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HSTCMAG-28SK | MILLIPLEX MAP Human High Sensitivity T Cell Panel - Immunology Multiplex Assay

HSTCMAG-28SK

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Overview

Replacement Information
Replacement InformationMILLIPLEX MAP Human High Sensitivity T Cell Panel, HSTCMAG-28SK replaces MILLIPLEX MAP High Sensitivity Human Cytokine Magnetic Bead Panel, HSCYTMAG-60SK

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This panel is available for custom premix. Talk to your Protein Specialist today!

Key Specifications Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
Fractalkine, GM-CSF, IFNγ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p70), IL-13, IL-17A, IL-21, IL-23, ITAC, MIP-1α, MIP-1β, MIP-3α, TNF-α H Mplex Luminex xMAP
Description
Catalogue NumberHSTCMAG-28SK
ReplacesHSTCMAG-28SK; HSCYTMAG-60SK
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX MAP Human High Sensitivity T Cell Panel - Immunology Multiplex Assay
OverviewReferences:
Brain Behav Immun. 2016 Oct;57:38-46. doi: 10.1016/j.bbi.2016.04.001.
Why sickness hurts: A central mechanism for pain induced by peripheral inflammation.
Karshikoff B, Jensen KB, Kosek E, Kalpouzos G, Soop A, Ingvar M, Olgart Höglund C, Lekander M, Axelsson J.
Background InformationCytokines are immunomodulatory polypeptides that play key roles in both adaptive and innate immune responses. A generic term, “cytokines” includes myokines (produced by muscle cells), lymphokines (produced by activated Th cells), interleukins (acting as mediators between T cells) and chemokines (responsible for T-cell migration). One of the regulatory mechanisms of the immune system, cytokines act at the recognition, activation, or effector phases of an immune response, modulating the development and functional activities of the subtypes of T cells, B cells and myeloid cells. Consequently, research involving cytokines plays a significant role in achieving a deeper understanding of the immune system and its multi-faceted response to most antigens, especially those responses that make up the inflammatory process.

Low levels of inflammation are involved in many clinical and sub-clinical disease states, such as autoimmune disease, cardiovascular disease, diabetes, neurological disorders and cancer. Measuring picogram levels of cytokines, therefore, is critical for understanding the pathogenesis of these diseases.
References
Product Information
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Inter-assay: <15%
  • Intra-assay: <10%
Panel TypeMAGNETIC Cytokines/Chemokines
Applications
ApplicationSimultaneous analyze low levels of cytokine and chemokine biomarker with the High Sensitivity Bead-Based Multiplex Assays using the Luminex technology, in human serum, plasma and cell culture samples.
Key Applications
  • Multiplexing
Application Notes• This is an overnight incubation.
• Because there are unique features concerning sample prep and assay set-up, please read the entire protocol before running the assay. These features are summarized on the next page.
• This kit may be used for the analysis of all or any combination of the Human High Sensitivity T Cell panel analytes in serum, plasma or tissue/cell lysate and culture supernatant.
Biological Information
SpecificityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Species Reactivity
  • Human
Analytes Available
  • Fractalkine
  • GM-CSF
  • IFNγ
  • IL-1β
  • IL-2
  • IL-4
  • IL-5
  • IL-6
  • IL-7
  • IL-8
  • IL-10
  • IL-12 (p70)
  • IL-13
  • IL-17A
  • IL-21
  • IL-23
  • ITAC
  • MIP-1α
  • MIP-1β
  • MIP-3α
  • TNF-α
Cross ReactivityThere is no detectable cross-reactivity within the panel.
Accuracy
  • 98% – 107%
Physicochemical Information
Sensitivity
  • 0.11 - 8.17 pg/mL (Overnight Incubation minDC)
  • Please see kit protocol for individual assay sensitivities.
Linearity of DilutionPlease contact technical service for information
Standard Curve Range
  • Fractalkine: 18.3 – 75,000 pg/mL
  • GM-CSF: 1.22 – 5,000 pg/mL
  • IFNγ: 0.61 – 2,500 pg/mL
  • IL-1β: 0.49 – 2,000 pg/mL
  • IL-2: 0.49 – 2,000 pg/mL
  • IL-4: 1.83 – 7,500 pg/mL
  • IL-5: 0.49 – 2,000 pg/mL
  • IL-6: 0.18 – 750 pg/mL
  • IL-7: 0.37 – 1,500 pg/mL
  • IL-8: 0.31 – 1,250 pg/mL
  • IL-10: 1.46 – 6,000 pg/mL
  • IL-12 (p70): 0.49 – 2,000 pg/mL
  • IL-13: 0.24 – 1,000 pg/mL
  • IL-17A: 0.73 – 3,000 pg/mL
  • IL-21: 0.24 – 1,000 pg/mL
  • IL-23: 7.93 – 32,500 pg/mL
  • ITAC: 1.46 – 6,000 pg/mL
  • MIP-1α: 0.31 – 1,250 pg/mL
  • MIP-1β: 0.92 – 3,750 pg/mL
  • MIP-3α: 0.61 – 2,500 pg/mL
  • TNFα: 0.45 – 1,750 pg/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Transport Information
Supplemental Information
Specifications

Documentation

Protocols

Title
Protocol: Human High Sensitivity T Cell Magnetic Bead Panel

SDS

Title

Safety Data Sheet (SDS) 

References

Reference overviewPub Med ID
Cruciferous vegetable intake is inversely correlated with circulating levels of proinflammatory markers in women.
Jiang, Y; Wu, SH; Shu, XO; Xiang, YB; Ji, BT; Milne, GL; Cai, Q; Zhang, X; Gao, YT; Zheng, W; Yang, G
Journal of the Academy of Nutrition and Dietetics 114 700-8.e2 2014

Show Abstract
24630682 24630682

Brochure

Title
Immunoassay and Multiplex Kits: Analyte Quarterly Vol. 2 (EMD)
A closer look at immunoassay platforms. Beyond fit for purpse. Bring your biomarkers to life with EMD Millipore Immunoassay Platform Solutions.
Protein Detection Platform and Services Brochure
The Power of Biomarker Analysis

Technical Info

Title
96 well Template 2 Up
96 well Template Single
HSTCMAG-28SK xPonent Procotol Template Version 3.1
HSTCMAG-28SK xPonent Procotol Template Version Magpix
HSTCMAG-28SK xPonent Standard Template Version 3.1
HSTCMAG-28SK xPonent Standard Template Version Magpix
Quality Control Ranges: Milliplex Human High Sensitivity T Cell Magnetic Bead Panel
Quantitative multiplex analysis of low-level cytokine expression: MILLIPLEX MAP Human High Sensitivity T Cell Panel

Data Sheet

Title
Full Service Custom-built Assays

Posters

Title
MILLIPLEX MAP: Exercise, Signaling, and Your Brain (EMD)
MILLIPLEX MAP: The Immunology of Cancer (EMD)
Multiplex Assays of Fifteen Human Complement Factors