HGAMMAG-301K MILLIPLEX® MAP Human Isotyping Magnetic Bead Panel - Isotyping Multiplex Assay

HGAMMAG-301K

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Overview

Replacement Information

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This panel is available for custom premix. Talk to your Protein Specialist today!

Key Specifications Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
IgG1, IgG2, IgG3, IgG4, IgA, IgM H Mplex Luminex xMAP
Description
Catalogue NumberHGAMMAG-301K
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX® MAP Human Isotyping Magnetic Bead Panel - Isotyping Multiplex Assay
OverviewEMD Millipore recognizes the need to provide you with the ability to quantitate immunoglobulin classes and subclasses simultaneously. Therefore, our MILLIPLEX MAP Human Isotyping Magnetic Bead Panel, measuring human IgG subclasses (1, 2, 3, 4), IgM and IgA have been designed to enable you to measure both classes and subclasses accurately – all in one well. In addition this panel allows you to choose only those immunoglobulins you need for your experiments.
Background InformationProduced by plasma cells and lymphocytes, immunoglobulins (antibodies) are critically involved in immune response, attaching to antigens and playing a role in their destruction. Immunoglobulins (Ig) can be classified by isotype, classes that differ in function and antigen response due to structure variability. Five major isotypes have been identified in placental mammals: IgM, IgG, IgA, IgE and IgD (B-cell receptor) – all found in normal individuals. Immunoglobulin-deficiency disorders, such as autoimmune disease, some GI conditions and malignancies, are characterized by specific isotype deficiencies or varying concentrations of one or more isotypes. Disease states can range from the absence of one isotype class or subclass to a total deficiency of immunoglobulin classes. In addition, isotyping applications include analyzing hybridomas during antibody generation and vaccine development.
References
Product Information
Components
  • MILLIPLEX® MAP Anti-Human κ and λ Light Chain, PE
  • MILLIPLEX® MAP Human Multi-Immunoglobulin Standard
  • MILLIPLEX® MAP Human Immunoglobulin Positive Control
  • MILLIPLEX® MAP Assay Buffer
  • MILLIPLEX® MAP Wash Buffer, 10X
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Intra-Assay: 6 - 12
  • Inter-Assay: 6 - 10
Panel TypeMAGNETIC Immune Response
Applications
ApplicationIsotyping Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous analysis of multiple immunoglobulins (Ig) in human serum, plasma and cell culture samples.
Key Applications
  • Multiplexing
Application Notes• This kit may be used for the analysis of IgG1, IgG2, IgG3,IgG4, IgA and IgM in human serum, plasma or tissue/cell lysate and culture supernatant samples.
• There are 2 two-hour room-temperature incubations.
• This assay requires 50 µl of 1:16,000 diluted plasma or serum, or 50 μL cell culture supernatant diluted to approximately 1μg/mL Ig per well.
Biological Information
SpecificityCross-reactivity between these antibodies and those against other immunoglobulins is non-detectable or negligible.
Species Reactivity
  • Human
Analytes Available
  • IgG1
  • IgG2
  • IgG3
  • IgG4
  • IgA
  • IgM
Assay PanelEverything you need in a single kit.
Accuracy
  • 88% - 99%
Physicochemical Information
Sensitivity
  • IgG110 ng/mL
  • IgG2 85 ng/mL
  • IgG3 0.09 ng/mL
  • IgG4 0.08 ng/mL
  • IgA 1.5 ng/mL
  • IgM 0.47 ng/mL
Standard Curve Range
  • IgG1 14 – 10,000 ng/mL
  • IgG2 41 – 30,000 ng/mL
  • IgG3 0.2 – 150 ng/mL
  • IgG4 0.4 – 300 ng/mL
  • IgA 2.1 – 1,500 ng/mL
  • IgM 3.4 – 2,500 ng/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Material Package96 Well Plate
Transport Information
Supplemental Information
Specifications

Documentation

Protocols

Title
Protocol: Human Immunoglobulin Isotyping Magnetic Bead Panel

SDS

Title

Safety Data Sheet (SDS) 

References

Reference overviewPub Med ID
Detection of immunoglobulin isotypes from dried blood spots.
Andersen, NJ; Mondal, TK; Preissler, MT; Freed, BM; Stockinger, S; Bell, E; Druschel, C; Louis, GM; Lawrence, DA
Journal of immunological methods  404  24-32  2014

Show Abstract
24333851 24333851

Brochure

Title
A closer look at immunoassay platforms. Beyond fit for purpse. Bring your biomarkers to life with EMD Millipore Immunoassay Platform Solutions (SigmaMillipore)
Protein Detection Platform and Services Brochure
The Power of Biomarker Analysis

Technical Info

Title
96 well Template 2 Up
96 well Template Single
HGAMMAG-301K xPonent Protocol Template Version 3.1
HGAMMAG-301K xPonent Protocol Template Version Magpix
HGAMMAG-301K xPonent Standard Template Version 3.1
HGAMMAG-301K xPonent Standard Template Version Magpix
Quality Control Ranges: Milliplex Human Immunoglobulin Isotyping Kit

Data Sheet

Title
Full Service Custom-built Assays