HNPMAG-35K MILLIPLEX MAP Human Neuropeptide Magnetic Bead Panel - Neuroscience Multiplex Assay

HNPMAG-35K
Design And Price Your Kit

Overview

Replacement Information

Key Specifications Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
α-MSH, β-Endorphin, Neurotensin, Oxytocin, Substance P H Mplex Luminex xMAP
Description
Catalogue NumberHNPMAG-35K
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX MAP Human Neuropeptide Magnetic Bead Panel - Neuroscience Multiplex Assay
OverviewThe MILLIPLEX® MAP Human Neuropeptide Magnetic Bead Panel is to be used for the simultaneous quantification of the following 5 analytes in any combination: α-MSH, β-Endorphin, Neurotensin, Oxytocin, and Substance P. This kit may be used for the analysis of all or any combination of the above analytes in tissue/cell lysate, culture supernatant samples, and cerebrospinal fluid (CSF). Serum or plasma samples may also be used, and will need to be extracted.

An important note about this magnetic conversion panel is that the samples may be extracted using either the acetonitrile precipitation technique or the Waters 96-well HLB extraction plate. Please refer to the protocol for more information on both methods. The necessary components to perform the extractions are not included with the kit.

References:
Am J Med Genet A. 2016 Sep;170(9):2328-33. doi: 10.1002/ajmg.a.37777.
Higher plasma orexin a levels in children with Prader-Willi syndrome compared with healthy unrelated sibling controls.
Manzardo AM, Johnson L, Miller JL, Driscoll DJ, Butler MG.
Background InformationThe central nervous system is a complex environment consisting of billions of neurons and glial support cells. These neurons use chemical signals to communicate information. Neuropeptides are neuronal signaling molecules secreted by the central and peripheral nervous systems, influencing a broad spectrum of biological functions including the regulation of metabolism, reproduction, immunity, and behavior.

Understanding the role of neuropeptides in the pathogenesis of devastating neuronal diseases and behavioral disorders is fundamental. While it is understood that accurate measurement of neuropeptides is important, conventional methods, including RIAs and ELISAs, are not able to simultaneously measure multiple neuropeptides with small sample volume.

EMD Millipore’s MILLIPLEX® MAP Human Neuropeptide Magnetic Bead Panel is a 5-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma, cerebrospinal fluid (CSF), tissue/cell lysate, and culture supernatant samples: α-Melanocyte Stimulating Hormone (α-MSH), β-Endorphin, Neurotensin, Oxytocin, and Substance P.
References
Product Information
Components
  • Human Neuropeptide Standard, 1 vial, lyophilized
  • Human Neuropeptide Quality Control 1, 1 vial, lyophilized
  • Human Neuropeptide Quality Control 2, 1 vial, lyophilized
  • Assay Buffer, 2 bottles, 30 mL ea.
  • Wash Buffer, 10X (0.05% Proclin), 2 bottles, 30 mL ea
  • Human Neuropeptide Primary Antibodies, 1 bottle, 3.5 mL
  • Human Neuropeptide Detection Antibodies, 1 bottle, 5.5 mL
  • Human Neuropeptide Matrix Solution, 1 bottle, 1.5 mL
  • Human Neuropeptide Antibody-Immobilized Magnetic Beads, 20X, 200 uL ea
  • Streptavidin-Phycoerythrin, 1 bottle, 5.5 mL
  • Mixing Bottle, 1 bottle
  • Sample Plate, 96 well format, 1 plate with 2 Foil Plate Sealers
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Inter-assay: < 25
  • Intra-assay: < 15
Panel TypeMAGNETIC Neuroscience
Applications
ApplicationThe analytes available for this multiplex kit are: α-MSH, β-Endorphin, Neurotensin, Oxytocin, Substance P.
Key Applications
  • Multiplexing
Application Notes• This is an overnight assay. Note that this is a competitive assay.
• This assay requires 50 μL extracted serum or plasma sample per well or 50 μL neat CSF or culture supernatant per well.
Biological Information
SpecificityThere was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.
Species Reactivity
  • Human
Analytes Available
  • α-MSH
  • β-Endorphin
  • Neurotensin
  • Oxytocin
  • Substance P
Assay PanelEverything you need in a single kit.
Cross ReactivityThere was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.
Accuracy
  • Spike Recovery: 98% - 118%
Physicochemical Information
Sensitivity
  • 11 - 479 pg/mL (MinDC+2SD).
  • Refer to kit protocol for sensitivities of individual biomarkers.
Standard Curve Range
  • α-MSH: 27 – 20,000 pg/mL
  • β-Endorphine: 69 – 50,000 pg/mL
  • Neurotensin: 41 – 30,000 pg/mL
  • Oxytocin: 55 – 40,000 pg/mL
  • Substance P: 7 – 5,000 pg/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Transport Information
Supplemental Information
Specifications

Documentation

Protocols

Title
Protocol: Human Neuropeptide Magnetic Panel

MILLIPLEX MAP Human Neuropeptide Magnetic Bead Panel - Neuroscience Multiplex Assay SDS

Title

Safety Data Sheet (SDS) 

References

Reference overviewPub Med ID
Immediate effects of spinal manipulation on nitric oxide, substance P and pain perception.
Molina-Ortega, F; Lomas-Vega, R; Hita-Contreras, F; Plaza Manzano, G; Achalandabaso, A; Ramos-Morcillo, AJ; Martínez-Amat, A
Manual therapy  2014

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Brochure

Title
Protein Detection Platform and Services Brochure

Technical Info

Title
96 well Template 2 Up
96 well Template Single
HNPMAG-35K xPonent Protocol Template Version Magpix
Quality Control Ranges- Human Neuropeptide Magnetic Bead Panel

Posters

Title
MILLIPLEX MAP: Exercise, Signaling, and Your Brain (EMD)