A part of MilliporeSigma

14-439 | MAP Kinase 1/Erk1 Protein, active, 10 µg

10 µg  Also available in 250μg size as catalog # 14-439M - Call for pricing and availability.
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
Request Pricing
Limited AvailabilityLimited Availability
Limited Quantities Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service


      Contact Customer Service

      Click To Print This Page


      Replacement Information
      Catalogue Number14-439
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionMAP Kinase 1/Erk1 Protein, active, 10 µg
      OverviewRecombinant, full-length, human MAP Kinase 1/Erk1 containing an N-terminal GST tag
      Product Information
      ApplicationActive, recombinant, full-length, human MAP Kinase 1/Erk1 containing an N-terminal GST tag, for use in Kinase Assays.
      Key Applications
      • Kinase Assay
      Biological Information
      SourceExpressed in E. coli
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene.
      Gene Symbol
      • MAPK1
      • p40
      • PRKM2
      • MAPK2
      • PRKM1
      • p42-MAPK
      • p38
      • p41
      • ERK
      • ERK-2
      • ERT1
      • P42MAPK
      • p41mapk
      • ERK2
      Protein TargetMAPK1
      Purification MethodPurified by glutathione-agarose. Activated with MEK1 and repurified on Ni2+/NTA agarose
      Target Sub-FamilyCMGC
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P28482 # Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
      COFACTOR: Magnesium (By similarity).
      SIZE: 360 amino acids; 41390 Da
      SUBUNIT: Interacts with MORG1 (By similarity). Binds to HIV-1 Nef through its SH3 domain. This interaction inhibits its tyrosine- kinase activity. Interacts with HSF4.
      DOMAIN: SwissProt: P28482 The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
      PTM: Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme.
      SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. & Contains 1 protein kinase domain.
      Molecular Weight69.9kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by phosphorylation of MBP
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions1 year at -20°C
      Packaging Information
      Material Size10 µg
      Material PackageAlso available in 250μg size as catalog # 14-439M - Call for pricing and availability.
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      MAP kinase 1, active - 1783740-I 1783740-I
      MAP kinase 1, active - 1783740-J 1783740-J
      MAP kinase 1, active - 2052233-A 2052233-A
      MAP kinase 1, active - 2052233-B 2052233-B


      Reference overviewApplicationPub Med ID
      Vav1 transduces T cell receptor signals to the activation of the Ras/ERK pathway via LAT, Sos, and RasGRP1.
      Reynolds, Lucinda F, et al.
      J. Biol. Chem., 279: 18239-46 (2004) 2004

      Show Abstract
      Immunoblotting (Western)14764585 14764585
      Signal transduction pathways involved in phosphorylation and activation of p70S6K following exposure to UVA irradiation
      Zhang, Y., et al
      J Biol Chem, 276:20913-23 (2001) 2001

      Kinase Assay11279232 11279232

      Related Products & Applications

      Alternative Packsize

      Catalog Number Description  
      14-439M MAP Kinase 1/Erk1 Protein, active, 250 µg Show Pricing & Availability


      Catalog Number Description  
      20-101 Protein Dilution Buffer A Show Pricing & Availability

      Product Families


      Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases
      Life Science Research > Proteins and Enzymes > Purified Kinases