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryChromatin assembly factor I (CAF1) is a nuclear complex consisting of p50, p60 (CHAF1B; MIM 601245), and p150 (CHAF1A) subunits that assembles histone octamers onto replicating DNA in vitro (Kaufman et al., 1995 [PubMed 7600578])
      Gene Symbol
      • CHAF1A
      • CAF
      • CAF-1
      • CAF-Ip150
      • CAF1
      • CAF1B
      • CAF1P150
      • P150
      • hp150
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Core component of the CAF-1 complex, a complex thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. CHAF1A binds to histones H3 and H4. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci By similarity. The CCR4-NOT complex functions as general transcription regulation complex. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.

      SUBUNIT STRUCTURE: Homodimer. Part of the CAF-1 complex that contains RBBP4, CHAF1B and CHAF1A. CHAF1A binds directly to CHAF1B. Only minor amounts of RBBP4 are complexed with CHAF1A and CHAF1B in G1 phase. Part of the CCR4-NOT core complex that contains CHAF1A, CHAF1B, CNOT1, CNOT2, CNOT3, CNOT4, CNOT6 and CNOT8. CHAF1A binds directly to PCNA and to CBX1. Binds MBD1. Interacts directly with CBX5 via the PxVxL motif. During DNA replication, it forms a S phase-specific complex that facilitates DNA methylation and histone H3 'Lys-9' methylation during replication-coupled chromatin assembly and is at least composed of the CHAF1A, MBD1 and SETDB1. Component of the WINAC complex, at least composed of SMARCA2, SMARCA4, SMARCB1, SMARCC1, SMARCC2, SMARCD1, SMARCE1, ACTL6A, BAZ1B/WSTF, ARID1A, SUPT16H, CHAF1A and TOP2B.

      SUBCELLULAR LOCATION: Nucleus. Note: DNA replication foci.

      DEVELOPMENTAL STAGE: Active complex is found in G1, S and G2 phases.

      DOMAIN: Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain.

      PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.

      SEQUENCE SIMILARITIES: Belongs to the CHAF1A family.
      Molecular Weight 150 kDa Calculated
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by flow cytometry using HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.
      Packaging Information
      Material Size100 tests
      Transport Information
      Supplemental Information

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      Catalog Number Description  
      04-1522 Anti-CAF1 p150 Antibody, clone SS1, 1-3 Show Pricing & Availability

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      Life Science Research > Antibodies and Assays > Primary Antibodies