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FCMAB210F | Milli-Mark™ Anti-CD86-FITC Antibody, clone BU63

FCMAB210F
100 tests  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H FC M FITC Monoclonal Antibody
      Description
      Catalogue NumberFCMAB210F
      Trade Name
      • Milli-Mark
      DescriptionMilli-Mark™ Anti-CD86-FITC Antibody, clone BU63
      Alternate Names
      • T-lymphocyte activation antigen CD86
      • Activation B7-2 antigen
      • CTLA-4 counter-receptor B7.2
      • B70
      • FUN-1
      • BU63
      Background InformationCD86 (B70/B7-2) is together with CD80 (B7/B7-1) natural ligands for CD28 and CD152 (CTLA-4). It is a 70 kDa transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. The CD86 cDNA encodes a 323 amino acid polypeptide composed of an N-terminal signal peptide, a 223 amino acid extracellular segment, a 23 amino acid transmembrane domain, and a 61 amino acid cytoplasmic domain (Azuma, 1993). The extracellular domain contains eight sites for N-linked glycosylation and the intracellular region has three potential sites for protein kinase C-dependent phosphorylation. The extracellular portion contains a V-set and a C2-set immunoglobulin-like domain (Azuma, 1993; Engel, 1994).
      CD86 is expressed on resting monocytes and dendritic cells and on activated T, NK, and B cells (Azuma, 1993; Engel, 1994; Caux, 1994; Engel, 1995). CD86 is rapidly induced on the surface of activated B cells. This suggests that it is the major CD28 ligand and may play an important role for costimulation of T cells in the primary immune response (Azuma, 1993; Thompson, 1995).
      References
      Product Information
      FormatFITC
      Control
      • Human Lymphocytes
      PresentationPurified Mouse monoclonal IgG1 conjugated to FITC in 0.05M Tris-HCl buffer with 1% BSA, Sodium Azide at 0.097% at pH 7.2.
      Applications
      ApplicationMilli-Mark Anti-CD86-FITC Antibody, clone BU63 is an antibody against CD86 for use in FC.
      Key Applications
      • Flow Cytometry
      Biological Information
      ImmunogenHuman peripheral blood lymphocytes
      CloneBU63
      HostMouse
      SpecificityAntibody recognizes Human CD86 antigen.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in two transcript variants encoding different isoforms. Additional transcript variants have been described, but their full-length sequences have not been determined.
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryReceptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.
      Molecular Weight 70 kDa (Azuma, 1993)
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by flow cytometry using human lymphocytes
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8°C protected from light in undiluted aliquots for up to 6 months from date of receipt.
      Packaging Information
      Material Size100 tests
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Milli-Mark Anti-CD86-FITC, clone BU63 - 1987085 1987085
      Milli-Mark Anti-CD86-FITC, clone BU63 - 2188557 2188557
      Milli-Mark Anti-CD86-FITC, clone BU63 - 2204958 2204958
      Milli-Mark Anti-CD86-FITC, clone BU63 - NRG1775604 NRG1775604
      Milli-Mark™ Anti-CD86-FITC, -2750263 2750263
      Milli-Mark™ Anti-CD86-FITC, clone BU63 - 2514247 2514247
      Milli-Mark™ Anti-CD86-FITC, clone BU63 - 2446288 2446288
      Milli-Mark™ Anti-CD86-FITC, clone BU63 - 2446948 2446948
      Milli-Mark™ Anti-CD86-FITC, clone BU63 - 2450740 2450740
      Milli-Mark™ Anti-CD86-FITC, clone BU63 - 2287781 2287781
      Milli-Mark™ Anti-CD86-FITC, clone BU63 - 2293527 2293527

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies