A part of MilliporeSigma

12-457 | MYPT1 (654-880)

100 µg  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
Request Pricing
Limited AvailabilityLimited Availability
Limited Quantities Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service


      Contact Customer Service

      Click To Print This Page


      Replacement Information
      Catalogue Number12-457
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionMYPT1 (654-880)
      OverviewROK substrate protein corresponding to amino acids 654-880 of human MYPT1 with an N-terminal GST tag. MW = 60 kDa.
      Product Information
      Presentation100mM Tris-HCl pH 8.0, 200mM NaCl, 10mM glutathione and 5μg/ml leupeptin. Frozen solution.
      Key Applications
      • Kinase Assay
      Biological Information
      Purity≥70% as judged by SDS PAGE and Coomassie staining.
      Sourceexpressed in E. coli
      Entrez Gene Number
      Entrez Gene SummaryMyosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase.
      Gene Symbol
      • PPP1R12A
      • MYPT1
      • MGC133042
      • MBS
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: O14974 # Regulates myosin phosphatase activity.
      SIZE: 1030 amino acids; 115281 Da
      SUBUNIT: PP1 comprises a catalytic subunit, PPP1CA, PPP1CB or PPP1CC, and one or several targeting or regulatory subunits. PPP1R12A mediates binding to myosin. Interacts with ARHA and CIT (By similarity). Binds PPP1R12B, ROCK1 and IL16.
      SUBCELLULAR LOCATION: Cytoplasm. Note=Along actomyosin filaments and stress fibers.
      PTM: Phosphorylated by CIT (Rho-associated kinase) (By similarity). Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR.
      SIMILARITY: SwissProt: O14974 ## Contains 6 ANK repeats.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated as a substrate in a Protein Kinase Assay.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery, centrifuge the vial prior to removing the cap.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      MYPT1 (654-880) (recombinant protein expressed in E. coli) - 2441918 2441918
      MYPT1 (654-880) (recombinant protein expressed in E. coli) - 2039789 2039789
      MYPT1 (654-880) (recombinant protein expressed in E. coli) - DAM1467553 DAM1467553
      MYPT1 (654-880) - 0610042655 0610042655
      MYPT1 (654-880) - 22328 22328
      MYPT1 (654-880) - 2360569 2360569
      MYPT1 (654-880) - 26439 26439
      MYPT1 (654-880) - 27451 27451
      MYPT1 (654-880) - 31414 31414
      MYPT1 (654-880) - DAM1487568 DAM1487568
      MYPT1 (654-880) - DAM1501805 DAM1501805
      MYPT1 (654-880) - DAM1524079 DAM1524079
      MYPT1 (654-880) - DAM1594211 DAM1594211
      MYPT1 (654-880) - DAM1655274 DAM1655274
      MYPT1 (654-880) - DAM1731512 DAM1731512
      MYPT1 (654-880) - DAM1745373 DAM1745373
      MYPT1 (654-880) -2566938 2566938
      MYPT1 (654-880) -2586456 2586456
      MYPT1 (654-880) -2669872 2669872
      MYPT1 (654-880) -2724298 2724298
      MYPT1 (654-880) -2736502 2736502
      MYPT1 (654-880) -2775504 2775504
      MYPT1 (654-880) -2802006 2802006


      Reference overviewPub Med ID
      Extracellular matrix controls insulin signaling in mammary epithelial cells through the RhoA/Rok pathway.
      Lee, Yi-Ju, et al.
      J. Cell. Physiol., 220: 476-84 (2009) 2009

      Show Abstract
      19391109 19391109
      Inhibitory phosphorylation site for Rho-associated kinase on smooth muscle myosin phosphatase.
      Feng, J, et al.
      J. Biol. Chem., 274: 37385-90 (1999) 1999

      Show Abstract
      10601309 10601309
      Activation of myosin phosphatase targeting subunit by mitosis-specific phosphorylation
      Totsukawa, G, et al
      J Cell Biol, 144:735-44 (1999) 1999

      10037794 10037794

      Related Products & Applications

      Product Families


      Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Kinase & Phosphatase Substrates
      Life Science Research > Proteins and Enzymes > Substrate & Control Proteins > Kinase & Phosphatase Substrates