A part of MilliporeSigma

RSHMAG-69K | MILLIPLEX MAP Rat Stress Hormone Magnetic Bead Panel - Endocrine Multiplex Assay

Design And Price Your Kit
Click To Print This Page


Replacement Information

Key Specifications Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
ACTH, Corticosterone, Melatonin R Mplex Luminex xMAP
Catalogue NumberRSHMAG-69K
Trade Name
DescriptionMILLIPLEX MAP Rat Stress Hormone Magnetic Bead Panel - Endocrine Multiplex Assay
Background InformationStress is defined as the disruption of homeostasis through physical and/or psychological stimuli (stressors). Physiologically the neuroendocrine system, which includes the hypothalamus, pituitary and adrenal glands, plays a critical role in response to stress. Activated by corticotropin-releasing hormone (CRH) and arginine-vasopressin (AV), the pituitary gland releases ACTH which results in the secretion of cortisol / corticosterone and other glucocorticoids from the adrenal glands. These corticoids involve the whole body in the stress response and are involved in the termination of the stress response by a negative feedback loop to the hypothalamus. Also, corticosterone and melatonin are important in the regulation of the sleep-wake cycle.
Product Information
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Intra-assay: < 10%
  • Inter-assay: < 15%
Panel TypeMAGNETIC Endocrine
ApplicationThis MILLIPLEX MAP Rat Stress Hormone Magnetic Bead Panel is an Endocrine multiplex assay. The analytes available for this multiplex kit are: ACTH, Corticosterone, Melatonin.
Key Applications
  • Multiplexing
Application Notes• This kit may be used for the analysis of Rat ACTH, Corticosterone and/or Melatonin in Rat serum, plasma and tissue/cell supernatant samples.
• Samples size required for duplicate analysis is 15uL (1:4 dilution recommended)
• Overnight incubation is recommended
Biological Information
Species Reactivity
  • Rat
Analytes Available
  • ACTH
  • Corticosterone
  • Melatonin
Cross ReactivityThis assay display insignificant cross-reactivity within the panel
  • ACTH 83%
  • Corticosterone 61%
  • Melatonin 108%
Physicochemical Information
  • ACTH 0.9 pg/mL
  • Melatonin 1.52 pg/mL
  • Corticosterone 2914 pg/mL
Linearity of DilutionContact technical support for this information.
Standard Curve Range
  • ACTH 1.4 - 1,000 pg/mL
  • Melatonin 0.55 to 400 pg/mL
  • Corticosterone 686 - 500,000 pg/mL
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size96-well plate
Transport Information
Supplemental Information



Protocol: Rat Stress Hormone Magnetic Bead Panel



Safety Data Sheet (SDS) 


Reference overviewPub Med ID
Sensitization of restraint-induced corticosterone secretion after chronic restraint in rats: involvement of 5-HT₇ receptors.
García-Iglesias, BB; Mendoza-Garrido, ME; Gutiérrez-Ospina, G; Rangel-Barajas, C; Noyola-Díaz, M; Terrón, JA
Neuropharmacology 71 216-27 2013

Show Abstract
23542440 23542440
Melatonin acts through MT1/MT2 receptors to activate hypothalamic Akt and suppress hepatic gluconeogenesis in rats.
Faria, JA; Kinote, A; Ignacio-Souza, LM; de Araújo, TM; Razolli, DS; Doneda, DL; Paschoal, LB; Lellis-Santos, C; Bertolini, GL; Velloso, LA; Bordin, S; Anhê, GF
American journal of physiology. Endocrinology and metabolism 305 E230-42 2013

Show Abstract
23695212 23695212
mGluR5 ablation in cortical glutamatergic neurons increases novelty-induced locomotion.
Jew, CP; Wu, CS; Sun, H; Zhu, J; Huang, JY; Yu, D; Justice, NJ; Lu, HC
PloS one 8 e70415 2013

Show Abstract
23940572 23940572


Immunoassay and Multiplex Kits: Analyte Quarterly Vol. 2 (EMD)
A closer look at immunoassay platforms. Beyond fit for purpse. Bring your biomarkers to life with EMD Millipore Immunoassay Platform Solutions.
Protein Detection Platform and Services Brochure
The Power of Biomarker Analysis

Technical Info

96 well Template 2 Up
96 well Template Single
Quality Control Ranges: Milliplex® MAP Rat Stress Hormone Kit

Data Sheet

Full Service Custom-built Assays


Multiplex Assays of Fifteen Human Complement Factors