HADK1MAG-61K MILLIPLEX MAP Human Adipokine Magnetic Bead Panel 1 - Endocrine Multiplex Assay

HADK1MAG-61K
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Overview

Replacement Information

Key Specifications Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
Adiponectin, Adipsin, Lipocalin-2/NGAL, PAI-1 (Total), Resistin H Mplex Luminex xMAP
Description
Catalogue NumberHADK1MAG-61K
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX MAP Human Adipokine Magnetic Bead Panel 1 - Endocrine Multiplex Assay
OverviewThe MILLIPLEX® MAP Human Adipokine Magnetic Bead Panel 1 is a 5-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum or plasma samples: Adiponectin, Adipsin, Lipocalin-2/NGAL, Total PAI-1 and Resistin. This kit uses a 96-well format, contains a lyophilized standard cocktail, 2 quality controls and can measure up to 38 serum or plasma samples in duplicate.

References:
Cephalalgia. 2016 May 18. pii: 0333102416650705.
Evidence for potential involvement of pro-inflammatory adipokines in the pathogenesis of idiopathic intracranial hypertension.
Samancı B, Samancı Y, Tüzün E, Altıokka-Uzun G, Ekizoğlu E, İçöz S, Şahin E, Küçükali Cİ, Baykan B.
Background InformationThe role of white adipose tissue was once thought to be limited to energy storage and internal organ protection. The discovery of Leptin secretion from Adipocytes in 1994 led to the recognition that white adipose tissue is involved in a variety of metabolic and physiological processes. Adipocytes secrete a number of hormones called Adipokines with functions that include appetite and energy balance, insulin sensitivity and lipid metabolism. The Adipokine Adiponectin is involved in the regulation of lipid and glucose metabolism, influencing the body’s response to insulin. Adiponectin's anti-inflammatory effects on the cellular lining of blood vessel walls may help explain the association of high Adiponectin levels with the reduced risk of heart attack.
References
Product Information
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Adiponectin: Inter-assay <10% CV Intra-assay <15% CV
  • Adipsin: Inter-assay <10% CV Intra-assay <10% CV
  • Lipocalin-2/NGAL: Inter-assay <10% CV Intra-assay <15% CV
  • PAI-1 (total): Inter-assay <10% CV Intra-assay <20% CV
  • Resistin: Inter-assay <10% CV Intra-assay <20% CV
Panel TypeMAGNETIC Metabolism
Applications
ApplicationThe Human Adipokine Panel 1, using the Luminex xMAP technology, enables the simultaneous analysis of five biomarkers in human serum or plasma samples.
Key Applications
  • Multiplexing
Application NotesFor optimal sensitivity, use an overnight (16-18 hour) incubation. Serum and plasma samples require (recommend) a 1:400 dilution with Assay Buffer. Each well requires 25 μL of sample, standard or control.
Biological Information
SpecificityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Species Reactivity
  • Human
Analytes Available
  • Adiponectin
  • Adipsin
  • Lipocalin-2/NGAL
  • PAI-1 (Total)
  • Resistin
Assay PanelEverything you need in a single kit.
Cross ReactivityEach antibody pair in a panel is highly specific and displays insignificant cross-reactivity to other analytes in the panel.
Accuracy
  • Adiponectin 89%
  • Adipsin 98%
  • Lipocalcin 2/NGAL 91%
  • PAI-1 75%
  • Resistin 87%
Physicochemical Information
Sensitivity
  • MinDC+2SD
  • Adiponectin: 21 pg/mL
  • Adipsin: 10 pg/mL
  • Lipocalin-2/NGAL: 3.5 pg/mL
  • PAI-1: 5.8 pg/mL
  • Resistin: 4.4 pg/mL
Linearity of DilutionPlease contact technical support for this information
Standard Curve Range
  • Adiponectin: 26-400,000 pg/mL
  • Adipsin: 13-200,000 pg/mL
  • Lipocalin-2/NGAL: 3.2-50,000 pg/mL
  • PAI-1 (total): 9.6-150,000 pg/mL
  • Resistin: 6.4-100,000 pg/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Material Package96-well plate
Transport Information
Supplemental Information
Specifications

Documentation

Protocols

Title
Protocol: Human Adipokine Magnetic Bead Panel 1 Cat. # HADK1MAG-61K

SDS

Title

Safety Data Sheet (SDS) 

References

Reference overviewPub Med ID
Growth differentiation factor 15 and cardiovascular events in patients with stable ischemic heart disease (The Heart and Soul Study).
Schopfer, DW; Ku, IA; Regan, M; Whooley, MA
American heart journal  167  186-192.e1  2014

Show Abstract
24439979 24439979
A pilot study of active vitamin D administration and insulin resistance in African American patients undergoing chronic hemodialysis.
Hung, AM; Sundell, MB; Plotnikova, NE; Bian, A; Shintani, A; Ellis, CD; Siew, ED; Ikizler, TA
Journal of renal nutrition : the official journal of the Council on Renal Nutrition of the National Kidney Foundation  23  185-93  2013

Show Abstract
22959781 22959781

Brochure

Title
A closer look at immunoassay platforms. Beyond fit for purpse. Bring your biomarkers to life with EMD Millipore Immunoassay Platform Solutions (SigmaMillipore)
Protein Detection Platform and Services Brochure
The Power of Biomarker Analysis

Technical Info

Title
96 well Template 2 Up
96 well Template Single
HADK1MAG-61K xPonent Protocol Template Version 3.1
HADK1MAG-61K xPonent Protocol Template Version Magpix
HADK1MAG-61K xPonent Standard Template Version 3.1
HADK1MAG-61K xPonent Standard Template Version Magpix
Quality Control Ranges: Milliplex® Human Adipokine Magnetic Bead Panel 1

Data Sheet

Title
Full Service Custom-built Assays