A part of MilliporeSigma

MCYP3MAG-74K | MILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Bead Panel III - Immunology Multiplex Assay

Design And Price Your Kit
Click To Print This Page


Replacement Information

Key Specifications Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
TIMP-1, MDC (CCL22), IL-33, IL-27, IL-23, IL-20 M Mplex Luminex xMAP
Catalogue NumberMCYP3MAG-74K
Trade Name
DescriptionMILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Bead Panel III - Immunology Multiplex Assay
OverviewTo identify specific cytokines involved in any inflammatory or immune response, it may be necessary to screen panels of cytokines, often requiring some level of automation and/or high throughput. Magnetic Beads can make the process of automation and high throughput screening easier with features such as walk-away washing. Advantages even outside automation include:
• More flexible plate and plate washer options
• Improved performance with turbid serum/plasma samples
• Assay results comparable to non-magnetic beads
• Automated magnetic washing avoids many problems associated with vacuum filtration washing

Therefore, the MILLIPLEX MAP Mouse Cytokine / Chemokine Magnetic Bead Panel III enables you to focus on the therapeutic potential of cytokines, as well as the modulation of cytokine expression. Coupled with the Luminex® xMAP® platform in a magnetic bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of dozens of analytes simultaneously, dramatically improve productivity.
Background InformationThe immune system is an organized complex network of biological structures and processes that protect against disease. For example, cytokine and chemokines mediate interactions between cells directly, regulating target immune cell responses. These responses result in inflammation where the immune system attempts to eradicate foreign antigens and begin the healing process. Consequently, the inflammatory process plays a key protective role in immunity. In addition cytokine and chemokine research is essential in understanding the immune system and its multi-faceted response to most antigens, as well as disease states such as autoimmune disease, allergic reactions, sepsis and cancer.
Product Information
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Inter-assay (% CV): 10 - 20
  • Intra-assay (% CV): 4 - 9
Panel TypeMAGNETIC Cytokines/Chemokines
ApplicationThe cytokine analytes available for this multiplex kit are: TIMP-1, MDC (CCL22), IL-33, IL-27, IL-23, IL-20.
Key Applications
  • Multiplexing
Application Notes• This is an overnight or two-hour incubation assay; however, for best results an overnight incubation is recommended.
• This assay requires 25 μL of 1:2 dilution of plasma or serum, or 25 μL cell culture supernatant per well.
Biological Information
Species Reactivity
  • Mouse
Analytes Available
  • TIMP-1
  • MDC (CCL22)
  • IL-33
  • IL-27
  • IL-23
  • IL-20
Cross ReactivityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
  • 92% - 118%
Physicochemical Information
  • Refer to kit protocol for sensitivities for individual cytokines.
Standard Curve Range
  • MDC, TIMP-1: 3 – 10,000 pg/mL
  • IL-20: 16 – 50,000 pg/mL
  • IL-23, IL-27, IL-33: 32 – 100,000 pg/mL
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Transport Information
Supplemental Information



Protocol: Mouse Cytokine/Chemokine Magnetic Bead Panel III



Safety Data Sheet (SDS) 


The Power of Biomarker Analysis

Technical Info

96 well Template 2 Up
96 well Template Single
MCYP3MAG-74K xPonent Procotol Template Version 3.1
MCYP3MAG-74K xPonent Procotol Template Version Magpix
MCYP3MAG-74K xPonent Standard Template Version 3.1
Quality Control Ranges: Milliplex Mouse Cytokine Panel III - Catalog # MXMCYP3-74K; Milliplex Mouse Cytokine Magnetic Bead Panel III - Catalog# MCYP3MAG-74K

Data Sheet

Full Service Custom-built Assays