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48-660MAG | MILLIPLEX MAPK/SAPK Signaling 10-Plex Kit - Cell Signaling Multiplex Assay

96-well plate  
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      Replacement Information

      Key Specifications Table

      Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
      ATF2 (Thr71), Erk (Thr185/Tyr187), HSP27 (Ser78), JNK (Thr183/Tyr185), c-Jun (Ser73), MEK1 (Ser222), MSK1 (Ser212), p38 (Thr180/Tyr182), p53 (Ser15), STAT1 (Tyr707) H Mplex Luminex xMAP
      Catalogue Number48-660MAG
      Trade Name
      DescriptionMILLIPLEX MAPK/SAPK Signaling 10-Plex Kit - Cell Signaling Multiplex Assay
      OverviewCells respond to their environment in various ways through intracellular signaling. Signaling through Receptor Tyrosine kinases (RTKs) often promotes increased metabolism and cell growth. Activation of ERK/MAP kinase is one of the key serine/threonine kinases activated via RTK signaling, promoting increased activity of p70 S6 kinase, MSK1, STATs, CREB, and many other signaling intermediates. Other signaling pathways induced via stress (ex. heat shock/arsenite treatment) or death receptors (ex. TNFα) promote p38, JNK, and p53 signaling pathways.
      Product Information
      Detection methodLuminex xMAP
      Precision, %
      • Intra-Assay: 3% - 10%
      • Inter-Assay: 4% - 13%
      Panel TypeCell Signaling
      ApplicationIntracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation or total pathway proteins in tissue and cell lysate samples.
      Key Applications
      • Multiplexing
      Application Notes• An overnight (4°C) incubation is recommended for best results.
      • This assay requires 25 µL diluted cell lysate.
      • This kit must be run using Assay Buffer 2 (provided).
      • 1 - 25 μg cell lysate/well (recommended starting concentration is 20 μg cell lysate/well).
      Biological Information
      SpecificityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
      Species Reactivity
      • Human
      Analytes Available
      • ATF2 (Thr71)
      • Erk (Thr185/Tyr187)
      • HSP27 (Ser78)
      • JNK (Thr183/Tyr185)
      • c-Jun (Ser73)
      • MEK1 (Ser222)
      • MSK1 (Ser212)
      • p38 (Thr180/Tyr182)
      • p53 (Ser15)
      • STAT1 (Tyr707)
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size96-well plate
      Transport Information
      Supplemental Information



      10-Plex MAPK/SAPK Signaling Magnetic Bead Panel



      Safety Data Sheet (SDS) 


      Reference overviewPub Med ID
      Mechanism of Siglec-8-mediated cell death in IL-5-activated eosinophils: role for reactive oxygen species-enhanced MEK/ERK activation.
      Kano, G; Almanan, M; Bochner, BS; Zimmermann, N
      The Journal of allergy and clinical immunology 132 437-45 2013

      Show Abstract
      23684072 23684072


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      Technical Info

      96 well Template 2 Up
      96 well Template Single

      Data Sheet

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