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13-110 | MBP, Dephosphorylated

5 mg  
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      Replacement Information
      Catalogue Number13-110
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionMBP, Dephosphorylated
      OverviewMBP, Dephosphorylated is made from MBP (catalog #13-104) which is basally phosphorylated and treated with lambda protein phosphatase (catalog #14-405) for complete dephosphorylation.
      Product Information
      Presentation10mM MOPS, pH 7.0, 128mM MnCl2, 641mM EDTA, 1.134μg inactive lambda phosphatase and 0.05% sodium azide
      Key Applications
      • Enzyme Assay
      Application NotesMyelin basic protein purified from bovine brain and de-phosphorylated using Lambda protein phosphatase. No detectable endogenous phosphorylation as determined by immunoblotting 1 microgram of MBP with 1 microgram/ml anti phospho MBP.
      Biological Information
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called "Golli-MBP") that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes.
      Gene Symbol
      • MBP
      • MGC99675
      • Phosphorylation
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P02686 # The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non- classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T- cells and neural cells. Differential splicing events combined to optional post-translational modifications give a wide spectrum of isomers, each of them having maybe a specialized function. Induces T-cell proliferation.
      SIZE: 304 amino acids; 33117 Da
      SUBUNIT: Homodimer; isoform 3 exists as a homodimer.
      SUBCELLULAR LOCATION: Myelin membrane; Peripheral membrane protein; Cytoplasmic side. Note=Cytoplasmic side of myelin.
      TISSUE SPECIFICITY: MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.DEVELOPMENTAL STAGE: Expression turns on abruptly in fetus of 14 to 16 weeks. Even smaller isoforms seem to be produced during embryogenesis, some of these persisting in the adult. Expression of isoform MBP2 is more evident at 16 weeks and its relative proportion declined thereafter.
      PTM: Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases in aging, making the protein more cationic. & The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6). & Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated.
      DISEASE: SwissProt: P02686 # The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease).
      SIMILARITY: SwissProt: P02686 ## Belongs to the myelin basic protein family.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated as a substrate in a kinase assay using MAP Kinase 2/Erk2 (Catalog # 14-173)
      Sales RestrictionsThis product is derived from bovine source. Export of this product to certain countries may be restricted. Please contact Customer Service or your local distributor to inquire about product availability and export options.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size5 mg
      Transport Information
      Supplemental Information


      Certificates of Analysis

      TitleLot Number
      MBP, Dephosphorylated 2459576
      MBP, Dephosphorylated (produced from bovine brain) - 2118885 2118885
      MBP, Dephosphorylated (produced from bovine brain) - 2272621 2272621
      MBP, Dephosphorylated (produced from bovine brain) - 2433355 2433355
      MBP, Dephosphorylated (produced from bovine brain) - 2446764 2446764
      MBP, Dephosphorylated (produced from bovine brain) 2477880
      MBP, Dephosphorylated (produced from bovine brain) - 2103523 2103523
      MBP, Dephosphorylated (produced from bovine brain) - 2105943 2105943
      MBP, Dephosphorylated (produced from bovine brain) - 2204412 2204412
      MBP, Dephosphorylated (produced from bovine brain) - 2210393 2210393
      MBP, Dephosphorylated (produced from bovine brain) - DAM1475041 DAM1475041
      MBP, Dephosphorylated (produced from bovine brain) - DAM1832540 DAM1832540
      MBP, Dephosphorylated (produced from bovine brain) - JBC1936137 JBC1936137
      MBP, Dephosphorylated - 18260 18260
      MBP, Dephosphorylated - 19576 19576
      MBP, Dephosphorylated - 19979 19979
      MBP, Dephosphorylated - 2045577 2045577
      MBP, Dephosphorylated - 2067832 2067832
      MBP, Dephosphorylated - 20717 20717
      MBP, Dephosphorylated - 20843 20843
      MBP, Dephosphorylated - 23575 23575
      MBP, Dephosphorylated - 24952 24952
      MBP, Dephosphorylated - 2557647 2557647
      MBP, Dephosphorylated - 27568 27568
      MBP, Dephosphorylated - 27845 27845
      MBP, Dephosphorylated - 28053A 28053A
      MBP, Dephosphorylated - DAM1594137 DAM1594137
      MBP, Dephosphorylated - DAM1698737 DAM1698737
      MBP, Dephosphorylated - DAM1764486 DAM1764486
      MBP, Dephosphorylated - DAM1791369 DAM1791369
      MBP, Dephosphorylated - JBC1886235 JBC1886235
      MBP, Dephosphorylated -2605386 2605386
      MBP, Dephosphorylated -2716197 2716197


      Reference overviewApplicationPub Med ID
      Discovery of potent small molecule inhibitors of DYRK1A by structure-based virtual screening and bioassay.
      Di Wang,Fei Wang,Yexiong Tan,Liwei Dong,Lei Chen,Weiliang Zhu,Hongyang Wang
      Bioorganic & medicinal chemistry letters 22 2012

      Show Abstract
      22154664 22154664
      Human Mob proteins regulate the NDR1 and NDR2 serine-threonine kinases
      Devroe, E., et al
      J Biol Chem, 279:24444-51 (2004) 2004

      Kinase Assay15067004 15067004
      Cloning of MASK, a novel member of the mammalian germinal center kinase III subfamily, with apoptosis-inducing properties
      Dan, I., et al
      J Biol Chem, 277:5929-39 (2002) 2002

      Kinase Assay11741893 11741893
      Thrombospondin stimulates focal adhesion disassembly through Gi- and phosphoinositide 3-kinase-dependent ERK activation
      Orr, A. W., et al
      J Biol Chem, 277:20453-60 (2002) 2002

      Kinase Assay11923291 11923291
      Identification and characterization of a novel sucrose-non-fermenting protein kinase/AMP-activated protein kinase-related protein kinase, SNARK
      Lefebvre, D. L., et al
      Biochem J, 355:297-305 (2001) 2001

      Kinase Assay11284715 11284715
      Structural and functional characterization of recombinant human cellular retinaldehyde-binding protein.
      J W Crabb,A Carlson,Y Chen,S Goldflam,R Intres,K A West,J D Hulmes,J T Kapron,L A Luck,J Horwitz,D Bok
      Protein science : a publication of the Protein Society 7 1998

      Show Abstract Full Text Article
      9541407 9541407
      Activation of multiple protein kinases during the burst in protein phosphorylation that precedes the first meiotic cell division in Xenopus oocytes.
      Cicirelli, M F, et al.
      J. Biol. Chem., 263: 2009-19 (1988) 1988

      2448302 2448302
      Cyclic AMP decreases the phosphorylation state of myelin basic proteins in rat brain cell cultures.
      Ulmer, J B, et al.
      J. Biol. Chem., 262: 1748-55 (1987) 1987

      Show Abstract
      2433287 2433287
      Identification of multiple in vivo phosphorylation sites in rabbit myelin basic protein.
      Martenson, R E, et al.
      J. Biol. Chem., 258: 930-7 (1983) 1983

      Show Abstract
      6185481 6185481

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