17-10499 Magna MeRIP™ m6A Kit- Transcriptome-wide Profiling of N6-Methyladenosine

17-10499
10 Assays  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

       

      Contact Customer Service

      Overview

      Replacement Information

      Key Specifications Table

      Key Applications
      RNA Detection, Purification with magnetic beads, RIP
      Description
      Catalogue Number17-10499
      Trade Name
      • EZ-Magna RIP
      DescriptionMagna MeRIP™ m6A Kit- Transcriptome-wide Profiling of N6-Methyladenosine
      OverviewN6-methyladenosine (m6A) represents an abundant RNA modification that is conserved across many different species, ranging from plants, to yeast, to mammals. Similar to the 5-methylcytosine (5-mc) modification of DNA, m6A is a reversible chemical modification of RNA. m6A RNA modifications are an emerging topic in controlling cell fate transitions in mammalian embryonic stem cells. m6A is one of the most prevalent modifications on both mRNAs and noncoding RNAs in eukaryotes with an estimated 12,000 m6A sites in over 7,000 genes. The mark is deposited by a heterodimer of methyltransferase-like 3 and 14 (Mettl3 and Mettl14) and can be removed by the RNA demethylase enzymes FTO and ALKBH5. Obesity risk gene FTO encodes the first identified m6A demethylase. Mutations in FTO have been associated with increased risk for obesity and type II diabetes. Recently, impairment of the status of m6A regulation has lead to prolonged expression of Nanog in ES cells and the inability to exit self-renewal stages toward differentiation.

      Methylated (m6A) RNA immunoprecipitation (MeRIP) is a method to monitor the status of m6A and map the location of m6A RNA modifications transcriptome-wide. The Magna MeRIP M6A Kit uses the MeRIP method to enable identification and transcriptome-wide profiling of m6A RNA modifications. In the MeRIP assay, RNA is chemically fragmented into 100 nucleotides or smaller fragments followed by magnetic immunoprecipitation with a monoclonal antibody toward m6A. After immunoprecipitation, isolated RNA fragments can be subjected with qRT-PCR or RNA sequencing (MeRIP-seq). The streamlined protocol is easy to process and can produce results with high SN ratios.

      Features and Benefits

      • Complete collection of MeRIP valiidated reagents to streamline the MeRIP method.
      • High signal to noise ratios using high affinity m6A monoclonal antibody and A/G magnetic beads.
      • Stem cell validated.
      Alternate Names
      • RNA Methylation Kit
      • MeRIP-Seq Kit
      • N6-Methyladenosine Kit
      • M6A Immunoprecipitation Kit
      References
      Product Information
      Components
      • RNA Fragmentation Buffer 10X (0.5 mL)
      • 0.5M EDTA (0.5 mL)
      • IP Buffer 5X (15 mL)
      • Magnetic Beads A/G Blend (315 µL)
      • Anti-N6-methyladenosine (m6A), clone 17-3-4-1 (100 µg)
      • RNase Inhibitor (90 µL)
      • N6-Methyladenosine, 5′-monophosphate sodium salt (m6A) (10 mg)
      • Normal Mouse IgG (125 µg)
      • MeRIP Primers Human EEF1A1 Positive (75 µL)
      • MeRIP Primers Human EEF1A1 Negative (75 µL)
      Applications
      ApplicationThe Magna MeRIP™ m6A Kit streamlines the MeRIP (Methylated RNA Immunoprecipitation) method to enable identification and transcriptome-wide profiling of m6A (N6-methyladenosine) RNA methylation sites. Suitable for MeRIP-Seq and qRT-PCR of methylated RNA transcripts.
      Key Applications
      • RNA Detection
      • Purification with magnetic beads
      • RNA Binding Protein Immunoprecipitation (RIP)
      Application NotesThe Magna MeRIP M6A Kit provides sufficient reagents for 10 MeRIP reactions using 5 µg of mRNA or 5 MeRIP reactions when 300 µg of total RNA is used.

      Please read through the entire protocol and carefully plan your work before starting. The MeRIP method requires multiple steps and can be done over a two-day period or over multiple days. There are several stopping points to allow the method to be carried out over multiple days. The approximate time required for each step and potential stopping points are provided in the tables below.
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsUpon receipt, store components at the temperatures indicated on the labels.
      Kit components are stable for 6 months from date of shipment when stored as directed.
      Packaging Information
      Material Size10 Assays
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      References

      Reference overviewPub Med ID
      R-2HG Exhibits Anti-tumor Activity by Targeting FTO/m6A/MYC/CEBPA Signaling.
      Su, R; Dong, L; Li, C; Nachtergaele, S; Wunderlich, M; Qing, Y; Deng, X; Wang, Y; Weng, X; Hu, C; Yu, M; Skibbe, J; Dai, Q; Zou, D; Wu, T; Yu, K; Weng, H; Huang, H; Ferchen, K; Qin, X; Zhang, B; Qi, J; Sasaki, AT; Plas, DR; Bradner, JE; Wei, M; Marcucci, G; Jiang, X; Mulloy, JC; Jin, J; He, C; Chen, J
      Cell  172  90-105.e23  2018

      Show Abstract
      29249359 29249359
      METTL14 Inhibits Hematopoietic Stem/Progenitor Differentiation and Promotes Leukemogenesis via mRNA m6A Modification.
      Weng, H; Huang, H; Wu, H; Qin, X; Zhao, BS; Dong, L; Shi, H; Skibbe, J; Shen, C; Hu, C; Sheng, Y; Wang, Y; Wunderlich, M; Zhang, B; Dore, LC; Su, R; Deng, X; Ferchen, K; Li, C; Sun, M; Lu, Z; Jiang, X; Marcucci, G; Mulloy, JC; Yang, J; Qian, Z; Wei, M; He, C; Chen, J
      Cell Stem Cell  22  191-205.e9  2018

      Show Abstract
      29290617 29290617
      RNA N6-methyladenosine methyltransferase-like 3 promotes liver cancer progression through YTHDF2-dependent posttranscriptional silencing of SOCS2.
      Chen, M; Wei, L; Law, CT; Tsang, FH; Shen, J; Cheng, CL; Tsang, LH; Ho, DW; Chiu, DK; Lee, JM; Wong, CC; Ng, IO; Wong, CM
      Hepatology  67  2254-2270  2018

      Show Abstract
      29171881 29171881
      Recognition of RNA N6-methyladenosine by IGF2BP proteins enhances mRNA stability and translation.
      Huang, H; Weng, H; Sun, W; Qin, X; Shi, H; Wu, H; Zhao, BS; Mesquita, A; Liu, C; Yuan, CL; Hu, YC; Hüttelmaier, S; Skibbe, JR; Su, R; Deng, X; Dong, L; Sun, M; Li, C; Nachtergaele, S; Wang, Y; Hu, C; Ferchen, K; Greis, KD; Jiang, X; Wei, M; Qu, L; Guan, JL; He, C; Yang, J; Chen, J
      Nat Cell Biol  20  285-295  2018

      Show Abstract
      29476152 29476152
      Promoter-bound METTL3 maintains myeloid leukaemia by m6A-dependent translation control.
      Barbieri, I; Tzelepis, K; Pandolfini, L; Shi, J; Millán-Zambrano, G; Robson, SC; Aspris, D; Migliori, V; Bannister, AJ; Han, N; De Braekeleer, E; Ponstingl, H; Hendrick, A; Vakoc, CR; Vassiliou, GS; Kouzarides, T
      Nature  552  126-131  2017

      Show Abstract
      29186125 29186125
      FTO Plays an Oncogenic Role in Acute Myeloid Leukemia as a N6-Methyladenosine RNA Demethylase.
      Li, Z; Weng, H; Su, R; Weng, X; Zuo, Z; Li, C; Huang, H; Nachtergaele, S; Dong, L; Hu, C; Qin, X; Tang, L; Wang, Y; Hong, GM; Huang, H; Wang, X; Chen, P; Gurbuxani, S; Arnovitz, S; Li, Y; Li, S; Strong, J; Neilly, MB; Larson, RA; Jiang, X; Zhang, P; Jin, J; He, C; Chen, J
      Cancer Cell  127-141  2017

      Show Abstract
      28017614 28017614

      Posters

      Title
      m6A RNA Modifications of Reprogramming Genes in Mouse and Human Embryonic Stem Cells

      Instructions for Use

      Title
      Magna MeRIP¿ m6A Kit