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FCABS334APC | Milli-Mark™ Anti-REST-APC Antibody

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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R FC Rb APC Polyclonal Antibody
      Catalogue NumberFCABS334APC
      Trade Name
      • Milli-Mark
      DescriptionMilli-Mark™ Anti-REST-APC Antibody
      Alternate Names
      • Neural-restrictive silencer factor
      • RE1-silencing transcription factor
      • X2 box repressor
      • neuron restrictive silencer factor
      • repressor binding to the X2 box
      Background InformationNRSF (neuron-restrictive silencer factor, also designated XBR and REST for RE1-silencing factor) is a silencer protein that binds the DNA sequence element NRSE (neuron-restrictive silencer element). The binding of NRSF to the NRSE represses neuronal gene transcription in non-neuronal cells. Although NRSF is most highly expressed in non-neural tissues, it is also expressed in developing neurons and at low levels in the brain. NRSF contains nine zinc finger domains, but also exists as a C-terminally truncated form produced by alternative splicing. This variant, REST4, contains five of the zinc-finger domains and weakly binds DNA, yet is transported to the nucleus. NRSF associates with mSin3 and HDAC in ventricular myocytes, suggesting a role for NRSF outside the nervous system. Down-regulation of NRSF, which normally occurs upon neural differentiation, is necessary for the proper development of certain classes of neurons. NRSF is required to repress neuronal gene expression in vivo, in both extra-neural and undifferentiated neural tissue.
      Product Information
      • HeLa cells.
      PresentationPurified rabbit polyclonal IgG conjugated to APC in PBS with 0.1% sodium azide and 15 mg/mL BSA.
      ApplicationMilli-Mark Anti-REST-APC Antibody detects level of REST-APC & has been published & validated for use in FC.
      Key Applications
      • Flow Cytometry
      Biological Information
      ImmunogenLinear peptide corresponding to human REST.
      SpecificityAntibody recognizes REST.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteTested and passed on Human. Predicted on Mouse, Rat based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • REST
      • NRSF
      • XBR
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q13127 # Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier.

      SIZE: 1097 amino acids; 121888 Da

      SUBUNIT: Interacts with SIN3A, SIN3B and RCOR1.

      SUBCELLULAR LOCATION: Nucleus (Probable).

      TISSUE SPECIFICITY: Ubiquitous. Expressed at higher levels in the tissues of the lymphocytic compartment, including spleen, thymus, peripheral blood lymphocytes and ovary.

      SIMILARITY: SwissProt: Q13127 ## Contains 9 C2H2-type zinc fingers.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by flow cytometry using HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.
      Packaging Information
      Transport Information
      Supplemental Information

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      Life Science Research > Antibodies and Assays > Primary Antibodies