71230 Lysonase™ Bioprocessing Reagent

71230
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      Overview

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      71230-3
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          Glass bottle 0.2 ml
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          71230-4
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              Plastic ampoule 1 ml
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              71230-5
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                  Plastic ampoule 5 ml
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                  Description
                  Overview

                  Lysonase™ Bioprocessing Reagent is an optimized, ready-to-use blend of rLysozyme™ Solution and Benzonase Nuclease. rLysozyme Solution contains a highly purified and stabilized recombinant lysozyme with specific activity 250 times greater than that of chicken egg white lysozyme. Benzonase Nuclease is a genetically engineered nonspecific endonuclease that degrades all forms of DNA and RNA (single stranded, double stranded, circular, linear), reducing extract viscosity and increasing protein yield. The combined activities of rLysozyme and Benzonase Nuclease significantly increase protein extraction efficiency and facilitate downstream processing of protein extracts. For efficient protein extraction with BugBuster® Protein Extraction Reagent, use 10 µl Lysonase per 1 g cell paste. For efficient protein extraction with PopCulture® Reagent, add 2 µl Lysonase per 1 ml culture. Store at –20°C.

                  Catalogue Number71230
                  Brand Family Novagen®
                  References
                  Product Information
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                  Biological Information
                  Physicochemical Information
                  Dimensions
                  Materials Information
                  Toxicological Information
                  Safety Information according to GHS
                  Safety Information
                  Product Usage Statements
                  Storage and Shipping Information
                  Ship Code Blue Ice Only
                  Toxicity Standard Handling
                  Storage -20°C
                  Do not freeze Ok to freeze
                  Packaging Information
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                  Supplemental Information
                  Specifications

                  Documentation

                  Lysonase™ Bioprocessing Reagent SDS

                  Title

                  Safety Data Sheet (SDS) 

                  Lysonase™ Bioprocessing Reagent Certificates of Analysis

                  TitleLot Number
                  71230

                  Brochure

                  Title
                  Protein Purification and Detection Tools
                  Protein Purification and Preparation

                  Citations

                  Title
                • Johannes Mullegger, et al. (2005) Engineering of a thioglycoligase: randomized mutagenesis of the acid-base residue leads to the identification of improved catalysts. Protein Engineering Design and Selection 18, 33-40.
                • Dirk Hoffmeister and Jon S. Thorson. (2004) Mechanistic implications of Escherichia coli galactokinase structure-based engineering. ChemBioChem 5, 989-992.
                • Dirk Hoffmeister, et al. (2003) Creation of the first anomeric D/L-sugar kinase by means of directed evolution. Procedings of the National Academy of Science 100, 13184-13189.
                • User Protocols

                  Title
                  TB361 Lysonase™ Bioprocessing Reagent