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FCMAB133F | Milli-Mark™ Anti-TRA-2-49 Antibody, clone TRA-2-49/6E, FITC Conjugate

100 tests  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H FC M FITC Monoclonal Antibody
      Catalogue NumberFCMAB133F
      Trade Name
      • Milli-Mark
      DescriptionMilli-Mark™ Anti-TRA-2-49 Antibody, clone TRA-2-49/6E, FITC Conjugate
      Background InformationIn humans, four isoforms of alkaline phosphatase exist: intestinal, placental, placenta-like (germ cell type) and tissue-nonspecific. The tissue-nonspecific isoform of alkaline phosphatase is present in bone, liver and kidney; it differs from the other forms by posttranslational modification.
      Undifferentiated human Embryonal Carcinoma (EC) and Embryonic Stem (ES) cells have been shown to express very high levels of Alkaline Phosphatase isozyme that is indistinguishable from the isozyme found in liver, bone and kidney. Expression levels of AP decrease following stem cell differentiation. This clone can be used to monitor the expression of the Human Liver / Bone / Kidney isozyme of Alkaline Phosphatase (AP), and hence the differentiation status of human EC and ES cells by flow cytometry.
      Product Information
      • 2102 Ep cells.
      PresentationPurified mouse monoclonal IgG1 conjugated to FITC in PBS with 0.1% sodium azide and 15 mg/mL BSA.
      ApplicationDetect TRA-2-49 using this Milli-Mark Anti-TRA-2-49 Antibody, clone TRA-2-49/6E, FITC Conjugate validated for use in FC.
      Key Applications
      • Flow Cytometry
      Working Solution Cells / test Total Reaction Volume
      5 μl 5 x 105 Cells / 95 μl PBS 100 μl
      10 μl 1 x 106 Cells / 90 μl PBS 100 μl
      Biological Information
      Immunogen2102Ep human Embryonal Carcinoma Cells
      SpecificityAntibody recognizes TRA-2-49.
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • TRA-2-49
      Purification MethodProtein A Purfied
      Molecular Weight 212/80/55 kDa Observed
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by flow cytometry using 2102 Ep cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8°C protected from light in undiluted aliquots for up to 6 months from date of receipt.
      Packaging Information
      Material Size100 tests
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Milli-Mark Anti-TRA-2-49-FITC, clone TRA-2-49/6E - 2128260 2128260
      Milli-Mark Anti-TRA-2-49-FITC, clone TRA-2-49/6E - 2043918 2043918
      Milli-Mark Anti-TRA-2-49-FITC, clone TRA-2-49/6E - 2109549 2109549
      Milli-Mark Anti-TRA-2-49-FITC, clone TRA-2-49/6E - 2172762 2172762
      Milli-Mark Anti-TRA-2-49-FITC, clone TRA-2-49/6E - NG11808962 NG11808962
      Milli-Mark Anti-TRA-2-49-FITC, clone TRA-2-49/6E - NRG1713071 NRG1713071
      Milli-Mark™ Anti-TRA-2-49-FITC, -2682521 2682521
      Milli-Mark™ Anti-TRA-2-49-FITC, clone TRA-2-49/6E 2485671
      Milli-Mark™ Anti-TRA-2-49-FITC, clone TRA-2-49/6E - 2526420 2526420
      Milli-Mark™ Anti-TRA-2-49-FITC, clone TRA-2-49/6E - 2560535 2560535
      Milli-Mark™ Anti-TRA-2-49-FITC, clone TRA-2-49/6E - 2561072 2561072
      Milli-Mark™ Anti-TRA-2-49-FITC, clone TRA-2-49/6E -2585797 2585797
      Milli-Mark™ Anti-TRA-2-49-FITC, clone TRA-2-49/6E -2632985 2632985
      Milli-Mark™ Anti-TRA-2-49-FITC, clone TRA-2-49/6E -2733677 2733677
      Milli-Mark™ Anti-TRA-2-49-FITC, clone TRA-2-49/6E - 2343600 2343600
      Milli-Mark™ Anti-TRA-2-49-FITC, clone TRA-2-49/6E - 2276379 2276379


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