HTMP2MAG-54K | MILLIPLEX MAP Human TIMP Magnetic Bead Panel 2 - Immunology Multiplex Assay

HTMP2MAG-54K
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Overview

Replacement Information

Key Specifications Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
TIMP-1, TIMP-2, TIMP-3, TIMP-4 H Mplex Luminex xMAP
Description
Catalogue NumberHTMP2MAG-54K
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX MAP Human TIMP Magnetic Bead Panel 2 - Immunology Multiplex Assay
OverviewMILLIPLEX® MAP Human TIMP Magnetic Bead Panels enable you to explore the modulation of TIMP expression and the function of the MMP/TIMP in a variety of therapeutic areas. Human TIMP Panel 1 Magnetic Bead kit (TIMP-1, -2) is to be used for serum/plasma samples, while Human TIMP Panel 2 Magnetic Bead kit (TIMP-1, -2, -3, -4) has been designed for tissue/cell culture samples. Both panels are in a convenient “all-in-one” box format to give you the assurance that you will have all the necessary reagents you need to run your assay. Both panels are customizable, allowing you to choose the TIMPs that best fit your needs.
Background InformationTissue Inhibitors of Metalloproteinases (TIMPs) comprise a family of four inhibitors known as TIMP-1, TIMP-2, TIMP-3, and TIMP-4. Each member of the TIMP family has its own distinct profile of metalloproteinase inhibition. The matrix metalloproteinases (MMPs) are a family of zinc proteases involved in the breakdown of extracellular matrix (ECM) in normal physiological processes, such as embryonic development, tissue and bone remodeling, wound healing, tissue morphogenesis, angiogenesis, and tumor metastasis. MMPs are also known to be involved in the cleavage of cell surface receptors, the release of apoptotic ligands (such as the FAS ligand), cell proliferation, differentiation and chemokine in/activation. The normal function of MMPs must be precisely regulated by their endogenous protein inhibitors, TIMPs . TIMPs inhibit MMPs by forming 1:1, non-covalent complexes with MMPs, thereby blocking access of substrates to the MMP catalytic site. Except for inhibition of active MMPs, TIMPs also exhibit several other biochemical and physiological/biological functions such as proMMP activation, cell growth promotion, matrix binding, inhibition of angiogenesis and the induction of apoptosis. Disruption of the MMP/TIMP balance can result in serious diseases such as arthritis, cardiovascular disorders, tumor growth and metastasis.
References
Product Information
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Intra-assay: < 10
  • Inter-assay: < 10
Panel TypeMAGNETIC Cytokines/Chemokines
Applications
ApplicationTIMP Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous analysis of multiple TIMP biomarkers in human cell/tissue culture supernatant samples.
Key Applications
  • Multiplexing
Application Notes• This is a 2-hour or overnight assay requiring 25 μL urine, tissue and cell culture samples. Overnight incubation is recommended.
Biological Information
Species Reactivity
  • Human
Analytes Available
  • TIMP-1
  • TIMP-2
  • TIMP-3
  • TIMP-4
Cross ReactivityNo significant cross-reactivity was observed among analytes in this panel.
Accuracy
  • 77% - 95%
Physicochemical Information
Sensitivity
  • Refer to kit protocol for sensitivities of individual analytes.
Standard Curve Range
  • TIMP-1: 20 - 20,000 pg/mL
  • TIMP-2: 49 - 50,000 pg/mL
  • TIMP-3: 98 - 100,000 pg/mL
  • TIMP-4: 10 - 10,000 pg/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Transport Information
Supplemental Information
Specifications

Documentation

Protocols

Title
Protocol: Human TIMP Magnetic Bead Panel 2

SDS

Title

Safety Data Sheet (SDS) 

References

Reference overviewPub Med ID
Isoproterenol effects evaluated in heart slices of human and rat in comparison to rat heart in vivo.
Herrmann, JE; Heale, J; Bieraugel, M; Ramos, M; Fisher, RL; Vickers, AE
Toxicology and applied pharmacology  274  302-12  2014

Show Abstract
24286936 24286936

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Technical Info

Title
96 well Template 2 Up
96 well Template Single
Quality Control Ranges- MILLIPLEX® MAP Human TIMP Panel 1 Magnetic and Panel 2 Magnetic

Data Sheet

Title
Full Service Custom-built Assays