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46-610MAG | MILLIPLEX MAP Phospho p38α/SAPK2 (Thr180/Tyr182) Magnetic Bead MAPmate™ - Cell Signaling Multiplex Assay

46-610MAG
96-well plate  
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      Overview

      Replacement Information

      Key Specifications Table

      Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
      p38α/SAPK2 (Thr180/Tyr182) H, M, R Mplex Luminex xMAP
      Description
      Catalogue Number46-610MAG
      Trade Name
      • MILLIPLEX
      DescriptionMILLIPLEX MAP Phospho p38α/SAPK2 (Thr180/Tyr182) Magnetic Bead MAPmate™ - Cell Signaling Multiplex Assay
      OverviewThe MILLIPLEX® MAP Phospho p38α/SAPK2 (Thr180/Tyr182) Magnetic Bead MAPmate™ pair is used in conjunction with the MILLIPLEX® MAP Cell Signaling Buffer and Detection Kit (Catalog # 48-602MAG) to detect the presence of Phospho p38α/SAPK2 (Thr180/Tyr182) in cell lysates using the Luminex 100™ IS, 200™, HTS, FLEXMAP 3D®, MAGPIX® system. Each MAPmate™ pair is ordered individually and may be combined for simultaneous multiplex analysis of cellular events. The MILLIPLEX® MAP Cell Signaling Buffer and Detection Kit is ordered separately and consists of a common set of reagents needed for performing MAPmate™ assays. The detection assay is a rapid, convenient alternative to Western Blotting and immunoprecipitation procedures. Each kit contains sufficient reagents for 100 individual assays. The MILLIPLEX® MAP HeLa Cell Lysate: Unstimulated included in the MILLIPLEX® MAP Cell Signaling Buffer and Detection Kit may be utilized as an unstimulated control for this target, while the HeLa Cell Lysate: HS/Ars included with this MAPmate™ may be utilized as a stimulated control.

      Important note: For a detailed protocol on Cell Signaling Detection Procedures please see the instruction booklet for the MILLIPLEX® MAP Cell Signaling Buffer and Detection Kit (Catalog # 48-602MAG).
      Materials Required but Not Delivered• MILLIPLEX MAP Cell Signaling Buffer and Detection Kit (Catalog #48-602)
      References
      Product Information
      Detection methodLuminex xMAP
      ConfigurationSingle Plex
      Panel TypeCell Signaling
      Applications
      ApplicationIntracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation and total pathway proteins in tissue and cell lysate samples. Compare Multiplexing results to those of Western blotting.
      Key Applications
      • Multiplexing
      Application Notes• An overnight (4°C) incubation is recommended for best results
      • This assay requires 25 µL diluted cell lysate per well
      • Please consult the buffer compatibility chart or protocol for appropriate buffer choice
      • 1 - 25 μg cell lysate/well (recommended starting concentration is 20 μg)
      Biological Information
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Analytes Available
      • p38α/SAPK2 (Thr180/Tyr182)
      Entrez Gene Number
      UniProt Number
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage for kit components is 2 - 8°C
      Packaging Information
      Material Size96-well plate
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Protocols

      Title
      Phospho CREB (Ser133) Magnetic Bead MAPmate™

      SDS

      Title

      Safety Data Sheet (SDS) 

      Brochure

      Title
      Immunoassay and Multiplex Kits: Analyte Quarterly Vol. 2 (EMD)
      A closer look at immunoassay platforms. Beyond fit for purpse. Bring your biomarkers to life with EMD Millipore Immunoassay Platform Solutions.
      Protein Detection Platform and Services Brochure
      The Power of Biomarker Analysis

      Technical Info

      Title
      96 well Template 2 Up
      96 well Template Single

      Data Sheet

      Title
      Full Service Custom-built Assays

      Posters

      Title
      Multiplex Assays of Fifteen Human Complement Factors

      User Guides

      Title
      Phospho p38α/SAPK2 (Thr180/Tyr182) Magnetic Bead MAPmate™

      Related Products & Applications

      Accessories

      Catalog Number Description  
      48-602MAG MILLIPLEX MAP Cell Signaling Buffer and Detection Kit for Magnetic Beads Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Immunoassays > Multiplexed Immunoassays > Milliplex MAP Assay Products > MAPmates for Cell Signaling
      Life Science Research > Protein Detection and Quantification > Immunoassays > Multiplexed Immunoassays > Milliplex MAP Assay Products > MAPmates for Cell Signaling