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HCYTOMAG-60K | MILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Panel - Immunology Multiplex Assay


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Key Specifications Table

Analytes AvailableSpecies ReactivityKey ApplicationsMultiplexing Ordering RequirementsDetection Methods
sCD40L, EGF, FGF-2, Flt-3 ligand, Fractalkine, G-CSF, GM-CSF, GRO, IFN-α2, IFN-γ, IL-1α, IL-1β, IL-1ra, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17A, IP-10, MCP-1, MCP-3, MDC (CCL22), MIP-1α, MIP-1β, PDGF-AB/BB, RANTES, TGF-α, TNF-α, TNF-β, VEGF, Eotaxin/CCL11, PDGF-AA H Mplex Please note: Sample Type must be selected when configuring this kit. RANTES, PDGF-AA and PDGF-AB/AA cannot be plexed with other analytes for serum/plasma. Luminex xMAP
Catalogue NumberHCYTOMAG-60K
Trade Name
DescriptionMILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Panel - Immunology Multiplex Assay
OverviewTo identify specific cytokines involved in any inflammatory or immune response, it might be necessary to screen panels of cytokines, often requiring some level of automation and/or high throughput. Magnetic Beads can make the process of automation and high throughput screening easier with features such as walk-away washing. Advantages even outside automation include:
• More flexible plate and plate washer options
• Improved performance with turbid serum/plasma samples
• Assay results equivalent to non-magnetic beads
• Automated magnetic washing avoids many problems associated with vacuum filtration washing

Therefore, the MILLIPLEX® MAP Human Cytokine / Chemokine panel enables you to focus on the therapeutic potential of cytokines as well as the modulation of cytokine expression. Coupled with the Luminex xMAP® platform in a magnetic bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of dozens of analytes simultaneously, which can dramatically improve productivity.

Mol Psychiatry. 2016 May 24. doi: 10.1038/mp.2016.77.
Autism with intellectual disability is associated with increased levels of maternal cytokines and chemokines during gestation.
Jones KL, Croen LA, Yoshida CK, Heuer L, Hansen R, Zerbo O, DeLorenze GN, Kharrazi M, Yolken R, Ashwood P, Van de Water J.

Aust N Z J Psychiatry. 2016 Jun 16. pii: 0004867416652736.
Inflammatory cytokines in major depressive disorder: A case-control study.
Cassano P, Bui E, Rogers AH, Walton ZE, Ross R, Zeng M, Nadal-Vicens M, Mischoulon D, Baker AW, Keshaviah A, Worthington J, Hoge EA, Alpert J, Fava M, Wong KK, Simon NM.

PLoS One. 2016 Feb 26;11(2):e0149411. doi: 10.1371/journal.pone.0149411. eCollection 2016.
B Cell, Th17, and Neutrophil Related Cerebrospinal Fluid Cytokine/Chemokines Are Elevated in MOG Antibody Associated Demyelination.
Kothur K, Wienholt L, Tantsis EM, Earl J, Bandodkar S, Prelog K, Tea F, Ramanathan S, Brilot F, Dale RC.

Transl Psychiatry. 2016 May 31;6(5):e822. doi: 10.1038/tp.2016.91.
Prenatal immune programming of the sex-dependent risk for major depression.
Gilman SE, Cherkerzian S, Buka SL, Hahn J, Hornig M, Goldstein JM.
Background Information“Cytokine” is a general term used for a diverse group of soluble proteins and peptides which act as regulators under both normal and pathological conditions to modulate the functional activities of individual cells and tissues. These proteins also mediate direct interactions between cells and regulate processes taking place in the extracellular environment. Cytokines differ from hormones in that they act on a wider spectrum of target cells. Also, unlike hormones, they are not produced by specialized cells which are organized in specialized glands. The cytokine group of proteins includes lymphokines, interferons, colony stimulating factors and chemokines. Cytokine and chemokine research plays a significant role in achieving a deeper understanding of the immune system and its multi-faceted response to most antigens, as well as disease states such as inflammatory disease, allergic reactions, irritable bowel disease (IBD), sepsis, and cancer.
Product Information
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Intra-assay: 2 - 13%
  • Inter-assay: 5 - 19%
Multiplex Ordering RequirementsPlease note: Sample Type must be selected when configuring this kit. RANTES, PDGF-AA and PDGF-AB/AA cannot be plexed with other analytes for serum/plasma.
Panel TypeMAGNETIC Cytokines/Chemokines
ApplicationSimultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in mouse serum, plasma and cell culture samples.
Key Applications
  • Multiplexing
Application Notes• This is an overnight or two-hour incubation assay; however, for best results an overnight incubation is recommended.

• This assay requires 25 µl of neat plasma or serum, or 25 μL cell culture supernatant per well.

• This kit may be used for the analysis of all or any combination of the cytokines and chemokines in serum, plasma or tissue/cell lysate and culture supernatant samples with the exception of RANTES, PDGF-AA and PDGF-AB/BB.

• RANTES, PDGF-AA and PDGF-AB/BB cannot be plexed with other cytokines in this panel due to a required 1:100 dilution of plasma/serum samples.
Biological Information
Species Reactivity
  • Human
Analytes Available
  • sCD40L
  • EGF
  • FGF-2
  • Flt-3 ligand
  • Fractalkine
  • G-CSF
  • GM-CSF
  • GRO
  • IFN-α2
  • IFN-γ
  • IL-1α
  • IL-1β
  • IL-1ra
  • IL-2
  • IL-3
  • IL-4
  • IL-5
  • IL-6
  • IL-7
  • IL-8
  • IL-9
  • IL-10
  • IL-12 (p40)
  • IL-12 (p70)
  • IL-13
  • IL-15
  • IL-17A
  • IP-10
  • MCP-1
  • MCP-3
  • MDC (CCL22)
  • MIP-1α
  • MIP-1β
  • TGF-α
  • TNF-α
  • TNF-β
  • VEGF
  • Eotaxin/CCL11
Cross ReactivityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
  • 87% - 107%
Physicochemical Information
  • Refer to kit protocol for sensitivities for individual cytokines/chemokines.
Standard Curve Range
  • 3.2–10,000 pg/mL
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Material Package96 well plate
Transport Information
Supplemental Information



Protocol: Human Cytokine / Chemokine Magnetic Bead Panel



Safety Data Sheet (SDS) 

References | 19 Available | See All References

Reference overviewPub Med ID
Marked and persistent eosinophilia in the absence of clinical manifestations.
Chen, YY; Khoury, P; Ware, JM; Holland-Thomas, NC; Stoddard, JL; Gurprasad, S; Waldner, AJ; Klion, AD
The Journal of allergy and clinical immunology 133 1195-202 2014

Show Abstract
23987798 23987798
Isoproterenol effects evaluated in heart slices of human and rat in comparison to rat heart in vivo.
Herrmann, JE; Heale, J; Bieraugel, M; Ramos, M; Fisher, RL; Vickers, AE
Toxicology and applied pharmacology 274 302-12 2014

Show Abstract
24286936 24286936
Dry eye exacerbation in patients exposed to desiccating stress under controlled environmental conditions.
López-Miguel, A; Tesón, M; Martín-Montañez, V; Enríquez-de-Salamanca, A; Stern, ME; Calonge, M; González-García, MJ
American journal of ophthalmology 157 788-798.e2 2014

Show Abstract
24412126 24412126
Intra- and inter-day variation of cytokines and chemokines in tears of healthy subjects.
Benito, MJ; González-García, MJ; Tesón, M; García, N; Fernández, I; Calonge, M; Enríquez-de-Salamanca, A
Experimental eye research 120 43-9 2014

Show Abstract
24412438 24412438
Maternal obesity is associated with a lipotoxic placental environment.
Saben, J; Lindsey, F; Zhong, Y; Thakali, K; Badger, TM; Andres, A; Gomez-Acevedo, H; Shankar, K
Placenta 35 171-7 2014

Show Abstract
24484739 24484739
rs2243268 and rs2243274 of Interleukin-4 (IL-4) gene are associated with reduced risk for extrapulmonary and severe tuberculosis in Chinese Han children.
Qi, H; Sun, L; Jin, YQ; Shen, C; Chu, P; Wang, SF; Yin, QQ; Qi, Z; Xu, F; Jiao, WW; Wu, XR; Tian, JL; Xiao, J; Shen, AD
Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases 23 121-8 2014

Show Abstract
24518693 24518693
Cytokines and hs-CRP levels in individuals treated with low-dose aspirin for cardiovascular prevention: a population-based study (CoLaus Study).
Vaucher, J; Marques-Vidal, P; Waeber, G; Vollenweider, P
Cytokine 66 95-100 2014

Show Abstract
24594292 24594292
Effects of rhinovirus species on viral replication and cytokine production.
Nakagome, K; Bochkov, YA; Ashraf, S; Brockman-Schneider, RA; Evans, MD; Pasic, TR; Gern, JE
The Journal of allergy and clinical immunology 134 332-341.e10 2014

Show Abstract
24636084 24636084
Pessimistic orientation in relation to telomere length in older men: the VA normative aging study.
Ikeda, A; Schwartz, J; Peters, JL; Baccarelli, AA; Hoxha, M; Dioni, L; Spiro, A; Sparrow, D; Vokonas, P; Kubzansky, LD
Psychoneuroendocrinology 42 68-76 2014

Show Abstract
24636503 24636503
Effect of progestins on immunity: medroxyprogesterone but not norethisterone or levonorgestrel suppresses the function of T cells and pDCs.
Huijbregts, RP; Michel, KG; Hel, Z
Contraception 90 123-9 2014

Show Abstract
24674041 24674041


Immunoassay and Multiplex Kits: Analyte Quarterly Vol. 2 (EMD)
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Technical Info

96 well Template 2 Up
96 well Template Single
Good Correlation Between Measured Cytokine Levels Using Multiplexed Bead-based Detection and ELISA Platforms
HCYTOMAG-60K xPonent Procotol Template Version 3.1
HCYTOMAG-60K xPonent Procotol Template Version Magpix
HCYTOMAG-60K xPonent Standard Template Version 3.1
HCYTOMAG-60K xPonent Standard Template Version Magpix
Quality Control Ranges: Milliplex Human Cytokine / Chemokine Immunoassay

Data Sheet

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