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FCMAB215P | Milli-Mark™ Anti-CD138-PE Antibody, clone MI15

100 tests  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H FC M Phycoerythrin Monoclonal Antibody
      Catalogue NumberFCMAB215P
      Trade Name
      • Milli-Mark
      DescriptionMilli-Mark™ Anti-CD138-PE Antibody, clone MI15
      Alternate Names
      • Syndecan-1
      • SYND1
      Background InformationCD138 is a transmembrane proteoglycan, bearing heparin sulphates and sometimes chondriotin sulfates. It consists of a 30.5 kDa core protein with 5 putative glycosaminoglycans attachment sites. CD138 is a member of the syndecan family. The reported Mr of CD138 ranges from 85,000 to 92,000, presumably depending on the glycosaminoglycan pattern of the molecule (Wijdenes, 1997). Within the hematopoietic system, CD138 is mainly confined to late stages of B-cell differentiation. It is typically expressed at high cellular density in plasma cells (Gattei, 1999). In mature tissues, CD138 is expressed by simple and stratified epithelia, fibroblasts, stratified keratinocytes and endothelial cells (Wijdenes, 1997). Anti-CD138, MI15, was included in the Sixth International Workshop and Conference on Human Leucocytes Differentiation Antigens, and studies by a number of different laboratories confirmed its reactivity with CD138 (Wijdenes, 1997).
      Product Information
      • U266 cells
      PresentationPurified Mouse monoclonal IgG1 conjugated to PE in 0.05M Tris-HCl buffer with 1% BSA, Sodium Azide at 0.097% at pH 7.2.
      ApplicationDetect CD138 using this Milli-Mark Anti-CD138-PE Antibody, clone MI15 validated for use in FC.
      Key Applications
      • Flow Cytometry
      Biological Information
      ImmunogenA mixture of U266 and XG-1 human myeloma cell lines
      SpecificityAntibody recognizes Human CD138 antigen
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. Altered syndecan-1 expression has been detected in several different tumor types. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same protein.
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryCell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix.
      Molecular Weight85-92 kDa (Wijdenes, 1997)
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by flow cytometry using U266 cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8°C protected from light in undiluted aliquots for up to 6 months from date of receipt.
      Packaging Information
      Material Size100 tests
      Transport Information
      Supplemental Information

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      Life Science Research > Antibodies and Assays > Primary Antibodies