115943 Methylene blue (C.I.52015)

115943
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      Overview

      Replacement Information

      Key Specifications Table

      EC NumberHill FormulaChemical FormulaMolar Mass
      200-515-2 C₁₆H₁₈ClN₃S * x H₂O C₁₆H₁₈ClN₃S * x H₂O (x=2-3) 319.86 g/mol (anhydrous)

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      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      1159430025
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          Glass bottle 25 g
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          1159430100
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              Glass bottle 100 g
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              1159431000
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                  Metal can 1 kg
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                  Description
                  Catalogue Number115943
                  SynonymsMethylthioninium chloride, 3,7-Bis(dimethylamino)phenothiazinium chloride, Tetramethylthionine chloride
                  DescriptionMETHYLENE BLUE (C.I. 52015) FOR MICROSCO
                  OverviewMethylene blue (C.I. 52015) is a Certistain® dye that is used in bacteriology for an overview stain and as counterstaining for Ziehl-Neelsen method in sample material of human origin. Bacteria appear blue. The dye has a further important application in hematology: Methylene blue and eosin are the main components of many staining solutions and dye mixtures. Certistain® is the product group for seleceted high quality dyes that fulfill all quality requirements of IVD and CE registration.
                  References
                  Product Information
                  EC number200-515-2
                  Hill FormulaC₁₆H₁₈ClN₃S * x H₂O
                  Chemical formulaC₁₆H₁₈ClN₃S * x H₂O (x=2-3)
                  Molar Mass319.86 g/mol (anhydrous)
                  HS Code3204 13 00
                  Structure formula ImageStructure formula Image
                  Applications
                  ApplicationCertistain® dry dye used as an overview stain and as a counterstain
                  Biological Information
                  Physicochemical Information
                  Melting Point180 °C (decomposition)
                  pH value3 (10 g/l, H₂O, 20 °C)
                  Bulk density400 - 600 kg/m3
                  Solubility50 g/l
                  Dimensions
                  Materials Information
                  Toxicological Information
                  LD 50 oralLD50 Rat 1180 mg/kg
                  Safety Information according to GHS
                  Hazard Pictogram(s) Hazard Pictogram(s)
                  Hazard Statement(s)H302: Harmful if swallowed.
                  Signal WordWarning
                  Storage class10 - 13 Other liquids and solids
                  WGKWGK 2 obviously hazardous to water
                  Disposal3
                  Relatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C.
                  Safety Information
                  Hazard Symbols Hazard SymbolsHarmful
                  Categories of dangerharmful
                  R PhraseR 22
                  Harmful if swallowed.
                  Product Usage Statements
                  Storage and Shipping Information
                  StorageStore at +5°C to +30°C.
                  Packaging Information
                  Transport Information
                  Supplemental Information
                  Specifications
                  Dye content (spectrophotometrically)≥ 82 %
                  Identity
                  - Chloride

                  passes test
                  - UV/VIS-Spectrumpasses test
                  Absorption maximum λ max (ethanol 50 %)660 - 665 nm
                  Spec. Absorptivity A 1%/1cm (λmax; 0.003 g/l; ethanol 50 %)2250 - 2750
                  TLC-Testpasses test
                  Loss on drying (110 °C)10 - 15 %
                  Suitability for microscopypasses test

                  Documentation

                  Methylene blue (C.I.52015) SDS

                  Title

                  Safety Data Sheet (SDS) 

                  Methylene blue (C.I.52015) Certificates of Analysis

                  Product Number PackagingSpecificationLot Number
                  1159430025 Glass bottlePDF Specification Document
                  1159430100 Glass bottlePDF Specification Document
                  1159431000 Metal canPDF Specification Document

                  Brochure

                  Title
                  HARLECO Dyes and Stains

                  Instructions for Use

                  Title
                  115943 Methylene blue (C.I. 52015) for microscopy Certistain®

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                  Accessories (Secondary)

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                  Categories

                  Reagents, Chemicals and Labware > Inorganic Reagents > Safety Products and General Applications > Indicators > Redox Indicators
                  IVD/OEM Materials & Reagents > Microscopy > Dyes for Microscopy > Certistain® Dyes