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14-638 | MLCK Protein, active, 10 µg

10 µg  
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      Replacement Information
      Catalogue Number14-638
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionMLCK Protein, active, 10 µg
      OverviewN-terminal 6His-tagged, recombinant, human MLCK, amino acids 1425-1771
      Product Information
      ApplicationActive, N-terminal 6His-tagged, recombinant, human MLCK, amino acids 1425-1771, for use in Kinase Assays.
      Key Applications
      • Kinase Assay
      Biological Information
      Sourceexpressed by baculovirus in Sf21 cells
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Entrez Gene Number
      Entrez Gene SummaryThis gene, a muscle member of the immunoglobulin gene superfamily, encodes myosin light chain kinase which is a calcium/calmodulin dependent enzyme. This kinase phosphorylates myosin regulatory light chains to facilitate myosin interaction with actin filaments to produce contractile activity. This gene encodes both smooth muscle and nonmuscle isoforms. In addition, using a separate promoter in an intron in the 3' region, it encodes telokin, a small protein identical in sequence to the C-terminus of myosin light chain kinase, that is independently expressed in smooth muscle and functions to stabilize unphosphorylated myosin filaments. A pseudogene is located on the p arm of chromosome 3. Four transcript variants that produce four isoforms of the calcium/calmodulin dependent enzyme have been identified as well as two transcripts that produce two isoforms of telokin. Additional variants have been identified but lack full length transcripts.
      Gene Symbol
      • MYLK
      • DKFZp686I10125
      • MLCK108
      • FLJ12216
      • MSTP083
      • KRP
      • MLCK210
      • MLCK
      • Telokin
      Protein TargetMLCK
      Purification MethodNi2+/NTA-agarose
      Target Sub-FamilyCAMK
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q15746 # Calcium/calmodulin-dependent enzyme implicated in smooth muscle contraction via phosphorylation of myosin light chains (MLC). Implicated in the regulation of endothelial as well as vascular permeability. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. Critical participant in signaling sequences that result in fibroblast apoptosis.
      COFACTOR: Magnesium. & Calcium.
      SIZE: 1914 amino acids; 210774 Da
      SUBUNIT: All isoforms including Telokin bind calmodulin.
      TISSUE SPECIFICITY: Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. Telokin has been found in a wide variety of adult and fetal tissues.
      PTM: MLCK is probably down-regulated by phosphorylation.
      SIMILARITY: SwissProt: Q15746 ## Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. & Contains 1 fibronectin type-III domain. & Contains 9 Ig-like C2-type (immunoglobulin-like) domains. & Contains 1 protein kinase domain.
      Molecular Weight43.7kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by phosphorylation of ZIPtide (Catalog # 12-545)
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions6 months at -70°C from date of shipment
      Packaging Information
      Material Size10 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      MLCK, active - 1611522-I 1611522-I
      MLCK, active - 1611522-J 1611522-J
      MLCK, active - 1611522-K 1611522-K


      Reference overviewPub Med ID
      Myosin light chain kinase stimulates smooth muscle myosin ATPase activity by binding to the myosin heads without phosphorylating the myosin light chain.
      Gao, Ying, et al.
      Biochem. Biophys. Res. Commun., 305: 16-21 (2003) 2003

      Show Abstract
      12732190 12732190

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      Product Families


      Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases
      Life Science Research > Proteins and Enzymes > Purified Kinases