234376 Macrophage Colony-Stimulating Factor, Human, Recombinant, E. coli

234376
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      Overview

      Replacement Information

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      234376-5UG
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          Plastic ampoule 5 μg
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          Description
          OverviewRecombinant, human macrophage colony-stimulating factor expressed in E. coli. Stimulates formation of macrophage colonies from bone marrow hematopoietic progenitor cells. Increases osteoclastic bone resorption in adults. Induces cell death in HIV-1 infected monocytes.
          Catalogue Number234376
          Brand Family Calbiochem®
          SynonymsrhM-CSF
          References
          ReferencesEdwards, M., et al. 1998. Bone 22, 325.
          Bergamini, A., et al. 1994. Immunol. Lett. 42, 35.
          Hattersley, G., et al. 1991. Biochem. Biophys. Res. Commun. 177, 526.
          Product Information
          CAS number81627-83-0
          FormLyophilized
          FormulationLyophilized from sterile-filtered PBS, 50 µg of BSA/µg M-CSF.
          Applications
          Biological Information
          Biological activityED₅₀ = 0.5-1.5 ng/ml as measured in a cell proliferation assay using the M-CSF-dependent mouse monocytic cell line, M-NFS-60
          Purity≥97% by SDS-PAGE
          Physicochemical Information
          ContaminantsEndotoxin: <1.0 EU/µg M-CSF
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          RTECSGI5500000
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage ≤ -70°C
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for 1 month at 4°C or for 3 months at -70°C.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          234376

          References

          Reference overview
          Edwards, M., et al. 1998. Bone 22, 325.
          Bergamini, A., et al. 1994. Immunol. Lett. 42, 35.
          Hattersley, G., et al. 1991. Biochem. Biophys. Res. Commun. 177, 526.

          Brochure

          Title
          Growth Factors and Cytokines Technical Bulletin
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision09-June-2008 RFH
          SynonymsrhM-CSF
          DescriptionRecombinant, human macrophage colony-stimulating factor expressed in E. coli. Stimulates formation of macrophage colonies from bone marrow hematopoietic progenitor cells. Induces cell death in HIV-1 infected monocytes. Human M-CSF is active in murine systems and the recombinant cytokine is indistinguishable from the natural M-CSF in all biological assays tested.
          FormLyophilized
          FormulationLyophilized from sterile-filtered PBS, 50 µg of BSA/µg M-CSF.
          CAS number81627-83-0
          RTECSGI5500000
          Purity≥97% by SDS-PAGE
          ContaminantsEndotoxin: <1.0 EU/µg M-CSF
          Biological activityED₅₀ = 0.5-1.5 ng/ml as measured in a cell proliferation assay using the M-CSF-dependent mouse monocytic cell line, M-NFS-60
          SolubilityReconstitute to a concentration of ≥1 mg/ml with sterile PBS containing ≥0.1% HSA or BSA.
          Storage ≤ -70°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for 1 month at 4°C or for 3 months at -70°C.
          Toxicity Standard Handling
          ReferencesEdwards, M., et al. 1998. Bone 22, 325.
          Bergamini, A., et al. 1994. Immunol. Lett. 42, 35.
          Hattersley, G., et al. 1991. Biochem. Biophys. Res. Commun. 177, 526.

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          Categories

          Life Science Research > Proteins and Enzymes > Other Proteins