444224 | MMP-2/MMP-9 Substrate II - Calbiochem

444224
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      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      444224-5MG
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          Plastic ampoule 5 mg
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          Description
          OverviewAn exceptional thiopeptide that is used as a substrate for the rapid, sensitive, and continuous spectrophotometric assay of Type IV collagenases, MMP-2 (gelatinase A), and MMP-9 (gelatinase B). Also acts as a substrate for vertebrate collagenase. Cleavage is monitored at 324 nm.
          Catalogue Number444224
          Brand Family Calbiochem®
          SynonymsAc-Pro-Leu-Gly-(2-mercapto-4-methylpentanoyl)-Leu-Gly-OEt, Gelatinase A/Gelatinase B Substrate II
          References
          ReferencesWeingarten, H., et al. 1985. Anal. Biochem. 147, 437.
          Weingarten, H., et al. 1985. Biochemistry 24, 6730.
          Product Information
          ATP CompetitiveN
          FormWhite solid
          Hill FormulaC₃₁H₅₃N₅O₈S
          Chemical formulaC₃₁H₅₃N₅O₈S
          ReversibleN
          Applications
          Biological Information
          Primary TargetSubstrate for the rapid, sensitive, and continuous spectrophotometric assay of Type IV collagenases, MMP-2 (gelatinase A), and MMP-9 (gelatinase B).
          Purity≥97% by HPLC
          Physicochemical Information
          Cell permeableN
          Peptide SequenceAc-Pro-Leu-Gly-SCH[CH₂CH(CH₃)₂]-CO-Leu-Gly-OC₂H₅
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage -20°C
          Protect from Light Protect from light
          Do not freeze Ok to freeze
          Special InstructionsUnstable in solution; reconstitute just prior to use.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          444224

          References

          Reference overview
          Weingarten, H., et al. 1985. Anal. Biochem. 147, 437.
          Weingarten, H., et al. 1985. Biochemistry 24, 6730.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision06-August-2008 RFH
          SynonymsAc-Pro-Leu-Gly-(2-mercapto-4-methylpentanoyl)-Leu-Gly-OEt, Gelatinase A/Gelatinase B Substrate II
          DescriptionAn exceptional thiopeptide that is used as a substrate for the rapid, sensitive, and continuous spectrophotometric assay for Type IV collagenases MMP-2 (gelatinase A) and MMP-9 (gelatinase B). Acts as a substrate for vertebrate collagenase. Cleavage is monitored at 324 nm.
          FormWhite solid
          Recommended reaction conditions50 mM HEPES buffer, pH 6.5-7.0, 10 mM CaCl2, 0.5-1 mM 4,4'-dithiodipyridine, 0.01-5 mM substrate, and desired amount of activated enzyme; incubate at 25-26° while continuously monitoring cleavage at 324 nm.
          Chemical formulaC₃₁H₅₃N₅O₈S
          Peptide SequenceAc-Pro-Leu-Gly-SCH[CH₂CH(CH₃)₂]-CO-Leu-Gly-OC₂H₅
          Purity≥97% by HPLC
          Solubility50% acetic acid (10 mg/ml) or H₂O (1 mg/ml)
          Storage -20°C
          Protect from light
          Do Not Freeze Ok to freeze
          Special InstructionsUnstable in solution; reconstitute just prior to use.
          Toxicity Standard Handling
          ReferencesWeingarten, H., et al. 1985. Anal. Biochem. 147, 437.
          Weingarten, H., et al. 1985. Biochemistry 24, 6730.

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          Categories

          Life Science Research > Proteins and Enzymes > Substrate & Control Proteins > Protease Substrates