HMMP2MAG-55K MILLIPLEX MAP Human MMP Magnetic Bead Panel 2 - Immunology Multiplex Assay

HMMP2MAG-55K
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Overview

Replacement Information

Key Specifications Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
MMP-1, MMP-2, MMP-7, MMP-9, MMP-10 H Mplex Luminex xMAP
Description
Catalogue NumberHMMP2MAG-55K
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX MAP Human MMP Magnetic Bead Panel 2 - Immunology Multiplex Assay
OverviewMMP/TIMP research plays a significant role in achieving a deeper understanding of disease states such as chronic inflammation, cardiovascular disease and cancer. Based on the Luminex xMAP multiplex platform, MILLIPLEX MAP Human MMP Magnetic Bead Panel 2 (MMP-1, -2, -7, -9, -10) will enable you to explore the modulation of and the function of MMP expression in multiple therapeutic areas.
Background InformationThe MMPs (matrix metalloproteinases), a family of zinc proteases responsible for the breakdown of extracellular matrix (ECM), play a key role in normal physiological processes, such as embryonic development and tissue morphogenesis, tissue and bone remodeling, wound healing and angiogenesis. These processes rely on MMPs’ role in the cleavage of cell surface receptors, the release of apoptotic ligands, cell proliferation and differentiation, and chemokine activity modulation. Similar in structure, MMPs are synthesized and secreted as inactive pro-enzymes that require proteolytic cleavage for activation. This process can be mediated by serine proteases or other MMPs. An increase in MMP expression occurs in response to a wide range of stimuli, including adhesion molecules, growth factors, cytokines and hormones. Regulation of MMP activity is controlled primarily by TIMPs (tissue inhibitors of metalloproteinases). Therefore, disruption of the MMP/TIMP balance can result in arthritis, cardiovascular disease and tumor growth and metastasis.
References
Product Information
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Intra-assay:< 10
  • Inter-assay: < 20
Panel TypeMAGNETIC Cytokines/Chemokines
Applications
ApplicationMatrix Metalloproteinase Bead-Based Multiplex Assays using the Luminex technology enable the simultaneous analysis of multiple MMPs biomarkers in human serum, plasma and cell culture samples.
Key Applications
  • Multiplexing
Application Notes• This is a 2-hour-incubation assay requiring 25 μL 1:20 dilution human serum, plasma, tissue/cell lystate and culture supernatant samples.
Biological Information
Species Reactivity
  • Human
Analytes Available
  • MMP-1
  • MMP-2
  • MMP-7
  • MMP-9
  • MMP-10
Cross ReactivityNo significant cross-reactivity was observed among analytes in this panel.
Accuracy
  • 72% - 98%
Physicochemical Information
Sensitivity
  • See protocol for sensitivities of individual analytes.
Standard Curve Range
  • MMP-1: 27 – 20,000 pg/mL
  • MMP-2: 68 -50,000pg/mL
  • MMP-7: 548 – 400,000pg/mL
  • MMP-9: 14 – 10,000pg/mL
  • MMP-10: 27 – 20,000pg/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Transport Information
Supplemental Information
Specifications

Documentation

Protocols

Title
Human MMP Panel 2 Magnetic Bead Kit --96-Well Plate Assay

SDS

Title

Safety Data Sheet (SDS) 

References

Reference overviewPub Med ID
Acquired resistance to zoledronic acid and the parallel acquisition of an aggressive phenotype are mediated by p38-MAP kinase activation in prostate cancer cells.
Milone, MR; Pucci, B; Bruzzese, F; Carbone, C; Piro, G; Costantini, S; Capone, F; Leone, A; Di Gennaro, E; Caraglia, M; Budillon, A
Cell death & disease  4  e641  2013

Show Abstract
23703386 23703386

Brochure

Title
A closer look at immunoassay platforms. Beyond fit for purpse. Bring your biomarkers to life with EMD Millipore Immunoassay Platform Solutions (SigmaMillipore)
Protein Detection Platform and Services Brochure
The Power of Biomarker Analysis

Technical Info

Title
96 well Template 2 Up
96 well Template Single
HMMP2MAG-55K xPonent Standard Template Version Magpix
Quality Control Ranges: MILLIPLEX® MAP Human MMP Panel 2 Magnetic Bead Panel

Data Sheet

Title
Full Service Custom-built Assays

Posters

Title
Biomarkers of the tumor mIcroenvironment
MILLIPLEX MAP: The Immunology of Cancer (EMD)