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HSP1MAG-63K | MILLIPLEX MAP Human Sepsis Magnetic Bead Panel 1 - Immune Response Multiplex Assay

HSP1MAG-63K
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Overview

Replacement Information

Key Specifications Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
MIF, PAI-1 (Total), sFas, sFasL, sICAM-1, sVCAM-1 H Mplex Luminex xMAP
Description
Catalogue NumberHSP1MAG-63K
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX MAP Human Sepsis Magnetic Bead Panel 1 - Immune Response Multiplex Assay
Background InformationSepsis or SIRS (systemic inflammatory response syndrome) results from infection, whether bacterial, viral, fungal or parasitic. According to the CDC, sepsis is the second leading cause of death in non-coronary ICU patients in the U.S. The pathogenesis of sepsis is very complex. Both an innate and acquired immune response to infection interrupts homeostasis with a shift of cytokine expression that increases immunosuppression. In addition migration of the leukocytes in the inflamed tissues secretes a tissue factor leading to the formation of thrombin, also triggering the release of chemokines and adhesion molecules through endothelial cells. Severe sepsis occurs when hypotension or hypoperfusion to one or more organs leads to organ dysfunction, which, in turn, can cause septic shock, multiple organ dysfunction syndrome (MODS) and death.
References
Product Information
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Inter-assay: < 15
  • Intra-assay: < 5
Panel TypeMAGNETIC Immune Response
Applications
ApplicationInflammation/Immunology Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous analysis of multiple sepsis biomarkers in human serum, plasma and cell culture samples.
Key Applications
  • Multiplexing
Application Notes• This assay requires 25 µL of diluted serum or plasma (1:10)
• This assay can also be used with tissue culture samples or homogenates
• Overnight incubation is recommended
Biological Information
Species Reactivity
  • Human
Analytes Available
  • MIF
  • PAI-1 (Total)
  • sFas
  • sFasL
  • sICAM-1
  • sVCAM-1
Cross ReactivityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Accuracy
  • MIF 114%
  • PAI-1 (total) 98%
  • sFas 98%
  • sFasL 106%
  • sICAM-1 104%
  • sVCAM-1 111%
Physicochemical Information
Sensitivity
  • Refer to kit protocol for individual sensitivities.
Standard Curve Range
  • 61–250,000 pg/mL for sVCAM-1 and sICAM-1
  • 12–50,000 pg/mL for sFas, sFasL, MIF and PAI-1
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size96-well plate
Transport Information
Supplemental Information
Specifications

Documentation

Protocols

Title
Protocol: Human Sepsis Magnetic Bead Panel 1

SDS

Title

Safety Data Sheet (SDS) 

Brochure

Title
Immunoassay and Multiplex Kits: Analyte Quarterly Vol. 2 (EMD)
A closer look at immunoassay platforms. Beyond fit for purpse. Bring your biomarkers to life with EMD Millipore Immunoassay Platform Solutions.
Protein Detection Platform and Services Brochure
The Power of Biomarker Analysis

Technical Info

Title
96 well Template 2 Up
96 well Template Single
HSP1MAG-63K xPonent Procotol Template Version Magpix
HSP1MAG-63K xPonent Standard Template Version Magpix
Quality Control Ranges: Milliplex Human Sepsis Magnetic Bead Panel 1 Catalog HSP1MAG-63K

Data Sheet

Title
Full Service Custom-built Assays

Posters

Title
MILLIPLEX® MAP Cell Signaling Wall Chart
Multiplex Assays of Fifteen Human Complement Factors